Abstract Purpose Glycogen storage disease type III (GSD III) is a rare inherited metabolic characterized by excessive accumulation of glycogen in liver, skeletal muscle, and heart. Currently, there are no widely available noninvasive methods to assess tissue levels load. Here, we use nuclear Overhauser effect (glycoNOE) MRI quantify hepatic mouse model GSD III. Methods Agl knockout mice ( n = 13) wild‐type controls 10) were scanned for liver content using glycoNOE MRI. All fasted 12 16 h...

Chemical exchange saturation transfer (CEST) magnetic resonance imaging has shown promise for classifying tumors based on their aggressiveness, but CEST contrast is complicated by multiple signal sources and thus prolonged acquisition times are often required to extract the of interest. We investigated whether deep learning could help identify pertinent Z‐spectral features distinguishing tumor aggressiveness as well possibility acquiring only spectral regions more efficient acquisition....

Abstract Chemical exchange saturation transfer (CEST) MRI at 3 T suffers from low specificity due to overlapping CEST effects multiple metabolites, while higher field strengths ( B 0 ) allow for better separation of Z ‐spectral “peaks,” aiding signal interpretation and quantification. However, data acquisition is restricted by equipment access, inhomogeneity safety issues. Herein, we aim synthesize higher‐ ‐spectra readily available acquired with clinical scanners using a deep learning...

Background: Chemical exchange saturation transfer (CEST) MRI requires the acquisition of multiple saturation-weighted images and can last several minutes. Misalignments among these images, which are often due to inevitable motion subject, will corrupt CEST contrast maps result in large quantification errors. Therefore, registration series is critical. However, challenging since common intensity-based algorithms may fail differentiate signals from artifacts. Herein, we studied how different...

Background: Chemical exchange saturation transfer (CEST) MRI is a promising approach for detecting biochemical alterations in cancers and neurological diseases, but the quantification can be challenging. Among numerous methods, Lorentzian difference (LD) relatively simple widely used, which employs line-shape as reference to describe direct (DS) of water takes account against experimental CEST spectra data. However, LD often overestimates nuclear overhauser enhancement (NOE) effects....

Mannitol is a hyperosmolar agent for reducing intracranial pressure and inducing osmotic blood-brain barrier opening (OBBBO). There great clinical need non-invasive method to optimize the safety of mannitol dosing. The aim this study was develop label-free Chemical Exchange Saturation Transfer (CEST)-based MRI approach detecting accumulation following OBBBO.In vitro conducted measure CEST properties D-mannitol different concentrations pH. In vivo MRS measurements were on Sprague-Dawley rats...

Background: Quantification of in vivo chemical exchange saturation transfer (CEST) magnetic resonance signals is challenging due to contamination from coexisting effects, including the direct water effect and asymmetric magnetization transfer. Fitting-based analysis allows calculation multiple types line shape Z-spectra. However, conventional voxelwise method has several drawbacks, its long computation time susceptibility image noise Z-spectra oscillations, it difficult determine initial...

Purpose Saturation transfer MRI has previously been used to probe molecular binding interactions with signal enhancement via the water signal. Here, we detail relayed nuclear overhauser effect (rNOE) based mechanisms of this enhancement, develop a strategy quantifying affinity, i.e., dissociation constant (), and apply method detect electrostatic several charged small biomolecules. Another goal was estimate detection limit for transient receptor‐substrate binding. Theory Methods The...

Purpose Acquisition of high‐resolution Z‐spectra for CEST or magnetization transfer contrast (MTC) MRI requires excessive scan times. Ultrafast Z‐spectroscopy (UFZ) has been proposed to address this; however, the quality in vivo UFZ spectra insufficient. Here, we present a simple approach improve this. Theory and Methods imaging acquires full by encoding spectral dimension spatially via gradient applied concurrently with RF saturation pulse. Different from previous implementations, both this...

Glycogen storage disease type III (GSD III) is characterized by abnormally high glycogen accumulation in liver, muscle, and heart. Treatments such as diet modification drugs are currently being developed, but there a lack of suitable methods for assessing load possible treatment efficacy. Recently, it was shown that glycoNOE MRI can image levels vivo here we apply this technique to distinguish GSD from controls mouse model. The results show clearly contrast exhibits linear correlation with...

Acquisition of high-resolution Z-spectra requires excessive scan times. Ultrafast Z-spectroscopy (UFZ) can obtain whole within one acquisition by encoding the Z-spectral dimension spatially via a gradient applied concurrently with saturation RF pulse, significantly reducing scanning time. Still, UFZ has had limited success in vivo due to tissue heterogeneity. Here, we developed TS-UFZ imaging approach where both and its readout were slice direction, there is minimal Results show that allows...

Motivation: The metabolic heterogeneities in human are high, it is crucial to improve the slice-encoding coverage phosphocreatine and glycogen mapping. Goal(s): To develop a 3D-CEST sequence for simultaneous mapping of within acceptable time. Approach: optimal using stack-of-star readouts was applied. patch-based low-rank reconstruction introduced accelerate scan. concentrations were quantified with ex-vivo in-vivo experiments. Results: dimension improved 140 mm. scan time reduced from 41.8...

Pompe disease (PD) is a glycogen storage disease, characterized by progressive lysosomal accumulation and changes in energy metabolite levels. There lack of methods to noninvasively assess PD progression treatment response. We demonstrate that saturation transfer MRI can detect glycogen, total creatine (tCr), phosphocreatine (PCr) mouse model show more than doubling muscle levels gradual decrease tCr PCr with age. The simultaneous mapping these metabolites has potential other diseases...

Glycogen and phosphocreatine (PCr) are essential metabolites for maintaining cellular ATP levels. Recent reports show the possibility of PCr mapping in skeletal muscle using CEST MRI but glycogen levels measured this approach have been inconclusive. Here we report high-resolution human based on relayed nuclear Overhauser effects (glycoNOE) CEST, respectively. Results at 5T homogeneous distributions calf corresponding to biopsy. Exciting possible applications include localized studies...

There is a need for non-invasive approaches to assess the progression of glycogen storage diseases (GSD). Here, we use saturation transfer (ST) MRI via relayed nuclear Overhauser effects (glycoNOE) detect abnormal changes in muscle GSD II mouse model. In addition glycogen, energy metabolites phosphocreatine (PCr) and creatine (Cr) were studied disease. Water (Z-spectra) 1H MRS acquired at 9.4 T on skeletal healthy control mice homozygous acid α$$ \upalpha $$ -glucosidase (GAA) knock-out...

Motivation: High-resolution brain thermometry remains challenging. Goal(s): To estimate the feasibility of creatine chemical exchange saturation transfer (CrCEST) imaging for thermometry. Approach: The CrCEST phantom and swine was conducted at various temperatures on a 5.0T MR scanner (UIH Jupiter). relationship between apparent offset temperature estimated with regression analysis, based which maps were generated. Results: Strong linear relationships identified both (0.005ppm/oC) ex vivo...

Motivation: PCrCEST and CrCEST contrasts are weak indiscernible in clinical settings (≤ 3 T), limiting the accuracy of quantification. Goal(s): To develop an accurate reliable method human skeletal muscle at 5 T. Approach: The optimal saturation scheme was investigated. Two-peak PLOF employed for Plantar flexion exercise conducted to validate performance proposed method. Results: Distinct peaks can be observed simultaneously 5T. transition between PCr Cr clearly a volunteer during...

Brain temperature signifies the thermal homeostasis of tissue, and may serve as a marker for neuroprotective therapy. Currently, it remains challenging to map human brain with high spatial resolution. The dependence chemical exchange saturation transfer (CEST) effects endogenous labile protons provide promising mechanism absolute imaging. In this study, we aimed investigate dependency CEST effect creatine (CrCEST), contemplate its feasibility mapping. Creatine (Cr) was selected agent probe...

Probing regional glycogen metabolism in humans non-invasively has been challenging due to a lack of sensitive approaches. Here we studied human muscle dynamics post-exercise with spatial resolution millimeters and temporal minutes, using relayed nuclear Overhauser effect (glycoNOE) MRI. Data at 5T showed homogeneous distribution resting muscle, an average concentration 99 ± 13 mM. After plantar flexion exercise following fasting recovery under conditions, the calf spatially heterogeneous...

A robust image registration technique for motion correction of chemical exchange saturation transfer (CEST) MRI series was introduced in this paper. The method based on adaptive stochastic gradient descent optimization (ASGD), and tested the motion-corrupted Z-spectral vivo data a tumor-bearing mouse brain acquired an 11.7 Tesla scanner. Compared with conventional (GD) registration, both qualitative quantitative results indicated that ASGD allowed more accurate corrupted series.