A5073017417- Photoreceptor and optogenetics research
- Bacillus and Francisella bacterial research
- RNA and protein synthesis mechanisms
- Photochromic and Fluorescence Chemistry
- Biomedical and Engineering Education
- Biotin and Related Studies
- Insect and Pesticide Research
- Protein Structure and Dynamics
- Pharmaceutical and Antibiotic Environmental Impacts
- Viral Infections and Immunology Research
- Neurobiology and Insect Physiology Research
- Law, AI, and Intellectual Property
- Ethics in Clinical Research
- bioluminescence and chemiluminescence research
- Rabies epidemiology and control
- Computational Drug Discovery Methods
- Viral Infections and Outbreaks Research
- Microplastics and Plastic Pollution
- Mass Spectrometry Techniques and Applications
- Zoonotic diseases and public health
- Effects and risks of endocrine disrupting chemicals
- Entomopathogenic Microorganisms in Pest Control
- Genomics and Chromatin Dynamics
- Ethics and Social Impacts of AI
- Animal testing and alternatives
Gryphon Scientific (United States)
2017-2024
University of California, San Francisco
2008-2019
Sandia National Laboratories California
2018
QB3
2013-2016
We used replica exchange molecular dynamics (REMD) simulations to evaluate four different AMBER force fields and three implicit solvent models. Our aim was determine if these physics-based models captured the correct secondary structures of two α-helical β-peptides: 14-mer EK helix Baldwin co-workers, C-terminal ribonuclease, 16-mer hairpin protein G, trpzip2 miniprotein. The gave results, but generally we found that ff96 plus model Onufriev, Bashford, Case reasonable structures, is fairly...
Sense and respond Many signaling pathways start with cellular proteins sensing responding to small molecules. Despite advances in protein design, creating a protein-based sense-and-respond system remains challenging. Glasgow et al. designed binding sites at the interface of heterodimers (see Perspective by Chica). By fusing each monomer one half split reporter, they linked ligand-driven dimerization reporter output. The computational design strategy provides generalizable approach create...
There is a growing interest in engineering proteins whose function can be controlled with the spatial and temporal precision of light. Here, we present novel example functional light-triggered switch Ca-dependent cell-cell adhesion protein E-cadherin, created using mechanism-based design strategy. We report an 18-fold change apparent Ca(2+) binding affinity upon illumination. Our results include detailed examination switching via linked changes cadherin dimerization. This opens avenues...
Our firm conducted a risk/benefit assessment of "gain-of-function" research, as part the deliberative process following U.S. moratorium on research (U.S. Department Health and Human Services, Government Gain-of-Function Deliberative Process Research Funding Pause Selected Involving Influenza, MERS, SARS Viruses, 2014). Due to significant missing but theoretically acquirable data, our biosafety faced limitations, we were forced provide relative, instead absolute, measure risk (Gryphon...
In this paper we provide a proof of principle new method for addressing the ethics autonomous vehicles (AVs), <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Data-Theories Method</i> , in which vehicle crash data is combined with philosophical ethical theory to guide action AV algorithm design. We use model three scenarios an exposed risk on road, and determine possible actions AV. then examine how different perspectives agent partiality, or...
ABSTRACT Sensing and responding to signals is a fundamental ability of living systems, but despite remarkable progress in computational design new protein structures, there no general approach for engineering arbitrary sensors. Here we describe generalizable strategy designing sensor/actuator proteins by building binding sites de novo into heterodimeric protein-protein interfaces coupling ligand sensing modular actuation via split reporters. Using this approach, designed sensors that respond...
Quantitative data informing biosafety practices have long been lacking. In this study, we describe the conduct of first large-scale investigation into human reliability in life sciences laboratory to estimate an error rate during routine biological experiments. To generate these critical data, conducted two sets experiments: blinded experiments clinical laboratories Brazil, Jordan, and Tunisia, volunteer training U.S. universities. experiments, GloGerm was used indicate where spills occurred...
The rapid increase in the power of life sciences has not been accompanied by a proportionate sophistication biorisk management. Through conversations with thought leaders biosafety and biosecurity, we have identified 19 concepts that are critical for management to continue ensure responsible safe conduct future. Our work is meant be comprehensive list, but rather collection topics hope will spark dialogue policy, research, communities.