A5045856861- Soybean genetics and cultivation
- Yeasts and Rust Fungi Studies
- Plant Disease Resistance and Genetics
- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Resistance
- Plant pathogens and resistance mechanisms
- Soil Management and Crop Yield
- Plant Pathogens and Fungal Diseases
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Banana Cultivation and Research
- Cassava research and cyanide
- Chromosomal and Genetic Variations
- Growth and nutrition in plants
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Molecular Biology Techniques and Applications
- Plant Disease Management Techniques
- Plant tissue culture and regeneration
- Phytase and its Applications
- Peanut Plant Research Studies
- Rural Development and Agriculture
- Seed Germination and Physiology
- Agricultural and Food Sciences
Brazilian Agricultural Research Corporation
2016-2025
Universidade Estadual de Londrina
2016-2020
Université Laval
2019
Universidade Federal de Pernambuco
2012
United States Department of Agriculture
2009
Agricultural Research Service
2009
Iowa State University
2009
University of Nebraska–Lincoln
2003-2007
Universidade Federal de Viçosa
1995
Abstract Background Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in eukaryotes. Current studies have demonstrated microRNAs (miRNAs) act several plant pathways associated with tissue proliferation, differentiation, development response to abiotic biotic stresses. In order identify new miRNAs soybean verify those possibly water deficit rust-stress regulated, eight libraries small were constructed...
The plant mitochondrial genome is retained in a multipartite structure that arises by process of repeat-mediated homologous recombination. Low-frequency ectopic recombination also occurs, often producing sequence chimeras, aberrant ORFs, and novel subgenomic DNA molecules. This genomic plasticity may distinguish the mitochondrion from mammalian fungal types. In plants, relative copy number recombination-derived molecules within mitochondria controlled nuclear genes, shifting can result their...
Abstract Background The Hsp 20 genes are associated with stress caused by HS and other abiotic factors, but have recently been found to be the response biotic stresses. These represent most abundant class among HSPs in plants, little is known about this gene family soybean. Because of their apparent multifunctionality, these proteins promising targets for developing crop varieties that better adapted Thus, present study an silico identification Gm members was performed, were characterized...
Asian soybean rust (ASR), caused by Phakopsora pachyrhizi, is now established in all major soybean-producing countries. Currently, there little information about the molecular basis of ASR-soybean interactions, which will be needed to assist future efforts develop effective resistance. Toward this end, abundance changes mRNAs were measured over a 7-day ASR infection time course mock-inoculated and infected leaves accession (PI230970) carrying Rpp2 resistance gene susceptible genotype...
Stability of the mitochondrial genome is controlled by nuclear loci. In plants, genes suppress DNA rearrangements during development. One gene involved in this process Msh1. Msh1 appears to be suppression illegitimate recombination plant mitochondria. To test hypothesis that disruption leads type associated with naturally occurring cytoplasmic male sterility a transgenic approach for RNAi was used modulate expression tobacco and tomato. both species, these experiments resulted reproducible...
Asian soybean rust is a formidable threat to (Glycine max) production in many areas of the world, including United States. Only five sources resistance have been identified (Resistance Phakopsora pachyrhizi1 [Rpp1], Rpp2, Rpp3, Rpp4, and Rpp5). Rpp4 was previously resistant genotype PI459025B mapped within 2 centimorgans Satt288 on chromosome 18 (linkage group G). Using simple sequence repeat markers, we developed bacterial artificial contig for locus susceptible cv Williams82 (Wm82)....
Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is causal agent of Asian Soybean Rust, one most important diseases. There evidences WRKYs resistance some genotypes against fungus. The number genes already annotated genome was underrepresented. In present study, a genome-wide annotation family carried out members P. were identified. As result genomic databases search, 182...
Here, we describe a worldwide haplotype map for soybean (GmHapMap) constructed using whole-genome sequence data 1007 Glycine max accessions and yielding 14.9 million variants as well 4.3 M tag single-nucleotide polymorphisms (SNPs). When sampling random subsets of these accessions, the number SNPs plateaued beyond approximately 800 600 respectively. This suggests extensive coverage diversity within cultivated soybean. GmHapMap were imputed onto 21 618 previously genotyped with up to 96%...
Soybean [Glycine max (L.) Merrill] is one of the most important legumes cultivated worldwide, and Brazil main producers this crop. Since sequencing its reference genome, interest in structural allelic variations wild soybean germplasm has grown. To investigate genetics Brazilian germplasm, we selected cultivars based on year commercialization, geographical region maturity group resequenced their genomes. We genomes 28 with an average genome coverage 14.8X. A total 5,835,185 single nucleotide...
In recent years soybean rust, caused by Phakopsora pachyrhizi has become one of the most serious threats to production in Brazil. Breeding lines and varieties have been selected for resistance rust Asia. However, differences virulence between Asian Brazilian populations should be considered order select use resistant resources from Here, we suggest criteria distinguishing susceptible types analysis four characters: frequency lesions having uredinia, number uredinia per lesion, open...
Rpp4 (Resistance to Phakopsora pachyrhizi 4) confers resistance Sydow, the causal agent of Asian soybean rust (ASR). By combining expression profiling and virus induced gene silencing (VIGS), we are developing a genetic framework for Rpp4-mediated resistance. We measured in mock-inoculated P. pachyrhizi-infected leaves resistant accession PI459025B (Rpp4) susceptible cultivar (Williams 82) across 12-day time course. Unexpectedly, two biphasic responses were identified. In incompatible...
Soybean genotypes MG/BR46 (Conquista) and BR16, drought-tolerant -sensitive, respectively, were compared in terms of morphophysiological gene-expression responses to water stress during two stages development.Gene-expression analysis showed differential Gmdreb1a Gmpip1b mRNA expression within 30 days water-deficit initiation plants.Within 45 initiating stress, Gmp5cs had relatively higher expression.Initially, BR16 increased only for Gmdreb1a, later (45 days) Gmp5cs, Gmdefensin Gmpip1b.Only...
In this study, the influence of genetic background on resistance level a soybean line carrying Rpp2, Rpp4, and Rpp5 was evaluated by backcrossing it with susceptible variety. It also eight lines which carry these Rpp genes against five Asian rust (ASR) isolates, in order to determine likely range ASR isolates differing pathogenicity. The results indicated that high various could be retained three backgrounds, although minor influences plant pathogenicity occur. Thus, pyramided should...
We evaluated the molecular, anatomical and physiological properties of a soybean line transformed to improve drought tolerance with an rd29A:AtDREB1A construct.This construct expressed dehydrationresponsive element binding protein DREB1A from stress-inducible rd29A promoter.The greenhouse growth test included four randomized blocks plants, each treatment performed in triplicate.Seeds non-transformed cultivar BR16 genetically modified P58 (T 2 generation) were grown at 15% gravimetric...
Soybean [ Glycine max (L.) Merrill] is one of the most widely grown legumes in world, with Brazil being its largest producer and exporter. Breeding programs have resulted from multiple cycles selection recombination starting a small number USA cultivar ancestors 1950s 1960s years. This process has led to successful adaptation this crop tropical conditions, phenomenon known as tropicalization. Many studies describe narrow genetic background Brazilian soybean cultivars. Various factors can...
Macrophomina phaseolina has been considered one of the most prevalent soybean (Glycine max) pathogens in Brazil. No genetic resistance determined and very little is known about diversity this pathogen tropical sub-tropical regions. Fifty-five isolates from roots were collected different regions analyzed through RAPD for diversity. The UPGMA cluster analysis 74 loci scored permitted identification three divergent groups with an average similarity 99%, 92% 88%, respectively. corresponded to...
Asian soybean rust (ASR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, can cause losses greater than 80%. Despite its economic importance, there is no cultivar with durable ASR resistance. In addition, P. pachyrhizi genome not yet available. However, availability of other genomes, as well development sample enrichment strategies and bioinformatics tools, has improved our knowledge secretome potential effectors. this context, we used a combination laser capture...