A5080282379- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Axon Guidance and Neuronal Signaling
- RNA Interference and Gene Delivery
- Spinal Cord Injury Research
- Pluripotent Stem Cells Research
- Signaling Pathways in Disease
- Nuclear Receptors and Signaling
- Receptor Mechanisms and Signaling
- Neuroscience and Neural Engineering
- Autophagy in Disease and Therapy
- Angiogenesis and VEGF in Cancer
- Pain Mechanisms and Treatments
- Stress Responses and Cortisol
- Zebrafish Biomedical Research Applications
- 3D Printing in Biomedical Research
- Anesthesia and Neurotoxicity Research
- Gastrointestinal motility and disorders
- Cholinesterase and Neurodegenerative Diseases
- RNA regulation and disease
- Histone Deacetylase Inhibitors Research
- Neuroendocrine Tumor Research Advances
Rutgers, The State University of New Jersey
2011-2022
Johnson University
2003-2021
Cornell University
1984-1994
The University of Texas MD Anderson Cancer Center
1994
Rockefeller University
1993
Karolinska Institutet
1978-1993
NewYork–Presbyterian Hospital
1990-1991
University Medical Center
1988
Columbia University
1976-1980
Albert Einstein College of Medicine
1977-1980
Although neurotrophins are primarily associated with long-term effects on neuronal survival and differentiation, recent studies have shown that acute changes in synaptic transmission can also be produced. In the hippocampus, an area critically involved learning memory, we found brain-derived neurotrophic factor (BDNF) rapidly enhanced efficacy through a previously unreported mechanism--increased postsynaptic responsiveness via phosphorylation-dependent pathway. Within minutes of BDNF...
We have examined the effect of trophic protein, nerve growth factor (NGF), on organotypic cultures fetal rat striatum. Treatment with NGF for 10-11 days resulted in a 5- to 12-fold increase specific activity cholinergic enzyme choline acetyltransferase (CAT; EC 2.3.1.6). dose-dependent fashion. This was not elicited by insulin, ferritin, or cytochrome c, proteins similar structure physicochemical properties NGF. The CAT specifically blocked anti-NGF antiserum, whereas treatment antiserum...
It is well established that BDNF may enhance oligodendrocyte differentiation following a demyelinating lesion, however, the endogenous sources of be harnessed to reverse deficits associated with such lesions are poorly defined. Here, we investigate roles astrocytes in synthesizing and releasing BDNF. These cells known express injury <i>in vivo</i>. In culture, they increase synthesis release response glutamate metabotropic stimulation. Following cuprizone-elicited demyelination mice, contain...
Contrary to long-held assumptions, recent work indicates that neurons may profoundly change transmitter status during development and maturity. For example, sympathetic neurons, classically regarded as exclusively noradrenergic or cholinergic, can also express putative peptide transmitters such substance P. This neuronal plasticity is directly related membrane depolarization sodium ion influx. The same molecular mechanisms plastic responses occur in mature well developing neurons. Further,...
Previous work in culture has shown that basal forebrain (BF) oligodendrocyte (OLG) lineage cells respond to BDNF by increasing DNA synthesis and differentiation. Further, the BF vivo , reduced levels of as seen +/− mice result numbers NG2+ deficits myelin proteins throughout development adult, suggesting impacts proliferating population OLGs well differentiation . In this study, investigate roles may play repair a demyelinating lesion, cuprizone model was used corpus callosum examined....
The process of oligodendrogenesis has been relatively well delineated in the rodent brain. However, it remains unknown whether analogous developmental processes are manifested human Here we report forebrain organoids, generated by using OLIG2-GFP knockin pluripotent stem cell (hPSC) reporter lines. OLIG2/GFP exhibits distinct temporal expression patterns ventral organoids (VFOs) versus dorsal (DFOs). Interestingly, can be induced both VFOs and DFOs after neuronal maturation. Assembling to...
Glial cell line-derived neurotrophic factor (GDNF) promotes survival of midbrain dopaminergic neurons and motoneurons. Expression GDNF mRNA in cerebellum raises the possibility that cells within this structure might also respond to GDNF. To examine potential trophic activities GDNF, dissociated cultures gestational day 18 rat were grown for < or = 21 days presence factor. increased Purkinje number without affecting overall glial cells. A maximal response (50% above control) was elicited...
The hippocampus and septum play central roles in one of the most important spheres brain function: learning memory. Although their topographic connections have been known for two decades topography may be critical cognitive functions, basis hippocamposeptal projection is unknown. We now report first time that Elf-1, a membrane-bound eph family ligand, candidate molecular tag genesis projection. Elf-1 expressed an increasing gradient from dorsal to ventral septum. Furthermore, selectively...
Traditionally, the primary function of oligodendrocytes (OLGs) in CNS has been considered to be myelination. Here, we investigated whether OLGs may play a trophic role, particularly during development. Neurotrophin expression was assessed postnatal day 7 basal forebrain (BF) OLGs, using situ hybridization and detection myelin basic protein. Nerve growth factor, brain-derived neurotrophic factor (BDNF) neurotrophin-3 (NT-3) mRNAs were revealed vivo culture. To determine support nearby...
Abstract Previous work has indicated that BDNF increases the differentiation of basal forebrain (BF) oligodendrocytes (OLGs) in culture through mediation trkB and MAPK pathway (Du et al. [ 2006a , b ] Mol. Cell. Neurosci. 31:366–375; J. Res. 84:1692–1702). In present work, effects on BF OLG progenitor cells (OPCs) were examined. increased DNA synthesis OPCs, as assessed by thymidine bromodeoxyuridine incorporation. Effects mediated receptor not p75 receptor, shown inhibitors block...
Abstract Previous work indicated that brain‐derived neurotrophic factor (BDNF), through the trkB receptor, increases DNA synthesis in oligodendrocyte (OLG) progenitor cells (OPCs) and differentiation of postmitotic OLGs basal forebrain (BF). In present studies, BDNF knockout animals were used to investigate BDNF's effects on OLG lineage (OLCs) vivo . OLCs BF found express suggesting they are responsive BDNF. Immunohistochemistry using NG2 CC1 antibodies was utilized examine numbers NG2+ OPCs...
A key neurotrophin responsible for the survival and function of basal forebrain (BF) cholinergic neurons is brain-derived neurotrophic factor (BDNF). number studies now indicate that a source this may be BF astrocytes. This study was designed to define role BF-astrocyte-derived BDNF on neurons. Moreover, it investigated regulatory events modulate content release. In initial work derived from BF-astrocyte-conditioned medium (ACM) found increase both numbers acetylcholinesterase (AChE+)...
A specific antibody to tryptophan hydroxylase [L-tryptophan, tetrahydropteridine:oxygen oxidoreductase (5-hydroxylating), EC 1.14.16.4] has been used localize the enzyme immunohistochemically in neurons of mammalian gut. The was found perikarya intestinal mice, rats, and guinea pigs. Neurons containing survived for up 3 weeks organotypic tissue culture were intrinsic These are probably serotonergic first such be peripheral nervous system.