Based on the s-d interaction model for dilute magnetic alloys we have calculated scattering probability of conduction electrons to second Born approximation. Because dynamical character localized spin system, Pauli principle should be taken into account in intermediate states order terms. Thus effect Fermi sphere is involved and gives rise a singular term resistivity which involves c log T as factor, where concentration impurity atoms. When combined with lattice resistivity, this resistance...

A neuropathological hallmark of Alzheimer disease (AD) is a widespread amyloid deposition. We analyzed the entire amino acid sequences in an preparation and found, addition to major beta/A4-protein (A beta) fragment, two unknown peptides. raised antibodies against synthetic peptides using subsequences these These immunostained neuritic diffuse plaques as well vascular amyloid. Electron microscopic analysis demonstrated that immunostaining was localized on fibrils. have isolated apparently...

Biochern.188,[535][536][537][538][539][540], not only 40-kDa protein but also 72-kDa were detected on immunoblot analysis of porcine spleen homogenate.In splenocytes preparation, the antibodies could immunoprecipitate protein-tyrosine kinase activity detect even immunoblot.After incubation crude homogenate at 37 "C with or without various protease inhibitors, revealed proteolytic breakdown to fragment.Next, using oligonucleotides designed according partially sequenced information as a probe,...

Hepatocyte growth factor (HGF) is a potent mitogen for parenchymal liver cells, epithelial and endothelial cells may play an important role in regeneration following hepatic injury.HGF homologous to plasminogen first synthesized secreted as inactive single-chain precursor then activated heterodimeric form by endoproteolytic processing.Recently, novel serine protease responsible this processing (HGF activator) has been purified from fetal bovine serum (Shimomura, T., Ochiai, M., Kondo, J.,...

Complementary DNAs encoding rat long-chain acyl-CoA synthetase have been isolated. The cDNAs were identified using synthetic oligonucleotide probes based on partial amino acid sequences of lysyl endopeptidase peptides the purified enzyme. Rat is predicted to contain 699 residues and a calculated molecular weight 78,177. Significant sequence similarity was found between parts firefly luciferase. Based reaction mechanisms two enzymes, we propose function for similar region. mRNA expressed in...

We have purified a novel GTP-binding protein (G protein) with Mr of about 24,000 to homogeneity from bovine brain membranes (Kikuchi, A., Yamashita, T., Kawata, M., Yamamoto, K., Ikeda, Tanimoto, and Takai, Y. (1988) J. Biol. Chem. 263, 2897-2904). In the present studies, we isolated sequenced cDNA this G library using oligonucleotide probes designed partial amino acid sequences. The has an open reading frame encoding 220 acids calculated 24,954. This is designated as smg-25A (smg p25A)....

Hepatocyte growth factor activator (HGF activator) is a serine protease which converts single-chain HGF to the active two-chain form. purified from human serum has molecular mass of 34 kDa and consists two chains held together by disulfide bond. The nucleotide sequence cDNA shows that derived COOH-terminal region precursor 655 amino acids proteolytic cleavage bonds between Arg372 Val373 Arg407 Ile408 multiple domains homologous those observed in blood coagulation XII. In this study, we...

In the present studies, we have purified a novel small Mr GTP-binding protein, designated as smg p21, to near homogeneity from bovine brain crude membranes, isolated complementary DNA (cDNA) of this protein cDNA library, determined complete nucleotide and deduced amino acid sequences, characterized kinetic properties. The p21 has an open reading frame encoding 184 acids with calculated 20,987. is estimated be about 22,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Homology...

We investigate the ordinary and exchange scattering of conduction electrons by magnetic impurities. find that for thermodynamic properties effect is entirely absorbed into an effective interaction $J$ ${cos}^{2}\ensuremath{\eta}$, where $\ensuremath{\eta}$ phase shift due to alone. The resistivity calculated using Nagaoka-Hamann scheme. $n\mathrm{th}$-order leading logarithmic term ($\ensuremath{\sim}{J}^{n+2}{log}^{n}T$ in absence scattering) multiplied ${(cos\ensuremath{\eta})}^{2n+4}$...

We recently purified to near homogeneity a novel type of regulatory protein for smg p25A, ras p21-like GTP-binding protein, from bovine brain cytosol. This named p25A GDP dissociation inhibitor (GDI), regulates the GDP-GTP exchange reaction by inhibiting and subsequent binding GTP it. In present studies, we isolated sequenced cDNA GDI library using an oligonucleotide probe designed partial amino acid sequence GDI. The has open reading frame that encodes 447 acids with calculated Mr 50,565....

Superexchange interaction in the MnO crystal is studied by using Heitler-London picture including overlap. Mechanisms proposed Slater, Kramers and Anderson, Goodenough are developed on same basis. The energy difference between antiparallel spin state parallel computed numerically case of simple four-electron problem.

Mammalian hibernation is a unique physiological adaptation that allows the sustainment of life under extremely low body temperatures. In chipmunk, we found four proteins related specifically to hibernation. These started diminish in concentration blood before and disappeared during reappeared as ceased remained nonhibernation. The complete or partial amino acid sequences showed three (27-, 25-, 20-kDa) were previously unknown, whereas another (55-kDa) highly homologous with alpha...

Abstract We have determined that there are at least six GTP-binding proteins (G proteins) with Mr values between 20,000 and 25,000 in the crude membrane fraction of bovine brain purified one them a about 24,000 (24K G) to near homogeneity (Kikuchi, A., Yamashita, T., Kawata, M., Yamamoto, K., Ikeda, Tanimoto, Takai, Y. (1988) J. Biol. Chem. 263, 2897-2904). In this study, we another G protein (20K characterized it. 20K bound maximally 1.0 mol [35S]guanosine 5'-(3-O-thio)triphosphate (GTP...

The receptor for atrial natriuretic peptide (ANP) was purified to apparent homogeneity from bovine lung by a combination of detergent extraction, ammonium sulfate precipitation, and affinity chromatography on ANP-Affi-Gel 10. Mr the is about 140,000 as determined sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After reduction, protein migrated single band with an near 70,000. NH2-terminal sequence analysis material revealed only one sequence, indicating that ANP composed two...

phoS is the structural gene for phosphate-binding protein, which localized in periplasm and involved active transport of phosphate Escherichia coli. It also a negative regulatory pho regulon, expression inducible by starvation. The complete nucleotide sequence was determined method Maxam Gilbert (A. M. W. Gilbert, Methods Enzymol. 65:499-560, 1980). amino acid sequences at termini pre-PhoS PhoS proteins carboxy terminus protein were using purified proteins. Furthermore, enzymatically...

PERIOD1 (PER1) is a clock gene. We examined the effect of knockdown PER1 on apoptosis in pancreatic cancer (MIA PaCa-2 and PANC-1) hepatocellular carcinoma (HepG2) cells. Transfection siRNA against into these cells increased cleaved forms caspases poly-ADP-ribose-polymerase induced all three cell lines. In two lines, resulted upregulation Bax downregulation Bcl-2. Expression p53 was not altered lines containing mutated p53, but upregulated HepG2 wild-type p53. Cell proliferation MIA...

There are few demonstrated examples of phase transitions that may be driven directly by terahertz frequency electric fields, and those known require field strengths exceeding 1 MV cm-1. Here we report a non-equilibrium transition weak (≪1 V cm-1), continuous-wave field. The system consists room temperature caesium vapour under continuous optical excitation to high-lying Rydberg state, which is resonantly coupled nearby level the We use simple model understand underlying physical behaviour,...

We observe and characterize intrinsic optical bistability in a dilute Rydberg vapor. The is characterized by sharp jumps between states of low high occupancy with jump-up -down positions displaying hysteresis depending on the direction which control parameter changed. find that shift frequency jump point scales fourth power principal quantum number. Also, width window increases increasing number, before reaching peak then closing again. experimental results are consistent predictions from...

In mammals, hibernation is expressed by only a limited number of species, and the molecular mechanisms underlying are not well understood. Recently, we have found plasma proteins which disappear from blood specifically during in mammalian hibernator, chipmunk. Here, report cDNA cloning these chipmunk hibernation-related proteins, HP-20, -25, -27, analyses their expression. All three contain collagen-like domain near N terminus highly homologous to each other. Their mRNAs were detected liver...