A5039297032- Brucella: diagnosis, epidemiology, treatment
- Protist diversity and phylogeny
- Aquatic Ecosystems and Phytoplankton Dynamics
- Escherichia coli research studies
- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
- Biochemical and Structural Characterization
- Bacteriophages and microbial interactions
- Burkholderia infections and melioidosis
- Bacterial biofilms and quorum sensing
- COVID-19 Clinical Research Studies
- Evolution and Genetic Dynamics
- Lipid Membrane Structure and Behavior
- Biocrusts and Microbial Ecology
- Galectins and Cancer Biology
- SARS-CoV-2 detection and testing
- Legume Nitrogen Fixing Symbiosis
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Physiological and biochemical adaptations
University of Namur
2012-2023
UCLouvain
2022
Laboratoire de Mathématiques d'Orsay
2012
Several bacterial pathogens have TIR domain-containing proteins that contribute to their pathogenesis. We identified a second TIR-containing protein in Brucella spp. we designated BtpB. show it is potent inhibitor of TLR signaling, probably via MyD88. BtpB novel effector translocated into host cells and interferes with activation dendritic cells. In vivo mouse studies revealed contributing virulence control local inflammatory responses relevance the establishment chronic brucellosis....
Bdelloid rotifers are notorious as a speciose ancient clade comprising only asexual lineages. Thanks to their ability repair highly fragmented DNA, most bdelloid species also withstand complete desiccation and ionizing radiation. Producing well-assembled reference genome is critical step developing an understanding of the effects long-term asexuality DNA breakage on evolution. To this end, we present first high-quality chromosome-level assemblies for Adineta vaga, composed six pairs...
Brucella spp. and Sinorhizobium meliloti are alphaproteobacteria that share not only an intracellular lifestyle in their respective hosts, but also a crucial requirement for cell envelope components timely regulation successful infectious cycle. Here, we report the characterization of melitensis mucR, which encodes zinc finger transcriptional regulator has previously been shown to be involved cellular mouse infections at early time points. MucR modulates surface properties bacteria...
Brucella quorum sensing has been described as an important regulatory system controlling crucial virulence determinants such the VirB type IV secretion and flagellar genes. However, basis of sensing, namely production autoinducers in questioned. Here, we report data obtained from use a genetic tool allowing situ detection long-chain N-acyl-homoserine lactones (AHL) activity at single bacterium level melitensis. These are consistent with intrinsic AHL by B. melitensis low concentration both...
Brucella are facultative intracellular bacteria that cause chronic infections by limiting innate immune recognition. It is currently unknown whether FliC flagellin, the monomeric subunit of flagellar filament, sensed host during infection. Here, we used two mutants melitensis, either lacking or overexpressing to show hinders bacterial replication in vivo. The use cells and mice genetically deficient for different components inflammasomes suggested was a target cytosolic receptor NLRC4 vivo...
Rotifers of the class Bdelloidea are microscopic animals notorious for their long-term persistence in apparent absence sexual reproduction and meiotic recombination. This evolutionary paradox is often counterbalanced by invoking ability to repair environmentally induced genome breakage. By studying dynamics DNA damage response bdelloid species Adineta vaga , we found that it occurs rapidly soma, producing a partially reassembled genome. contrast, germline delayed specific time window...
Bdelloid rotifers are part of the restricted circle multicellular animals that can withstand a wide range genotoxic stresses at any stage their life cycle. In this study, bdelloid rotifer Adineta vaga is used as model to decipher molecular basis extreme tolerance. Proteomic analysis shows specific DNA ligase, different from those usually involved in repair eukaryotes, strongly over-represented upon ionizing radiation. A phylogenetic reveals its orthology prokaryotic ligase E, and horizontal...
Wastewater-based surveillance was conducted by the national public health authority to monitor SARS-CoV-2 circulation in Belgian population. Over 5 million inhabitants representing 45% of population were monitored throughout 42 wastewater treatment plants for 15 months comprising three major virus waves. During entire period, a high correlation observed between daily new COVID-19 cases and concentration corrected rain impact covered size. Three alerting indicators included weekly...
ABSTRACT The several hundreds of species bdelloid rotifers are notorious because they represent an ancient clade comprising only asexual lineages 1 . Moreover, most have the ability to withstand complete desiccation and high doses ionizing radiation, being able repair their DNA after massive genome breakage 2 To better understand impact long-term asexuality on evolution, a telomere-to-tolemere reference assembly is critical 3, 4 Here we present first, quality chromosome-scale for A. vaga...