A Gram-stain-positive, facultatively anaerobic, rod-shaped strain, designated SPB1-3 T , was isolated from tree bark. This strain exhibited heterofermentative production of dl -lactic acid glucose. Optimal growth observed at 25–40 °C, pH 4.0–7.0, and in the presence 3% (w/v) NaCl. The cell wall peptidoglycan contained lysine aspartic acid. predominant fatty acids identified were C 16:0 Summed feature 7 (C 19 :1 ω 7c/C 19:1 ω6 c and/or 6c/ 7c/19cy). polar lipid profile included...

Silk sericin (SS), a biocompatible protein derived from silkworms, exhibits valuable properties for medicinal applications, including antioxidant activity and cell growth support. However, their rapid degradation limits practical use. This study introduces silver ions (Ag+) as dual-function cross-linking agent to enhance the structural functional of SS-based hydrogels. The incorporation Ag+ stabilized hydrogel network through dityrosine cross-links coordination bonds with SS amino acid side...

Marine sponges are well-known for their production of bioactive compounds, many which synthesized by associated symbiotic microorganisms. Among these, Actinomycetes particular interest due to ability produce secondary metabolites with antimicrobial and antitumor activities. We aimed investigate the bacterial microbiome tropical marine sponges, an emphasis on diversity distribution Actinomycetes, employing both culture-dependent culture-independent approaches. Five sponge samples (PF01-PF05)...

Two new marine actinobacteria, designated as J2-1T and J2-2T, were isolated from a coral, Favites pentagona, collected Rayong Province, Thailand. The taxonomic positions of the two strains identified based on polyphasic taxonomy. Based morphological characteristics chemotaxonomy, J2-2T members genus Streptomyces Kineosporia, respectively. Strains showed highest 16S rRNA gene sequence similarity to broussonetiae T44T (98.62 %) Kineosporia babensis VN05A0415T (98.08 %), Strain had...

Abstract The highly acid sulfate Rangsit soil series of Rangsit, Pathum-Thani district, Thailand poses a major problem for agriculture in the area. Water hyacinth is naturally occurring weed that can grow aggressively, causing eutrophication and leading to many severe environmental impacts. Here, through pyrolysis process, we convert water biochar use it amendment. We found ratio between biochar, soil, sand suitable cultivation convolvulus be 50 g 400 100 (1:8:2). This mixture improved pH...

A novel lichen-derived actinobacterium, designated Pm04-4 T , was isolated from Pyxine cocoes (Sw.) Nyl. lichen collected Chaiyaphum, Thailand. polyphasic approach used to describe the taxonomic position of strain. The strain had morphological and chemotaxonomic properties similar members genus Actinoplanes . It produced sporangia on substrate mycelia. Meso -diaminopimelic acid, galactose, glucose mannose were detected in whole-cell hydrolysate major menaquinone MK-9(H 4 ). polar lipids...

The novel actinomycete strain PWB-003T, which produced fluostatins B and C antibiotics, was isolated from nearshore sediment collected Panwa Cape, Phuket Province, Thailand. Data the present polyphasic study indicated that PWB-003T represented a member of genus Micromonospora. It single spores on substrate mycelia contained meso-diaminopimelic acid in cell-wall peptidoglycan. Whole-cell hydrolysate ribose, xylose, arabinose, mannose glucose. predominant menaquinone MK-10 (H4). Cellular fatty...

Two actinomycete strains, KK1-2(T) and CPB4-7, were isolated from marine sediments collected in Chumphon province, Thailand. Their taxonomic positions determined using a polyphasic approach. The morphological, cultural chemotaxonomic characteristics of these isolates consistent with the classification strains as representing member genus Streptomyces. They contained LL-diaminopimelic acid their cell wall peptidoglycan; whole-cell sugars ribose glucose. predominant menaquinones MK9-(H6)...

<abstract> <p>Antibiotic resistance is one of the most important global healthcare challenges and responsible for mortality millions people worldwide every year. It a crisis attributed to misuse antibiotics lack new drug development. Actinomycetes constitute group Gram-positive bacteria known their distinctive high guanine-cytosine (G+C) content in genomic DNA. These microorganisms are widely recognized capability generate wide range secondary metabolites with diverse biological...

A novel species of the genus Actinoplanes, strain LDG1-22T, for which we propose name Actinoplanes lichenis sp. nov., was isolated from a lichen sample collected tree bark in Thailand. The taxonomic position has been described based on polyphasic approach. Strain LDG1-22T produced irregular sporangia agar media. It contained meso-diaminopimelic acid cell-wall peptidoglycan. major menaquinone MK-9(H4); polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol,...

The taxonomic position of an actinomycete, strain NR4-ASC07T, isolated from a soil sample collected Sirindhorn peat swamp forest, Narathiwat Province, Thailand, was clarified using polyphasic approach. On the basis morphological and chemotaxonomic characteristics, it classified among members genus Nonomuraea. It produced tightly closed spiral spore chains on aerial mycelium as well forming pseudosporangium. Whole-cell hydrolysates contained meso-diaminopimelic acid, glucose, ribose, madurose...

Two actinobacteria, designated as strain LDG1-01 T and LDG1-06 , were isolated from lichen samples collected in Thailand. Results of morphological characterization, chemotaxonomic studies 16S rRNA gene analysis indicated that both strains members the genus Actinoplanes . MK-9(H 4 ) was found major menaquinone. The fatty acids anteiso-C 15 : 0 iso-C 16 17 Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine phosphatidylinositol observed polar lipids, but differences minor...

The cholesterol-lowering and immunomodulatory effects probiotic properties of 25 lactic acid bacteria (LAB) isolated from fermented fish (pla-paeng-daeng) in Thailand were examined the present study. Based on their phenotypic genetic characteristics, LAB identified as Lactiplantibacillus pentosus (Group I, 6 isolates), argentoratensis II, 1 isolate), Limosilactobacillus fermentum III, 2 Companilactobacillus pabuli IV, 4 farciminis V, 5 futsaii VI, Enterococcus lactis VII, isolate). PD3-1...

A novel actinomycete, strain RCU-197T, was isolated from soil of a peat swamp forest in Rayong Province, Thailand. Using polyphasic approach, the classified genus Streptomyces. It contained ll-diaminopimelic acid cell-wall peptidoglycan. No diagnostic sugars were detected whole-cell hydrolysates and there lack mycolic acids. The major menaquinones MK-9(H6) MK-9(H8). predominant cellular fatty acids iso-C14 : 0, iso-C15 anteiso-C15 0 iso-C16 0. polar lipids profile consisted...

Five actinomycete isolates, CPB1-1T, CPB2-10, BM1-4, CPB3-1 and CPB1-18, belonging to the genus Streptomyces were isolated from marine sediments collected Chumphon Province, Thailand. They produced open loops of warty spore chains on aerial mycelia. ll-Diaminopimelic acid, glucose ribose found in their whole-cell hydrolysates. Polar lipids diphosphatidylglycerol, phosphatidylethanolamine, lysophosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannoside. Menaquinones...

The taxonomic position of the mountain soil actinomycete, strain CYP1-1BT, was clarified by a polyphasic study. produced single spore, or occasionally chain spores, on aerial mycelium. Chemotaxonomic data supported classification CYP1-1BT as representing member genus Actinomadura basis presence meso-diaminopimelic acid in peptidoglycan; galactose, glucose, madurose and ribose whole cell sugars; MK-9(H6), MK-9(H8) MK-9(H4) dominant menaquinones; C16 : 0, 10-methylated C18 0 1ω9c major...

The novel actinomycete, strain CH3-3T, was isolated from mangrove sediment collected Chonburi Province, Thailand. On the basis of its spore morphology and chemotaxonomic characteristics, belonged to genus Micromonospora. It contained meso-diaminopimelic acid, glucose, mannose, xylose, ribose rhamnose in whole-cell hydrolysate, MK-10(H4), MK-10(H6) MK-10(H8) as major menaquinones, iso-C15 : 0, iso-C16 0 iso-C17 cellular fatty acids. blast analysis 16S rRNA gene sequences revealed that showed...

Nile tilapia is one of the most consumed farmed fish in world. The outbreak pathogenic bacterial diseases causes high mortality rates and economic losses farming. Antibiotic administrations are commonly utilized to inhibit prevent infections. However, antibiotics expensive cause serious concerns for antibiotic resistance that can be potentially transferred humans. As an alternative solution, probiotics used infection bacteria fish. In this work, both yeast were isolated from gastrointestinal...

Ten samples of tropical lichens collected from Doi Inthanon, Thailand, were explored for the diversity their bacterial microbiomes through 16S rRNA-based metagenomics analysis. The five predominant lichen-associated bacteria belonged to phyla Proteobacteria (31.84%), Planctomycetota (17.08%), Actinobacteriota (15.37%), Verrucomicrobiota (12.17%), and Acidobacteriota (7.87%). analysis metric showed that Heterodermia contained highest species richness. Within lichens, Ramalina conduplicans...

A new actinobacterial strain, LCR6-01T, was isolated from a lichen sample collected Chiang Rai Province, Thailand. Its taxonomic position determined using polyphasic approach. The strain had morphological characteristics and chemotaxonomic properties identical to those of members the genus Streptomyces . predominant menaquinones were MK-9(H6) MK-9(H8). major fatty acids anteiso-C15 : 0, iso-C15 iso-C16 iso-C14 0 C16 0. polar lipids diphosphatidylglycerol, phosphatidylethanolamine,...

The novel endophytic actinomycete strain PA1-07T was isolated from the stem of Phyllanthus amarus. displayed consistent characteristics members genus Streptomyces. produced short spiral spore chains on aerial mycelia. It grew at pH 5-9, 40 °C and with a maximum 5 % (w/v) NaCl. contained ll-diaminopimelic acid, glucose ribose in whole-cell hydrolysate. major cellular menaquinones were MK-9(H4), MK-9(H6) MK-9(H8), while fatty acids C16 : 0, iso-C14 iso-C16 0 anteiso-C15 0. polar lipids...

Phytochemical screening and Antimicrobial activity of the leaves Memecylon umbellatum burm. F.Subban Murugesan, Annamalai Pannerselvam, Arumugame Chanemougame Tangavelou