A5037698048- Organ Transplantation Techniques and Outcomes
- Pancreatic function and diabetes
- Renal Transplantation Outcomes and Treatments
- Organ Donation and Transplantation
- Neurological Complications and Syndromes
- Pluripotent Stem Cells Research
- Diabetes Management and Research
- Complement system in diseases
- CRISPR and Genetic Engineering
- Acute Kidney Injury Research
- Peroxisome Proliferator-Activated Receptors
- Biomedical Ethics and Regulation
- Lipid metabolism and biosynthesis
- Pancreatic and Hepatic Oncology Research
- Epilepsy research and treatment
- Renal and related cancers
- Diabetes and associated disorders
- Enzyme Catalysis and Immobilization
- Liver Disease and Transplantation
- Diabetes Treatment and Management
University of Wisconsin–Madison
2017-2021
Cellular Dynamics International (United States)
2010
Baylor College of Medicine
2008
Induced pluripotent stem cells (iPSCs) hold enormous potential for the development of personalized in vitro disease models, genomic health analyses, and autologous cell therapy. Here we describe generation T lymphocyte-derived iPSCs from small, clinically advantageous volumes non-mobilized peripheral blood. These T-cell derived ("TiPS") retain a normal karyotype genetic identity to donor. They share common characteristics with human embryonic (hESCs) respect morphology,...
Renal transplant outcomes result from a combination of factors. Traditionally, donor factors were summarized by classifying kidneys as extended criteria or standard criteria. In 2014, the nomenclature changed to describe with kidney profile index (KDPI). We aim evaluate relationship between KDPI and delayed graft function (DGF), impact on for both after cardiac death (DCD) brain (DBD).An IRB-approved single-center retrospective chart review was performed January 1999 July 2013. The patients...
The complement system is an essential component of innate immunity and plays a major role in the pathogenesis ischemia-reperfusion injury (IRI). In this study, we investigated impact human C1-inhibitor (C1INH) on early inflammatory response to IRI subsequent progression fibrosis mice. We evaluated structural damage, renal function, acute response, overall survival at 90-days post-injury. Animals receiving C1INH prior reperfusion had significant improvement rate along with superior function...
Delayed graft function (DGF) in renal transplant is associated with reduced survival and increased immunogenicity. The complement-driven inflammatory response after brain death (BD) posttransplant reperfusion injury play significant roles the pathogenesis of DGF. In a nonhuman primate model, we tested complement-blockade BD donors to prevent DGF improve survival. were maintained for 20 hours; kidneys procured stored at 4°C 43-48 hours prior implantation into ABO-compatible, nonsensitized,...
Complement activation in kidney transplantation is implicated the pathogenesis of delayed graft function (DGF). This study evaluated therapeutic efficacy high-dose recombinant human C1 esterase inhibitor (rhC1INH) to prevent DGF a nonhuman primate model after brain death and prolonged cold ischemia.Brain donors underwent 20 h conventional management. Procured kidneys were stored on ice for 44-48 h, then transplanted into ABO-compatible major histocompatibility complex-mismatched recipients....
Background. Brain death (BD)–associated inflammation has been implicated in decreased kidney allograft function and survival, but the underlying mechanisms have not well distinguished from conditions of critical care itself. We developed a clinically translatable model to separate investigate strategies improve donor management care. Methods. Brain-dead (n = 12) sham 5) rhesus macaques were maintained for 20 hours under intensive unit–level conditions. Samples collected immunophenotyping,...
The development of a translatable brain death animal model has significant potential to advance not only transplant research, but also the understanding pathophysiologic changes that occur in and severe traumatic injury. aim this paper is describe rhesus macaque designed simulate average time medical management described human literature.Following approval by Institutional Animal Care Use Committee, was developed. Non-human primates were monitored maintained for 20 hours after induction....
Access to functional high-quality pancreatic human islets is critical advance diabetes research. The Integrated Islet Distribution Program (IIDP), a major source for islet distribution over 15 years, conducted study evaluate the most advantageous times ship postisolation maximize recovery. For evaluation, three experienced IIDP Isolation Centers each provided samples from five isolations, shipping 10,000 equivalents (IEQ) at four different time periods postislet isolation (no 37°C culture...
Background. Kidney after liver transplantation (KALT) is the best therapeutic option for patients with end-stage renal disease orthotopic (OLT). New allocation policies prioritize kidneys to in failure within first year following OLT. There little data on how kidney quality, measured by donor profile index (KDPI), impacts KALT survival outcomes. Methods. The United Network Organ Sharing database was queried adult recipients from 1988 2015 and compared their paired transplant alone (KTA)...