A5008850254- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Animal Behavior and Reproduction
- Avian ecology and behavior
- Plant and animal studies
- Chromosomal and Genetic Variations
- Reproductive biology and impacts on aquatic species
- Animal Nutrition and Physiology
- Plant Reproductive Biology
- Insect and Pesticide Research
- RNA and protein synthesis mechanisms
- Hypothalamic control of reproductive hormones
- Ovarian function and disorders
- Sexual Differentiation and Disorders
- Genetic and phenotypic traits in livestock
- Genomics and Chromatin Dynamics
- Animal Ecology and Behavior Studies
- RNA Research and Splicing
- Muscle Physiology and Disorders
- Cancer-related molecular mechanisms research
- Bee Products Chemical Analysis
- S100 Proteins and Annexins
Hokkaido University
2017-2024
Hokkaido University of Education
2024
Hokkaido University of Science
2023
Hiroshima University
2021
Fracture Analysis Consultants (United States)
2019
University of Toyama
2016
Shizuoka University
2012-2015
Waseda University
2012
Nagoya University
2007-2010
Obihiro University of Agriculture and Veterinary Medicine
2000-2001
Mammalian sex chromosomes are highly conserved, and is determined by SRY on the Y chromosome. Two exceptional rodent groups in which some species lack a chromosome Sry offer insights into how novel genes can arise replace , leading to turnover. However, intensive study over three decades has failed reveal identity of either these lineages. We here report our discovery male-specific duplication an enhancer Sox9 Amami spiny rat Tokudaia osimensis males females have only single X (XO/XO)...
Abstract Although successful fertilization depends on timely encounters between sperm and egg, the decoupling of mating often confers reproductive advantages to internally fertilizing animals. In several vertebrate groups, postcopulatory viability is prolonged by storage in specialized organs within female tract. birds, ejaculated can be stored a quiescent state oviductal tubules (SSTs), thereby retaining fertilizability for up 15 weeks at body temperature (41 °C); however, mechanism which...
Systems for maintaining the viability of ejaculated sperm in female reproductive tract are widespread among vertebrates and invertebrates. In birds, this storage function is performed by specialized simple tubular invaginations called tubules (SSTs) uterovaginal junction (UVJ) oviduct. Although incidence physiological reasons birds have been reported extensively, mechanisms uptake SSTs, maintenance within control release from SSTs poorly understood. study, we demonstrated that highly...
Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI not successful birds because of the size egg and difficulty mimicking physiological polyspermy that takes place during normal fertilization. Microsurgical 20 or more spermatozoa into an is detrimental its survival. Here, we report a single spermatozoon with small volume extract (SE) components led development birth healthy quail chicks. SE...
Maternal immunoglobulin transfer plays a key role in conferring passive immunity to neonates. blood Y (IgY) avian species is transported newly-hatched chicks two steps: 1) IgY from the maternal circulation yolk of maturing oocytes, 2) deposited embryo via sac membrane. An IgY-Fc receptor, FcRY, involved second step, but mechanism first step still unclear. We determined whether FcRY was also basis for during egg development. Immunohistochemistry revealed that expressed capillary endothelial...
Abstract This study was conducted to investigate the role of a sperm‐borne compound in oocyte activation special reference time when is required by testicular cells during spermatogenesis quail. First, effects microinjection quail sperm extract (SE) and phospholipase Cζ (PLCζ) cRNA into oocytes were assessed observation pronuclear formation cytoplasmic segmentation, respectively. Secondly, round spermatids with or without PLCζ studied development. When injected SE at 0.13 mg protein/ml, both...
Abstract Fertilization is an indispensable step for formation of a zygote in sexual reproduction, leading to species survival. When mating occurs, sperm transported the female reproductive tracts via seminal plasma (SP). SP derived from male accessory sex glands and it plays pivotal roles fertilization animals. However, molecular mechanisms or fluid successful remain unclear. Here, we report that quail cloacal gland (CG) produces prostaglandin F 2α (PGF ) contributes fertilization. PGF , as...
At the time of fertilization, extracellular matrix surrounding avian oocytes, termed perivitelline membrane (pvm), is hydrolyzed by a sperm-borne protease, although actual protease that responsible for digestion pvm remains to be identified. Here, we show evidence ubiquitin-proteasome system functional in fertilization Japanese quail. The activities induction acrosome reaction and binding ZP3 as revealed ligand blotting purified serum ZP1 are similar those ZP1. Western blot analysis use...
Our previous study demonstrated that elongated spermatids and sperm carrying the female-specific W-chromosome of sex-reversed domestic fowl can activate mouse oocyte, but whether they fertilize avian oocyte lead to a developing zygote remains undetermined. A single isolated from semen testis normal rooster hen was microinjected into quail cultured for 20 24 h. Blastoderms were fixed, cleaved, nuclei stained by 4′,6′-diamidino-2-phenylin-dole, developmental stages assessed. In group,...
The present study was conducted to improve the rate and stage of blastoderm embryo development quail oocytes by intracytoplasmic sperm injection (ICSI) with phospholipase Cζ (PLCζ) cRNA. Blastoderm cultured for 24 or 72h were staged under a stereomicroscope. blastoderms stained 4,6-diamidino-2-phenylindole (DAPI) cell division progress. A PLCζ cDNA clone isolated from RT-PCR. 1978bp nucleotide sequence contained an ORF encoding 637 amino acids protein. deduced protein consists EF-hand...
This study was conducted to establish a new method of avian transgenesis by intracytoplasmic sperm injection (ICSI). First, we evaluated the fertilization ability quail oocytes after microinjection Triton X-100 (TX-100)-treated with PLCZ cRNA. The were cultured for 24 h, and blastoderm development examined histological observation. TX-100 treatment induced damage membrane interfered injected sperm. On other hand, when TX-100-treated cRNA simultaneously, 43.5% (10/23) developed into...
DEAD-box helicase 4 (DDX4; also known as vasa) is essential for the proper formation and maintenance of germ cells. Although DDX4 conserved in a variety vertebrates invertebrates, its roles differ between species. This study investigated function chicken embryos by knocking down expression using retroviral vectors that encoded DDX4-targeting microRNAs. was effectively depleted invitro invivo via this approach. Male female gonads DDX4-knockdown contained decreased number primordial cells,...
Abstract Primordial germ cells (PGCs) are essential precursors for sperm and eggs. PGCs highly reliable conserving chicken germplasm producing genetically modified chickens. Currently, efforts to conserve native through research on chickens using CRISPR/Cas9 limited. In this study, we successfully established 289 PGC lines from eight Chinese populations with an 81.6% derivation rate. Furthermore, regenerated Piao derived these by repropagating cryopreserved transplanting them into recipient...
DEAD-box RNA helicase 4 (DDX4) is posited to be a key maternal germ cell factor regulating avian formation. We herein showed that the DDX4 gene product of zygotic genome activation associated with nuclear localization cyclin D1 protein in presumptive primordial cells (PGCs) plays an essential role proliferation PGCs using CRISPR/Cas9 system approach combined vitro fertilization techniques Japanese quail. A proteome analysis also revealed molecular-based differences features early male and...
The present study was conducted to show a profile of mRNA expression sex-differentiation related genes in the duck gonad. Fertile eggs (Cherry-valley) were incubated (hatching at day 28 incubation) and gonads collected days 7, 8, 10, 12 15 incubation after taking photos gonads. Left gonad used for identification genetic sex total RNA extraction. Reverse transcription-polymerase chain reaction (PCR) performed using appropriate forward reverse primers each FOXL2, P450arom, DMRT1, AMH, P450c17,...
Because of the presence sperm storage tubules (SSTs) in utero-vaginal junction (UVJ) oviduct, once ejaculated enter female reproductive tract, they can survive for a prolonged period domestic birds; however, specific mechanisms involved maintenance within SST remain to be elucidated. In this study, we showed that transferrin (TF) and albumin (ALB) are expressed SSTs. When UVJ extracts were subjected size-exclusion column chromatography, obtained fractions extend longevity vitro. LC-MS/MS...
The present study was conducted to investigate (1) the effective day of treatment with Fadrozole, nonsteroidal aromatase inhibitor (AI), during incubation for sex reversal, (2) mRNA expression (P450arom), anti-Müllerian hormone (AMH) and estrogen receptor α (ERα) in quail gonad gonadal structures after AI before hatching (3) effects on growth, left-right asymmetry body weight gain between 1 7 weeks posthatch. Females AI-treated at 0, 2 4 days had symmetrical gonads hatch similar normal...
Abstract Although a rise in intracellular calcium concentration of vertebrate oocytes plays pivotal role for the initiation fertilization or oocyte activation, no study on this subject has been reported birds. This was conducted to relation avian oocytes. First, immediately after quail injected with sperm, it treated strontium chloride as an inducer at doses 0, 2.5, 5, 7.5, 10 mM 4 hr culture medium and followed by 20‐hr culture. Treatment 5 induced blastodermal development 24.2% eggs,...