A5061378680- Lipid Membrane Structure and Behavior
- Spectroscopy and Quantum Chemical Studies
- Sphingolipid Metabolism and Signaling
- Supramolecular Self-Assembly in Materials
- Protein Interaction Studies and Fluorescence Analysis
- Surfactants and Colloidal Systems
- Protein Structure and Dynamics
- Molecular Sensors and Ion Detection
- Cholesterol and Lipid Metabolism
- Receptor Mechanisms and Signaling
- Analytical Chemistry and Sensors
- Analytical Chemistry and Chromatography
- RNA Interference and Gene Delivery
- Caveolin-1 and cellular processes
- RNA and protein synthesis mechanisms
- Photoreceptor and optogenetics research
- Proteins in Food Systems
- Advanced biosensing and bioanalysis techniques
- Neuropeptides and Animal Physiology
- Nanopore and Nanochannel Transport Studies
- Lipid metabolism and biosynthesis
- Ion Transport and Channel Regulation
- Advanced Sensor and Energy Harvesting Materials
- Cellular transport and secretion
- Spectroscopy Techniques in Biomedical and Chemical Research
Temple University
2014-2024
Icahn School of Medicine at Mount Sinai
2017
Philadelphia University
2009
Mount Sinai Hospital
2007
Mount Sinai Medical Center
1998-2007
Meharry Medical College
1990-1993
University of Virginia
1985-1989
University of California, San Diego
1985
University of Illinois Urbana-Champaign
1984-1985
The University of Texas Health Science Center at Houston
1985
Recent attempts at rebuilding the myocardium using stem cells have yielded disappointing results. The lack of a supporting vasculature may, in part, explain these findings. However, concerns over possible side effects hampered revascularizing infarcted systemic delivery proangiogenic compounds. In this study, we develop technology to enhance morphology and function postinfarct neovasculature. Previously, shown that up-regulated expression endothelial cell adhesion molecules myocardial...
To investigate the mechanisms by which hydrostatic pressure inhibits (Na,K)-ATPase, we measured enzyme activity, as a function of and temperature, purified (Na,K)-ATPase from dog kidney eel electroplax, monitored protein conformation, possible subunit interactions, fluidity membrane with fluorescent probes.The p-nitrophenylphosphatase activities were inhibited reversibly pressures below 1.5 kilobars (eel enzyme) 2.5 (dog enzyme).Above these pressures, enzymes inactivated irreversibly.The...
To investigate the lateral organization of sterols in membranes, fluorescence intensity dehydroergosterol at different mole fractions liquid crystalline dimyristoyl phosphatidylcholine bilayers was examined. A number drops were observed specific fractions, as predicted from a hexagonal super-lattice model. The dips provide compelling evidence that naturally occurring sterol is regularly distributed fixed compositional consistent with presence super-lattices fluid membranes. Regularly...
The endosomal-sorting complex required for transport (ESCRT) is evolutionarily conserved from Archaea to eukaryotes. drives membrane scission events in a range of processes, including cytokinesis Metazoa and some Archaea. CdvA the protein that recruits ESCRT-III membrane. Using electron cryotomography (ECT), we find polymerizes into helical filaments wrapped around liposomes. proteins are responsible cinching membranes have been shown assemble tubes vitro, but here show they also can form...
The physical origin of the extremely high thermal stability tetraether liposomes composed polar lipid fraction E (PLFE) from thermoacidophilic archaebacterium Sulfolobus acidocaldarius has been investigated. leakage rate trapped 5,6-carboxyfluorescein (5(6)CF) and proton permeability in PLFE have measured using fluorescence probe techniques temperature range 25−85 °C. results are compared with those obtained nonarchaebacterial liposomes. Egg yolk phosphatidylglycerol (eggPG) exhibit similar...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPressure dependence of 1,6-diphenyl-1,3,5-hexatriene fluorescence in single-component phosphatidylcholine liposomesParkson L. G. Chong and Gregorio WeberCite this: Biochemistry 1983, 22, 24, 5544–5550Publication Date (Print):November 1, 1983Publication History Published online1 May 2002Published inissue 1 November 1983https://pubs.acs.org/doi/10.1021/bi00293a014https://doi.org/10.1021/bi00293a014research-articleACS PublicationsRequest reuse...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA differential polarized phase fluorometric study of the effects high hydrostatic pressure upon fluidity cellular membranesParkson L. G. Chong, Andrew R. Cossins, and Gregorio WeberCite this: Biochemistry 1983, 22, 2, 409–415Publication Date (Print):January 18, 1983Publication History Published online1 May 2002Published inissue 18 January 1983https://pubs.acs.org/doi/10.1021/bi00271a026https://doi.org/10.1021/bi00271a026research-articleACS...
Lipid rafts are microdomains of plasma membranes enriched in cholesterol and sphingolipids the outer layer. We determined whether κ opioid receptors (KOR) human placenta FLAG (DYKDDDDK)-tagged KOR (FLAG-hKOR) expressed Chinese hamster ovary (CHO) cells localized lipid changes contents affect hKOR properties signaling. were prepared from CHO expressing FLAG-hKOR using Na<sub>2</sub>CO<sub>3</sub> method fractionation through a sucrose density gradient. The majority cells, by...
Nystatin isolated from Streptomyces is a polyene antibiotic that frequently used in the treatment and prophylaxis of fungal infections. Here, fractional sterol concentration dependencies partition coefficient for partitioning nystatin into ergosterol/dimyristoyl-l-α-phosphatidylcholine (DMPC), cholesterol/DMPC, ergosterol/1-palmitoyl-2-oleoyl-l-α-phosphatidylcholine (POPC), ergosterol/POPC/1-palmitoyl-2-oleoyl-l-α-phosphatidylethanolamine (POPE) multilamellar vesicles have been determined...
Here, the interplay between membrane cholesterol lateral organization and activity of surface-acting enzymes was addressed using soil bacteria oxidase (COD) as a model. Specifically, effect mole fraction on initial rate oxidation catalyzed by COD investigated at 37 degrees C cholesterol/1-palmitoyl-2-oleoyl-l-alpha-phosphatidylcholine (POPC) large unilamellar vesicles (LUVs, approximately 800 nm in diameter). In three concentration ranges examined (18.8-21.2, 23.6-26.3, 32.2-34.5 mol %...