A5027610305- Plant nutrient uptake and metabolism
- Advanced Data Storage Technologies
- Plant Molecular Biology Research
- Rice Cultivation and Yield Improvement
Nanjing Agricultural University
2017-2020
The nitrate (NO3-) transporter has been selected as an important gene maker in the process of environmental adoption rice cultivars. In this work, we transferred another native OsNAR2.1 promoter with driving into plants. transgenic lines exogenous pOsNAR2.1:OsNAR2.1 constructs showed enhanced expression level, compared wild type (WT), and
The NO3- transporter plays an important role in rice nitrogen acquisition and nitrogen-use efficiency. Our previous studies have shown that the high affinity systems for nitrate uptake is mediated by a two-component NRT2/NAR2 transport system. In this study, transgenic plants were successfully developed overexpression of OsNAR2.1 alone, OsNRT2.3a alone co-overexpression OsNRT2.3a. our field experiments, lines expressing p35S:OsNAR2.1 or p35S:OsNAR2.1-p35S:OsNRT2.3a constructs exhibited...
The nitrate transport accessory protein OsNAR2 plays a critical role in root-growth responses to and acquisition rice (Oryza sativa). In this study, pull-down assay combined with yeast two-hybrid coimmunoprecipitation analyses revealed that OsNAR2.1 interacts OsNIT1 OsNIT2. Moreover, an vitro nitrilase activity indicated indole-3-acetonitrile (IAN) is hydrolyzed indole-3-acetic acid (IAA) by OsNIT1, the of which was enhanced 3- 4-fold OsNIT2 excess 5- 8-fold OsNAR2.1. Knockout (KO)...
Abstract Nitrate is an essential nitrogen source and serves as a signal to control growth gene expression in plants. In rice, OsNAR2.1 partner of multiple OsNRT2 nitrate transporters for uptake over low high concentration range. Previously, we have reported that −311 bp upstream fragment from the translational start site promoter responsive region. To identify cis -acting DNA elements necessary induced expression, detected beta-glucuronidase (GUS) reporter transgenic rice driven by with...