A5058741024- Toxin Mechanisms and Immunotoxins
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Radiopharmaceutical Chemistry and Applications
- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Biochemical and Molecular Research
- Organometallic Complex Synthesis and Catalysis
- Galectins and Cancer Biology
- Photosynthetic Processes and Mechanisms
- Bioactive Compounds and Antitumor Agents
- Chemical Reactions and Isotopes
- Metabolism, Diabetes, and Cancer
- Transgenic Plants and Applications
- Hemoglobin structure and function
- Biochemical and Structural Characterization
- Carbohydrate Chemistry and Synthesis
- Advanced Proteomics Techniques and Applications
- Biotin and Related Studies
- Synthesis and Biological Evaluation
- Organometallic Compounds Synthesis and Characterization
National Academy of Sciences of Belarus
2014-2024
Institute of Bioorganic Chemistry
2012-2024
A nanosecond laser near-infrared spectrometer was used to study singlet oxygen (1O2) emission in a protein matrix. Myoglobin which the intact heme is substituted by Zn-protoporphyrin IX (ZnPP) employed. Every collision of ground state molecular with ZnPP excited triplet results 1O2 generation within The quantum yield found be equal 0.9 ± 0.1. On average, six from every 10 molecules succeed escaping matrix into solvent. kinetic model for and subsequent deactivation introduced discussed. Rate...
We describe an experimental approach for direct molecular fishing of prey protein on the surface two types paramagnetic particles (PMP) having different size and composition. Human microsomal cytochrome b(5) (b(5)) its known partner human P450 3A5 (CYP3A5) were used as bait proteins, respectively. For assessing level unspecific binding background α-fetoprotein (aFP) was used. SPR measurements applied quantitative analysis trapped proteins (CYP3A5 aFP) after PMP. It shown that described...
Oligopeptidase B (OpB) is a two-domain, trypsin-like serine peptidase belonging to the S9 prolyloligopeptidase (POP) family. Two domains are linked by hinge region that participates in transition of enzyme between two major states—closed and open—in which residues catalytic triad located close each other separated, respectively. In this study, we described, for first time, structure OpB from bacteria obtained an Serratia proteomaculans with modified (PSPmod). PSPmod was crystallized...
The crystal structure of bacterial oligopeptidase B from Serratia proteamaculans (SpOpB) in complex with a chloromethyl ketone inhibitor was determined at 2.2 Å resolution. SpOpB crystallized closed (catalytically active) conformation. A single molecule bound simultaneously to the catalytic residues S532 and H652 mimicked tetrahedral intermediate reaction. comparative analysis obtained OpB Trypanosoma brucei (TbOpB) conformation showed that both enzymes, stabilization D-loop (carrying D)...
Human InsR, IGF1R, and IRR receptor tyrosine kinases (RTK) of the insulin subfamily play an important role in signaling pathways for a wide range physiological processes are directly associated with many pathologies, including neurodegenerative diseases. The disulfide-linked dimeric structure these receptors is unique among RTKs. Sharing high sequence homology, differ dramatically their localization, expression, functions. In this work, using high-resolution NMR spectroscopy supported by...
The search of a putative physiological electron acceptor for thiocyanate dehydrogenase (TcDH) newly discovered in the thiocyanate-oxidizing bacteria Thioalkalivibrio paradoxus revealed an unusually large, single-heme cytochrome c (CytC552), which was co-purified with TcDH from periplasm. Recombinant CytC552, produced Escherichia coli as mature protein without signal peptide, has spectral properties similar to endogenous and serves vitro TcDH-catalyzed reaction. CytC552 structure determined...
Oligopeptidase B (OPB) is the least studied group from prolyl oligopeptidase family. OPBs are found in bacteria and parasitic protozoa represent pathogenesis factors of corresponding infections. consist two domains connected by a hinge region have characteristics conformational dynamics, which include types movements: bridging/separation α/β-hydrolase catalytic β-propeller-regulatory movement loop carrying histidine, regulates an assembly/disassembly triad. In this work, elucidation...
An analysis of theoretical model studies photochromic systems with reversible fluorescence modulation based on polymer nanospheres containing CdSe/ZnS semiconductor quantum dots (QDs), and diarylethene DAE2 molecules is presented. Based the known relations theory Förster resonance energy transfer (FRET), a constructed for efficiency QD E(r) caused by transformations located near them due to FRET mechanism. The boundaries optimal values parameters that affect are determined. given some...