A5020689710- Advanced NMR Techniques and Applications
- Crystallization and Solubility Studies
- Solid-state spectroscopy and crystallography
- NMR spectroscopy and applications
- Microbial Natural Products and Biosynthesis
- Advanced MRI Techniques and Applications
- X-ray Diffraction in Crystallography
- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- Protein purification and stability
- Microbial Metabolism and Applications
- Fungal Biology and Applications
- Glycosylation and Glycoproteins Research
- Synthesis and Characterization of Heterocyclic Compounds
- Nuclear Physics and Applications
- Cancer Genomics and Diagnostics
- Protein Structure and Dynamics
- Bioactive Compounds and Antitumor Agents
- Lipid Membrane Structure and Behavior
- Nanopore and Nanochannel Transport Studies
- Melanoma and MAPK Pathways
- Marine Sponges and Natural Products
- Bone health and osteoporosis research
- Radiopharmaceutical Chemistry and Applications
Technical University of Munich
2013-2024
Fischer (Germany)
2022
Goethe University Frankfurt
2021
Center for Integrated Protein Science Munich
2018
University of Würzburg
1993-1995
ETH Zurich
1989
University of Tübingen
1988
Recently, proton-detected magic-angle spinning (MAS) solid-state nuclear magnetic resonance (NMR) spectroscopy has become an attractive tool to study the structure and dynamics of insoluble proteins at atomic resolution. The sensitivity employed multidimensional experiments can be systematically improved when both transversal components magnetization are transferred simultaneously after evolution period. method preservation equivalent pathways been explored in solution-state NMR; however, it...
Fullerenes and diamondoids are at the core of nanoscience. Comparable monodisperse silicon analogues scarce. Herein, we report synthesis parent siladodecahedrane, which represents largest Platonic solid. It shares its pattern pentagonal faces with smallest fullerene, C20, saturated, H-terminated skeleton diamondoids. Similar to endofullerenes, cage encapsulates a chloride ion ([Cl@Si20H20]-); similar diamondoids, Si-H termini offer wealth opportunities for further functionalization. Mere...
We have recently introduced optimal-control derived pulse sequences for sensitivity-enhanced heteronuclear correlation NMR experiments of solid proteins. Preservation equivalent coherence transfer pathways using transverse-mixing pulses (TROP) in multidimensional schemes allows to increase the sensitivity by more than a factor 2 per each indirect dimension. In this article, we present homonuclear CA-CO elements (homoTROP) that are based on dipolar interactions and achieve similar gains as...
Highly efficient optimal control experiments for routine studies of solid proteins by NMR using various MAS frequencies.
The condensation of <sc>l</sc>-cysteine and aryl nitriles to (<i>R</i>)-2-aryl-4,5-dihydrothiazole-4-carboxylic acids in buffered media was reinvestigated. Pure products (yields 58–95%; up 99% ee) were obtained a NaHCO<sub>3</sub>/NaOH-buffered aqueous alcoholic medium.
The Esmeraldines A and B, Green Pigments from Streptomyces antibioticus , Strain Tü 2706 From a strain of Sterptomyces 2706, two dark green substances, the esmeraldines 4 3 respectively, were isolated. structural elucidation was carried out by spectroscopic comparison with some simpler phenazines obtained same organism, saphenamycin ( 1 ) related compounds, series chemical transformations. Esmeraldin B is 6‐methylsalicylic acid ester, esmeraldin mixture higher saturated unsaturated fatty...
Protein-based drugs are a mainstay of modern medicine. In contrast to antibodies, most these need highly individualized production processes which often limits their development. Here, we develop an immunoglobulin domain tag (i-Tag), can be fused any protein interest. This is made linear arrangement antibody light chain constant domains. It enhances expression as well secretion the fusion partner and allows for simple purification several structurally functionally distinct proteins....
Despite their importance for antibody architecture and design, the principles governing domain stability are still not understood in sufficient detail. Here, to address this question, we chose a from invariant part of IgG, CH2 domain. We found that compared with other Ig domains, isolated is surprisingly unstable monomer, exhibiting melting temperature ∼44 °C. further show presence an additional C-terminal lysine variant substantially increases by ∼14 °C relative WT. To explore molecular...
Abstract In cancer research, tumor spheroids are a well established system to study metabolism resembling the situation in vivo more closely cell monolayers. Spherical aggregates of malignant melanoma cells (MV3) and their invasion into rat brain have been investigated by quantitative NMR microscopy. Relaxation times ( T 1 , 2 ) diffusion parameter images were acquired with an in‐plane resolution 14 × μm . The authors able demonstrate that morphology can be visualized on these maps. contrast...
Abstract The title condensation in buffered aqueous alcoholic medium is reinvestigated and it can be shown that no undesired hydrolysis or racemization observed.
Abstract In contrast to saphenamycin (III) esmeraldin A (isolated as a mixture of higher saturated and unsaturated fatty acid esters (Ia)‐(Id) esmeraldic acid) B (II) do not reveal antibacterial activity.