A5057131951- Chemical Synthesis and Analysis
- Peptidase Inhibition and Analysis
- Carbohydrate Chemistry and Synthesis
- RNA Interference and Gene Delivery
- Biochemical and Structural Characterization
- Enzyme Production and Characterization
- Receptor Mechanisms and Signaling
- Immunotherapy and Immune Responses
- Empathy and Medical Education
- Neuropeptides and Animal Physiology
- Perfectionism, Procrastination, Anxiety Studies
- Glycosylation and Glycoproteins Research
Technical University of Darmstadt
2024
University of Copenhagen
2023
Utrecht University
2017-2019
De novo macrocyclic peptides, derived using selection technologies such as phage and mRNA display, present unique unexpected solutions to challenging biological problems. This is due in part their unusual folds, which are able side chains ways not available canonical structures α-helices β-sheets. Despite much recent interest these molecules, folding binding behavior remains poorly characterized. In this work, we cocrystallization, docking, solution NMR of three de peptides that all bind...
Targeting the tumor microenvironment (TME) is an attractive strategy for cancer therapy, as cells in vivo are surrounded by many different influential cell types, with complex interactions strongly affecting progression and therapeutic outcome. Cancer-associated fibroblasts (CAFs) represent abundant stromal type TME that modulate development exerting immunosuppressive effect to influence effector immune activation. One promising target TME-directed therapy CAF marker fibroblast activation...
The 57-mer full-length GPR15L(25-81) peptide has been identified as the principal endogenous agonist of G protein-coupled receptor GPR15. Its main activity resides in C-terminal 11-mer GPR15L(71-81), which full efficacy but ~40-fold lower potency than peptide. Here, we systematically investigated structure-activity relationship GPR15L(71-81) by truncations/extensions, alanine-scanning, and N- capping. synthesized analogues were tested at GPR15 stably expressed HEK293A cells using a...
Abstract Retaining glycosidases are an important class of enzymes involved in glycan degradation. To study better the role specific deglycosylation processes, and thereby importance particular glycosylation patterns, a set potent inhibitors, each to glycosidase, would be invaluable toolkit. Towards this goal, we detail here more in‐depth prototypical macrocyclic peptide inhibitor model retaining glycosidase human pancreatic α‐amylase (HPA). Notably, incorporation l ‐DOPA into affords HPA...