A5072906005- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Mitochondrial Function and Pathology
- Cellular Mechanics and Interactions
- Genetic Neurodegenerative Diseases
- Advanced Fluorescence Microscopy Techniques
- Protein Structure and Dynamics
- Muscle Physiology and Disorders
- Biotin and Related Studies
- Cardiomyopathy and Myosin Studies
- Photoreceptor and optogenetics research
- Force Microscopy Techniques and Applications
- Aortic Disease and Treatment Approaches
- S100 Proteins and Annexins
- Cardiac Valve Diseases and Treatments
- Cardiovascular Effects of Exercise
- Photosynthetic Processes and Mechanisms
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Infective Endocarditis Diagnosis and Management
- Glioma Diagnosis and Treatment
- Pituitary Gland Disorders and Treatments
- Cardiac tumors and thrombi
- bioluminescence and chemiluminescence research
- Biochemical Acid Research Studies
The University of Texas Southwestern Medical Center
2022
University of Minnesota
2012-2021
Twin Cities Orthopedics
2021
The Ohio State University
2020
University of Minnesota System
2019
University of Minnesota, Duluth
2013-2018
Mayo Clinic in Arizona
2007-2011
WinnMed
2011
Mayo Clinic
2006-2007
Hydrophilic coatings on arterial introducer sheaths reduce the frequency of spasm during cardiac catheterization. Moreover, treatment cerebral artery aneurysms, hydrophilic microcatheters ease their maneuverability, and polymers within embolization coils enhance neointimal fibrosis at aneurysm neck. Foreign-body giant cell reactions can result when a polymer is stripped from its source. CASES 1 AND 2: A 51-year-old man 66-year-old woman underwent coronary angiography via radial artery, using...
Abstract Spinocerebellar ataxia type 5 (SCA5) is a neurodegenerative disease caused by mutations in the cytoskeletal protein β-III-spectrin. Previously, SCA5 mutation resulting leucine-to-proline substitution (L253P) actin-binding domain (ABD) was shown to cause 1000-fold increase affinity. However, structural basis for this unknown. Here, we report 6.9 Å cryo-EM structure of F-actin complexed with L253P ABD. This structure, along co-sedimentation and pulsed-EPR measurements, demonstrates...
Synaptotagmin I (Syt I) is a vesicle-localized protein implicated in sensing the calcium influx that triggers fast synchronous release of neurotransmitter. How Syt utilizes its two C2 domains to integrate signals and mediate neurotransmission has continued be controversial area research, though prevalent hypotheses favor independent function. Using differential scanning calorimetry fluorescence lifetime spectroscopy thermodynamic denaturation approach, we tested an alternative hypothesis...
Synaptotagmin I (Syt I) is a vesicle-localized integral membrane protein that senses the calcium ion (Ca2+) influx to trigger fast synchronous release of neurotransmitter. How cytosolic domains Syt allosterically communicate propagate Ca2+ binding signal throughout not well understood. In particular, it unclear whether intrinsically disordered region (IDR) between I's transmembrane helix and first C2 domain (C2A) plays an important role in allosteric modulation binding. Moreover, structural...
Cardiac fibromas are the 2nd most common benign cardiac tumor occurring in children and bear a striking morphologic resemblance to soft tissue or desmoid fibromatosis. Since activating mutations beta-catenin fibromatosis as well other spindle cell proliferations, aim of our study was determine if such could be identified fibroma. Nine from patients with surgical resection were examined for by immunoperoxidase staining protein DNA sequencing region exon 3 gene, where relatively conserved have...
Synaptotagmin I (Syt I) is the calcium ion sensor for regulated release of neurotransmitter. How Syt mediates this cellular event has been a question extensive study decades and yet, clear understanding protein’s diverse functionality remained elusive. Using tools thermodynamics, we have identified two intrinsic properties that may account I’s functional plasticity: marginal stability negative coupling. These potential to provide great conformational flexibility suggest plasticity stems in...
As part of an upper level undergraduate developmental biology course at the University Minnesota Duluth, we developed a unit in which students carried out original research as cooperative class project. Students had opportunity to gain experience scientific method from experimental design all way through preparation publication on their that included text, figures, and tables. This kind inquiry-based learning has been shown have many benefits for students, including increased long-term...
Sudden unexpected death due to hemopericardium generally has 2 main causes, rupture of the ascending aorta or a transmural myocardial infarct. We report an unusual cause fatal in 56-year-old woman that occurred setting motor vehicle mishap.At autopsy, 300 mL blood were present pericardial sac, previously undetected congenital aortic sinus aneurysm. The low velocity automobile at time impact with fence, and absence vehicle-related trauma, led conclusion aneurysm caused mishap, rather than...