A5089675819- Genetic Mapping and Diversity in Plants and Animals
- Soybean genetics and cultivation
- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Genetic factors in colorectal cancer
- Genetics and Plant Breeding
- DNA Repair Mechanisms
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Nematode management and characterization studies
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
- Genetic and Environmental Crop Studies
- Plant Virus Research Studies
- Plant pathogens and resistance mechanisms
- Plant Pathogens and Resistance
- Lipid metabolism and biosynthesis
- Potato Plant Research
- Bryophyte Studies and Records
- Plant Micronutrient Interactions and Effects
- Genomics and Chromatin Dynamics
- Yeasts and Rust Fungi Studies
- Peatlands and Wetlands Ecology
Université Laval
2009-2023
McGill University
1996-1998
Université de Montréal
1991-1992
Highly parallel SNP genotyping platforms have been developed for some important crop species, but these typically carry a high cost per sample first-time or small-scale users. In contrast, recently by sequencing (GBS) approaches offer highly effective alternative simultaneous discovery and genotyping. the present investigation, we explored use of GBS in soybean. addition to developing novel analysis pipeline call SNPs indels from resulting sequence reads, devised modified library preparation...
An improved genetic linkage map has been constructed for cowpea (Vigna unguiculata L. Walp.) based on the segregation of various molecular markers and biological resistance traits in a population 94 recombinant inbred lines (RILs) derived from cross between 'IT84S-2049' '524B'. A set 242 markers, mostly amplified fragment length polymorphism (AFLP), linked to 17 traits, genes, gene analogs (RGAs) were scored within parental lines. These data used conjunction with 181 random polymorphic DNA...
Summary We examined the effects of substrate divergence and DNA mismatch repair (MMR) on recombination in Arabidopsis thaliana . Relative to frequency observed plants with a homologous construct (0% divergence), was decreased 4.1‐, 9.6‐, 11.7‐ or 20.3‐fold, respectively, lines constructs containing 0.5%, 2%, 4% 9% between substrates. To evaluate contribution MMR system this decrease, 12 independent reporter (two three per construct) were crossed an AtMSH2 T‐DNA insertional mutant. progeny...
Estimation of allelic frequencies is often required in breeding but genotyping many individuals at loci can be expensive. We have developed a genotyping-by-sequencing (GBS) approach for estimating on pooled samples (Pool-GBS) and used it to examine segregation distortion doubled haploid (DH) populations barley ( L.). In the first phase, we genotyped each line individually exploited these data explore strategy call single nucleotide polymorphisms (SNPs) reads. measured both number SNPs called...
The oilseed rape plant, Brassica napus, possesses two endogenous male sterile cytoplasms, nap and pol. Previous studies have shown that nuclear restoration of pol cytoplasmic sterility (CMS) is conditioned by a gene, Rfp, also involved in modifying transcripts the CMS-associated orf224/atp6 mtDNA region. We now find restorer gene Rfn apparently identical to Mmt, conditions modification from several different regions, including one associated with CMS contains orf222, chimeric related orf224....
Abstract Previous studies have shown that the mitochondrial orf224/atp6 gene region is correlated with Polima (pol) cytoplasmic male sterility (CMS) of Brassica nupus. We now extend this correlation by showing effects nuclear fertility restoration on transcripts cosegregate pol restorer Rfp1 in genetic crosses. also show, however, recessive rpf1 allele, or a very tightly linked gene, acts as dominant designated Mmt (modifier transcripts), controlling presence additional smaller nad4 and...
Summary The eukaryotic DNA mismatch repair (MMR) system contributes to maintaining genome integrity and sequence fidelity in at least two important ways: by correcting errors arising during replication, also preventing recombination events between divergent sequences. This study aimed investigate the role of one key MMR gene recombination. We obtained a mutant line which AtMLH1 has been disrupted insertion T‐DNA within coding region. Transcript analysis indicated that no full‐length...
Cyst nematodes are important agricultural pests responsible for billions of dollars losses each year. Plant resistance is the most effective management tool, but it requires a close monitoring population genetics. Current technologies pathotyping and genotyping cyst time-consuming, expensive imprecise. In this study, we capitalized on reproduction mode to develop simple genetic analysis pipeline based genotyping-by-sequencing Pool-Seq. This method yielded thousands SNPs allowed us study...
Genetic improvement of soybean, one the major crops providing edible oil and protein-rich food, is important to ensure balanced nutrition for growing world population. To make soybean cultivation more rewarding, an increase in seed protein content most desirable. Here, a critical review efforts employed over half-century accomplish has been presented. Many studies have used diverse parental lines map characterize quantitative trait loci (QTL)/genes regulating these two essential traits. we...
Abstract The soybean cyst nematode (SCN) ( Heterodera glycines Ichinohe) generates more damage to [ Glycine max (L.) Merr.] than any other parasite in most soybean‐producing countries. use of SCN‐resistant cultivars remains the effective method limit losses caused by SCN. accession PI 88788 has been used almost exclusively control SCN over past decades, inducing a shift virulence overcome resistance. Furthermore, and sources resistance characterized date belong maturity groups (MGs) III...
Summary A reporter system using engineered introns as recombination substrates in the uidA ( GUS ) gene has been developed and characterized Arabidopsis thaliana . The non‐coding nature of substrate allowed us to monitor events between duplicated copies intron that are either identical (homologous recombination) or harbour sequence polymorphisms (homoeologous recombination). effects length divergence on frequency were examined. positive correlation somatic was found increased 183‐fold when...
Genotyping-by-sequencing (GBS) is a relatively low-cost high throughput genotyping technology based on next generation sequencing and applicable to orphan species with no reference genome. A combination of genome complexity reduction multiplexing DNA barcoding provides simple affordable way resolve allelic variation between plant samples or populations. GBS was performed ApeKI libraries using from 48 genotypes each two heterogeneous populations tetraploid alfalfa (Medicago sativa spp....
Soybean (Glycine max (L.) Merr.) is the most important legume in world. However, rapid expansion of its cultivated areas has created new ecological niches for many pathogens. Among them, Phytophthora sojae (Kaufmann and Gerdemann) ranks as one damaging soybean pests The common method to control it introgression resistance genes termed Rps (Resistance P. sojae) into elite cultivars. This imposes a high selection pressure on leading development virulent pathotypes. Consequently, more durable...
Abstract Grain size is a key agronomic trait that contributes to grain yield in hexaploid wheat. length and width were evaluated an international collection of 157 wheat accessions. These accessions genetically characterized using genotyping-by-sequencing (GBS) protocol produced 73,784 single nucleotide polymorphism (SNP) markers. GBS-derived genotype calls obtained on Chinese Spring proved extremely accurate when compared the reference (> 99.9%) showed > 95% agreement with made at SNP...
Microsatellites are simple, tandem DNA repeats that represent unstable regions of the genome. They undergo frequent changes in tract length by base additions or deletions due to polymerase slippage during replication. To characterize factors affecting frequency spontaneous mutations occurring microsatellites plants, a reporter system was used Arabidopsis thaliana and tomato ( Lycopersicon esculentum ). The β-glucuronidase (GUS) measure mutation various (G 7 , G 10 13 16 C ) somatic tissues....
Abstract Maximizing yield is very important when developing new cultivars. However, must usually be improved jointly with other key traits, which can prove challenging they are unfavorably correlated. Genomic predictions facilitate the selection of promising lines among progeny crosses, but it may also help in selecting crosses that more likely to produce by predicting performance for various traits considered jointly. To assess whether genomic cross could breeders simultaneously improve...
Although biological N fixation (BNF) is a key process for economic cultivation of soybean [ Glycine max (L.) Merr.], the trait often neglected in breeding programs, mainly due to difficulties evaluating nodulation and parameters. We used genotyping‐by‐sequencing approach provide dense genome‐wide marker coverage with 1448 single nucleotide polymorphisms (SNPs) distributed broadly across chromosomes population. The mapping population was composted 113 F 7:8 recombinant inbred lines, obtained...
Habitat connectivity is important in conservation since isolation can diminish the potential of a population for adaptation and increase its risk extinction. However, naturally patchy ecosystems such as peatlands has mainly focused on preserving specific sites with exceptional characteristics, neglecting interconnectivity between patches. In order to better understand plant dynamics within peatland network, we assessed effect genetic distinctiveness, phenotypic variations germination rates...