A5022953205- Cardiac Fibrosis and Remodeling
- Bone and Dental Protein Studies
- Tissue Engineering and Regenerative Medicine
- Hemoglobinopathies and Related Disorders
- Epigenetics and DNA Methylation
- Signaling Pathways in Disease
- Reproductive Biology and Fertility
- Mitochondrial Function and Pathology
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Animal Genetics and Reproduction
- Cardiac Ischemia and Reperfusion
- Ubiquitin and proteasome pathways
- Pluripotent Stem Cells Research
- Receptor Mechanisms and Signaling
- Peptidase Inhibition and Analysis
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- Genetic and phenotypic traits in livestock
- Cell death mechanisms and regulation
- Antibiotic Resistance in Bacteria
- Atherosclerosis and Cardiovascular Diseases
- Prenatal Screening and Diagnostics
- Autophagy in Disease and Therapy
- Cancer-related gene regulation
East Tennessee State University
2015-2024
Fort Valley State University
2012-2024
Indian Institute of Management Rohtak
2024
Jaipur Golden Hospital
2014
James H. Quillen VA Medical Center
2003-2013
Jubilant Life Sciences (India)
2013
Jesse Brown VA Medical Center
2004-2010
University of Illinois Chicago
2004-2010
Indian Veterinary Research Institute
2010
University of Illinois Urbana-Champaign
2006
To identify regions of the mitochondrial genome that potentially could specify "Polima" (pol) cytoplasmic male sterility (CMS) Brassica napus, transcripts 14 genes from nap (male fertile), pol sterile), and nuclear fertility-restored cytoplasm plants were analyzed. Transcriptional differences among these detected only with ATPase subunit 6 (atp6) gene. Structural analysis atp6 gene DNAs showed rearrangements in occurring upstream have generated a chimeric 224-codon open reading frame,...
Matrix metalloproteinases (MMPs), a family of endoproteinases, are implicated in cardiac remodeling. Interleukin-1β (IL-1β), which is increased the heart following myocardial infarction, increases expression and activity MMP-2 (gelatinase A) -9 B) fibroblasts. Previously, we have shown that IL-1β activates ERK1/2, JNKs, protein kinase C (PKC). However, signaling pathways involved regulation not yet well understood. Using adult rat fibroblasts, show inhibition ERK1/2 JNKs inhibits...
Osteopontin (OPN) expression increases in the heart during hypertrophy and failure. Here, we studied role of OPN pressure overload–induced analyzed signaling pathways involved hypertrophy. Aortic banding (AB) was performed a group wild-type (WT) knockout (KO) mice to induce overload. Left ventricular (LV) structural functional remodeling 1 month after AB. AB increased β1 integrin (a receptor for OPN) protein WT-AB group. Hypertrophic response as measured by weight-to-body weight ratio...
Matrix metalloproteinases (MMPs), a family of extracellular endopeptidases, are implicated in angiogenesis because their ability to selectively degrade components the matrix. Interleukin-1β (IL-1β), increased heart post-myocardial infarction (post-MI), plays protective role pathophysiology left ventricular (LV) remodeling following MI. Here we studied expression various angiogenic genes affected by IL-1β cardiac microvascular endothelial cells (CMECs) and investigated signaling pathways...
Sympathetic nerve activity increases in the heart during cardiac failure. Here, we hypothesized that beta1 integrins play a protective role chronic beta-adrenergic receptor-stimulated myocyte apoptosis and L-isoproterenol (iso; 400 microg/kg per hour) was infused group of wild-type (WT) integrin heterozygous knockout (hKO) mice. Left ventricular structural functional remodeling studied at 7 28 days iso-infusion. Western blot analysis demonstrated reduced levels myocardium hKO-sham....
Osteopontin, also known as cytokine Eta-1, plays an important role in postmyocardial infarction remodeling by regulating collagen accumulation. Aldosterone promotes synthesis and structural of the heart. The osteopontin aldosterone-induced fibrosis myocardial is unknown. Osteopontin expression left ventricular functional were determined wild-type knockout mice after aldosterone infusion.Immunohistochemical analyses showed increased interstitial protein ventricle 7 days infusion. After 4...
Increased osteopontin (OPN) expression associates with increased myocyte apoptosis and myocardial dysfunction. The objective of this study was to identify the receptor for OPN get insight into mechanism by which induces cardiac apoptosis. Adult rat ventricular myocytes (ARVMs) transgenic mice expressing in a myocyte-specific manner were used vitro vivo studies. Treatment purified (20 nM) protein or adenoviral-mediated induced ARVMs. co-immunoprecipitated CD44 receptors, not β1 β3 integrins....
Abstract Osteopontin (OPN), also called cytokine Eta‐1, expressed in the myocardium co‐incident with heart failure plays an important role post myocardial infarction (MI) remodeling by promoting collagen synthesis and accumulation. Angiotensin II (Ang II) inflammatory cytokines are increased following MI. We studied involvement of mitogen‐activated protein kinases (ERK1/2, JNKs, p38 kinase) reactive oxygen species (ROS) Ang II‐ cytokine‐induced OPN gene expression adult rat cardiac...
We have shown that osteopontin (OPN), an extracellular matrix protein, plays important role in post myocardial infarction (MI) remodeling by promoting collagen synthesis and accumulation. Interleukin-1beta (IL-1beta), increased the heart following MI, increases metalloproteinase (MMP) activity cardiac fibroblasts vitro. Here, we show OPN alone has no effect on MMP or expression. However, it reduces IL-1beta-stimulated expression adult rat fibroblasts. Pretreatment with bovine serum albumin...
Abstract Previous studies have shown that the mitochondrial orf224/atp6 gene region is correlated with Polima (pol) cytoplasmic male sterility (CMS) of Brassica nupus. We now extend this correlation by showing effects nuclear fertility restoration on transcripts cosegregate pol restorer Rfp1 in genetic crosses. also show, however, recessive rpf1 allele, or a very tightly linked gene, acts as dominant designated Mmt (modifier transcripts), controlling presence additional smaller nad4 and...
This study was undertaken to assess the spectrum of drug resistance prevalent in Escherichia coli isolates from human and animal populations Northern India. Three hundred two isolated various infections humans (47 diarrhoea; 101 urinary tract infection) veterinary nnfmnis (17 poultry septicaemia; 75 bovine 14 ovine diarrhoea 48 equine metritis) were studied for their susceptibility ampicillin, cephaloridine, amoxycillin, cloxacillin, oxytetracycline, doxycycline, chloramphenicol, gentamicin,...
Stimulation of β-adrenergic receptors (β-AR) induces apoptosis in adult rat ventricular myocytes (ARVMs) via the JNK-dependent activation mitochondrial death pathway. Recently, we have shown that inhibition matrix metalloproteinase-2 (MMP-2) inhibits β-AR-stimulated and apoptotic effects MMP-2 are possibly mediated its interaction with β 1 integrins. Herein tested hypothesis impairs integrin-mediated survival signals, such as focal adhesion kinase (FAK), activates Inhibition using SB3CT, a...
Changes in the synthesis and activity of matrix metalloproteinases (MMPs) their inhibitors (TIMPs) are associated with myocardial remodeling. Here we measured expression MMPs TIMPs, tested hypothesis that increased MMP plays a proapoptotic role beta-adrenergic receptor (beta-AR)-stimulated apoptosis adult rat ventricular myocytes (ARVMs). beta-AR stimulation (isoproterenol, 24 h) mRNA levels MMP-2 TIMP-1 while it decreased TIMP-2 as analyzed by real-time PCR. Western blot analysis,...
Aimsβ-Adrenergic receptor (β-AR) stimulation induces apoptosis in adult rat ventricular myocytes (ARVMs) via the activation of glycogen synthase kinase-3β (GSK-3β) and mitochondrial pathways. However, β-AR only a fraction (∼15–20%) ARVMs. We hypothesized that ARVMs may secrete/release survival factor(s) which protects 80–85% cells from apoptosis.