A5042021205- Protein Structure and Dynamics
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Fermentation and Sensory Analysis
- Nanopore and Nanochannel Transport Studies
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
Fischer (Germany)
2022-2024
Technical University of Munich
2018-2024
Glycosylation mediated by UDP-dependent glycosyltransferase (UGT) is one of the most common reactions for biosynthesis small molecule glycosides. As glycosides have various biological roles, we characterized UGT genes from grapevine (Vitis vinifera). In silico analysis VvUGT that were highly expressed in leaves identified UGT92G6 which showed sequence similarity to both monosaccharide and disaccharide glucoside-forming transferases. The recombinant glucosylated phenolics, among them caffeic...
High-resolution structural NMR analyses of membrane proteins are challenging due to their large size, resulting in broad resonances and strong signal overlap. Among the isotope labeling methods that can remedy this situation, segmental is a suitable strategy simplify spectra retain high-resolution information. However, protein ligation within integral complicated since hydrophobic fragments insoluble, removal side-products elaborate. Here, we show stabilized split-intein system be used for...