Highly parallel SNP genotyping platforms have been developed for some important crop species, but these typically carry a high cost per sample first-time or small-scale users. In contrast, recently by sequencing (GBS) approaches offer highly effective alternative simultaneous discovery and genotyping. the present investigation, we explored use of GBS in soybean. addition to developing novel analysis pipeline call SNPs indels from resulting sequence reads, devised modified library preparation...

Soya bean is a major source of edible oil and protein for human consumption as well animal feed. Understanding the genetic basis different traits in soya will provide important insights improving breeding strategies this crop. A genome-wide association study (GWAS) was conducted to accelerate molecular improvement agronomic bean. genotyping-by-sequencing (GBS) approach used dense marker coverage (>47,000 SNPs) panel 304 short-season lines. subset 139 lines, representative diversity among...

The role and essentiality of silicon (Si) in plant biology have been debated for >150 years despite numerous reports describing its beneficial properties. To obtain unique insights regarding the effect Si on plants, we performed a complete transcriptome analysis both control powdery mildew-stressed Arabidopsis with or without application, using 44K microarray. Surprisingly, expression all but two genes was unaffected by result contradicting possible direct as fertilizer. In contrast,...

Summary The controversy surrounding silicon (Si) benefits and essentiality in plants is exacerbated by the differential ability of species to absorb this element. This property seemingly enhanced carrying specific nodulin 26‐like intrinsic proteins ( NIP s), a subclass aquaporins. In work, our aim was characterize plant aquaporins define features that confer Si permeability. Through comparative analysis 985 25 with differing abilities Si, we were able predict 30 transporters discovered...

Background: Cerebral intra-arterial chemotherapy (CIAC) has been demonstrated to achieve tumoricidal concentrations in cerebral tumour cells that are otherwise unachievable due the presence of blood–brain barrier. In this study, we sought analyze safety CIAC a cohort patients treated at Centre intégré universitaire de santé et services sociaux l’Estrie—Centre hospitalier Sherbrooke (CIUSSS-CHUS). Methods: Treatments consisted monthly CIAC. A neurological examination and neuroimaging study...

In yeast, the DMC1 gene is required for interhomolog recombination, which an essential step bivalent formation and correct partition of chromosomes during meiosis I. By using a reverse genetics approach, we were able to identify T-DNA insertion in AtDMC1, Arabidopsis homolog DMC1. Homozygotes AtDMC1 failed express their residual fertility was 1.5% that wild type. Complete restored mutant plants when wild-type copy reintroduced. Cytogenetical analysis points correlation sterility phenotype...

Summary Roads provide suitable conditions for the establishment and growth of exotic species. Most roads are bordered by drainage ditches forming a network linear wetlands. Drainage may serve as habitats corridors facilitating spread aquatic invaders into intersected ecosystems. The common reed Phragmites australis is one these frequently found in marshes along roads. We hypothesized that highways have acted dispersal contributed to invasion North American wetlands this mapped spatial...

Sclerotinia stem rot (SSR) is one of the most important pests in cool soybean growing regions Northeastern United States and Canada. However, intensity infestations varies considerably from year to according weather conditions, thus making it difficult for breeders select under uniform disease pressure. Selection resistance SSR would be greatly facilitated by use molecular markers. In this work, a collection 130 lines was inoculated using cotton pad method genetically characterized...

The supply of soluble silicon (Si) to plants has been associated with many benefits that remain poorly explained and often contested. In this work, the effect Si was studied on wheat under both control pathogen stress (Blumeria graminis f. sp. tritici) conditions by conducting a large transcriptomic analysis (55,000 unigenes) aimed at comparing differential response four treatments. (uninfected) limited 47 genes diverse functions providing little evidence regulation specific metabolic...

Next-generation sequencing (NGS) has revolutionized plant and animal research in many ways including new methods of high throughput genotyping. Genotyping-by-sequencing (GBS) been demonstrated to be a robust cost-effective genotyping method capable producing thousands millions SNPs across wide range species. Undoubtedly, the greatest barrier its broader use is challenge data analysis. Herein we describe comprehensive comparison seven GBS bioinformatics pipelines developed process raw...

Next-generation sequencing (NGS) technologies have accelerated considerably the investigation into composition of genomes and their functions. Genotyping-by-sequencing (GBS) is a genotyping approach that makes use NGS to rapidly economically scan genome. It has been shown allow simultaneous discovery thousands millions SNPs across wide range species. For most users, main challenge in GBS bioinformatics analysis large amount sequence information derived from libraries view calling alleles at...

Sclerotinia stem rot (SSR) is the most important soybean disease in Eastern Canada. The development of resistant cultivars represents cost-effective means limiting impact this disease. In view ensuring durable resistance, it imperative to identify germplasm harbouring different resistance loci and provide breeders with closely linked molecular markers facilitate breeding. With end view, we assessed using a highly reproducible artificial inoculation method on diverse collection 101 lines,...

Plants benefit greatly from silicon (Si) absorption provided that they contain Si transporters. The latter have recently been identified in the roots of some higher plants known to accumulate high concentrations Si, and all share a level sequence identity. In this study, we searched for transporters primitive vascular plant Equisetum arvense (horsetail), which is valuable but neglected model study absorption, as it has one highest kingdom. Our initial attempts identify based on homology with...

Silicon (Si) is known to protect against biotrophic and hemibiotrophic plant pathogens; however, the mechanisms by which it exerts its prophylactic role remain unknown. In an attempt obtain unique insights into mode of action Si, we conducted a full comparative transcriptomic analysis soybean (Glycine max) plants Phytophthora sojae, hemibiotroph that relies heavily on effectors for virulence. Supplying Si inoculated provided strong protection P. sojae over course experiment (21 day). Our...

Studies on structural variation in plants have revealed the inadequacy of a single reference genome for an entire species and suggest that it is necessary to build species-representative called pan-genome better capture extent both nucleotide variation. Here, we present cultivated soybean (Glycine max), termed PanSoy, constructed using de novo assembly 204 phylogenetically geographically representative improved accessions selected from larger GmHapMap collection. PanSoy uncovers 108 Mb...

ABSTRACT Using 779 herbarium and modern specimens, we reconstructed the spread of common reed ( Phragmites australis ) in Quebec, Canada, where large‐scale invasion this plant species has been reported since 1960s. All specimens were genetically differentiated using molecular tools to identify genotype (native or exotic). The exotic (haplotype M) present Quebec as early 1916, but it was rare prior 1970s almost exclusively restricted shores St. Lawrence River. inland only after beginning...

Abstract In crop breeding, the interest of predicting performance candidate cultivars in field has increased due to recent advances molecular breeding technologies. However, complexity wheat genome presents some challenges for applying new technologies marker identification with next-generation sequencing. We applied genotyping-by-sequencing, a recently developed method identify single-nucleotide polymorphisms, genomes 384 (Triticum aestivum) genotypes that were tested under three different...

Here, we describe a worldwide haplotype map for soybean (GmHapMap) constructed using whole-genome sequence data 1007 Glycine max accessions and yielding 14.9 million variants as well 4.3 M tag single-nucleotide polymorphisms (SNPs). When sampling random subsets of these accessions, the number SNPs plateaued beyond approximately 800 600 respectively. This suggests extensive coverage diversity within cultivated soybean. GmHapMap were imputed onto 21 618 previously genotyped with up to 96%...