A5013459995- Sleep and Wakefulness Research
- Circadian rhythm and melatonin
- Sleep and related disorders
- EEG and Brain-Computer Interfaces
- Regulation of Appetite and Obesity
- Photochromic and Fluorescence Chemistry
- Neuroscience of respiration and sleep
- Dietary Effects on Health
- Neural dynamics and brain function
- Neuroendocrine regulation and behavior
Nagoya University
2019-2024
Japan Society for the Promotion of Science
2020
Japan Science and Technology Agency
2019-2020
Science and Technology Corporation (United States)
2019-2020
Centre de Recherche en Économie et Statistique
2019-2020
Centre for Research in Engineering Surface Technology
2019-2020
A hypothalamic neuronal circuit mediates circadian-regulated wakefulness in mice.
The mammalian central circadian clock, located in the suprachiasmatic nucleus (SCN), coordinates timing of physiology and behavior to local time cues. In SCN, second messengers, such as cAMP Ca
Orexin/hypocretin-producing and melanin-concentrating hormone-producing (MCH) neurons are co-extensive in the hypothalamus project throughout brain to regulate sleep/wakefulness. Ablation of orexin decreases wakefulness results a narcolepsy-like phenotype, whereas ablation MCH increases wakefulness. Since it is unclear how interact sleep/wakefulness, we generated transgenic mice which both could be ablated. Double-ablated exhibited increased decreased rapid eye movement (REM) non-REM (NREM)...
Uninterrupted arousal is important for survival during threatening situations. Activation of orexin/hypocretin neurons implicated in sustained arousal. However, orexin produce and release as well several co-transmitters including dynorphin glutamate. To disambiguate orexin-dependent -independent physiological functions neurons, we generated a novel Orexin-flippase (Flp) knock-in mouse line. Crossing with Flp-reporter or Cre-expressing mice showed gene expression exclusively neurons....
Sleep and wakefulness are regulated by both the homeostatic mechanism circadian clock. In mammals, central clock, suprachiasmatic nucleus, in hypothalamus plays a crucial role timing of physiology behavior. Recently, we found that regulation was transmitted via corticotropin-releasing factor (CRF) neurons paraventricular nucleus to orexin lateral hypothalamus. However, it is still unclear how molecular clock CRF contributes sleep wakefulness. present study, established neuron-specific Bmal1...
In mammals, the central circadian clock is located in suprachiasmatic nucleus (SCN) of hypothalamus, which transmits information to other brain regions and regulates timing sleep wakefulness. Neurons lateral hypothalamus (LH), particularly those producing melanin-concentrating hormone (MCH)- orexin are key regulators Although SCN receives non-photic input from regions, mechanisms functional LH remain poorly understood. Here, we show that MCH peptides influence period within both sexes. When...
Summary Orexin/hypocretin-producing and melanin-concentrating hormone-producing (MCH) neurons are co-extensive in the tuberal hypothalamus project throughout brain to regulate sleep/wakefulness. Ablation of orexin mice decreases wakefulness results a narcolepsy-like phenotype, whereas ablation MCH increases wakefulness. Since it is unclear how interact sleep/wakefulness, we generated conditional transgenic which both could be ablated. Double-ablated exhibited increased decreased rapid eye...
Sleep-stage classification is essential for sleep research. Various automatic judgment programs, including deep learning algorithms using artificial intelligence (AI), have been developed, but limitations with regard to data format compatibility, human interpretability, cost, and technical requirements. We developed a novel program called GI-SleepNet, generative adversarial network (GAN)-assisted image-based staging mice that accurate, versatile, compact, easy use. In this program,...
Abstract Uninterrupted arousal is important for survival during threatening situations. Activation of orexin/hypocretin neurons implicated in sustained arousal. However, orexin produce and release as well several co-transmitters including dynorphin glutamate. Thus, it to disambiguate peptide-dependent -independent physiological functions neurons. To attain this, we generated a novel orexin-flippase (Flp) knock-in (KI) mouse line. Crossing with Flp-reporter or Cre-expressing mice showed gene...
Orexin-producing neurons (orexin neurons) and melanin-concentrating hormone-producing (MCH are exclusively distributed in the lateral hypothalamus project throughout whole brain to regulate sleep/wakefulness. Ablation of orexin mice decreases wakefulness dark period they display a narcolepsy-like phenotype, whereas ablation MCH increases wakefulness. It is unclear how interaction between regulates Here we generated transgenic which both ablated. These double-ablated show increased decreased...
<title>Abstract</title> Sleep-stage classification is essential for sleep research. Various automatic judgment programs including deep learning algorithms using artificial intelligence (AI) have been developed, but with limitations in data format compatibility, human interpretability, cost, and technical requirements. We developed a novel program called GI-SleepNet, generative adversarial network (GAN)-assisted image-based staging mice that accurate, versatile, compact, easy to use. In this...