A growing number of long non-coding RNAs (lncRNAs) have been linked to squamous cell carcinoma the head and neck (SCCHN). subclass lncRNAs, termed enhancer (eRNAs), are derived from regions could contribute function. In this study, we developed an integrated data analysis approach identify key eRNAs in SCCHN. Tissue-specific enhancer-derived their regulated genes previously predicted using computational pipeline PreSTIGE, were considered as putative eRNA-target pairs. The interactive web...

MicroRNAs (miRNAs) are small noncoding RNA molecules with an essential role in regulation of gene expression. miRNA expression profiles differ between tumor and normal control tissue many types cancers profiling is seen as a promising field for finding new diagnostic prognostic tools.In this study, we have analyzed three miRNAs, miR-21, miR-125b, miR-203, their potential target proteins p53 p63, known to be deregulated squamous cell carcinoma the head neck (SCCHN), two distinct one mixed...

Abstract The human p63 gene codes for multiple protein isoforms and is commonly over‐expressed in squamous cell carcinoma of head neck (SCCHN). This expression predominantly the ΔN‐ β‐isoforms, former lacking p53‐related transactivation domain. can activate or repress transcription p53 p73 target genes, but also has unique transcriptional targets and, unlike other family members, required normal development differentiation epithelia. We have identified novel p63, using microarray analysis...

Oral lichen planus (OLP) is a chronic inflammatory disease of unknown origin, showing little spontaneous regression. WHO classifies OLP as premalignant condition, however, the underlying mechanisms initiating development cancer in lesions are not understood. The p53 tumour suppressor plays an important role many tumours, and increased expression protein has been seen lesions. Recently it was shown that human TP53 gene encodes at least nine different isoforms. Another member family, p63,...

J Oral Pathol Med (2012) 41 : 90–95 Background: lichen planus (OLP), which is a chronic inflammatory disease of the oral mucosa with unknown etiology, affects about 2% population. MicroRNAs are small non‐coding RNAs involved in normal processes such as development and differentiation well progression human diseases. The aim this study was to investigate expression miR‐21, miR‐125b, miR‐203 compare RNA levels their potential targets, tumor suppressor p53 its relative p63, both known be...

Abstract Background Oral lichen planus (OLP) is a chronic inflammatory disease for which the pathogenesis not fully understood. OLP has autoimmune features and auto immunity been suggested as potential cause, whereas WHO classified premalignant condition. Association between inflammation cancer known one of characteristics OLP. A protein connected to be involved in development cyclooxygenase‐2 (COX‐2) can inhibited by microRNA‐26b (miR‐26b). Objective The aim was map levels COX‐2 miR‐26b...

The overall survival for patients with squamous cell carcinoma of the tongue is low and search early diagnostic prognostic markers thus essential. MicroRNAs have been suggested as potential candidates in head neck general.On basis known differences between sub-sites within oral cavity, we investigated expression role microRNA-424 arising tongue. MicroRNA levels were measured by qRT-PCR both tissue plasma samples.Levels upregulated carcinoma, but not tumours originating from gingiva or floor...

// Linda Boldrup 1 , Xiaolian Gu Philip J. Coates 2 Lena Norberg-Spaak 3 Robin Fahraeus 1, 2, 4 Göran Laurell 3, 5 Torben Wilms Karin Nylander Department of Medical Biosciences/Pathology, Umeå University, SE – 901 85 Umeå, Sweden RECAMO, Masaryk Memorial Cancer Institute, 656 53 Brno, Czech Republic Clinical Sciences/ENT Institut de Génétique Moléculaire, Université Paris 7, Hôpital St. Louis, 75010 Paris, France Surgical Sciences/ENT,...

Abstract Epigenetic modifications are essential regulators of biological processes. Decreased DNA methylation OAS2 (2′-5′-Oligoadenylate Synthetase 2), encoding an antiviral protein, has been seen in psoriasis. To provide further insight into the epigenetic regulation , we performed pyrosequencing to detect status at 11 promoter and first exon located CpG sites psoriasis (n = 12) two common subtypes squamous cell carcinoma (SCC) head neck: tongue tonsillar 11). Compared corresponding...

Immune cells and cytolytic activity within the tumor microenvironment are being intensively studied. Through transcriptome profiling, immune cell enumeration using xCell tool quantification according to granzyme A (GZMA) perforin (PRF1) mRNA levels, we investigated immunoreactivity in and/or tumor-free tongue tissue samples from 31 patients with squamous carcinoma of oral 14 healthy individuals (control tissues). We found significantly altered compositions (p < 0.001) elevated compared...

J Oral Pathol Med (2012) 41 : 86–89 Background: Mucosal lichen planus is a severe variant of planus, Lichen (LP), which in many ways affect patients’ lives. The aetiology not fully understood, and there no treatment clearing the disease once for all. LP has by WHO been classified as precancerous lesion. Micro‐RNAs, miRNAs, are non‐coding, small single‐stranded RNAs involved biological processes like apoptosis, proliferation, differentiation, metastasis, angiogenesis immune response. Methods...

Background The incidence of squamous cell carcinoma the oral tongue ( SCCOT ) is increasing in people under age 40. There an urgent need to identify prognostic markers that help young patients with poor prognosis order select these for individualized treatment. Materials and methods To genetic can serve as patients, we first investigated four (≤40 years) five elderly (≥50 using global RNA sequencing whole‐exome sequencing. Next, combined our data on from cancer genome atlas TCGA ), giving a...

Transfer-RNA-derived fragments (tRFs) are a class of small non-coding RNAs that functionally different from their parental transfer (tRNAs). tRFs can regulate gene expression by several mechanisms, and involved in variety pathological processes. Here, we aimed at understanding the composition abundance squamous cell carcinoma head neck (SCCHN), evaluated potential as prognostic markers this cancer type. We obtained tRF data The Cancer Genome Atlas (TCGA) HNSC cohort (523 patients) using...

The p53 homologue p63 produces six different isoforms that are important in development of epithelial tissues and squamous cell carcinoma the head neck (SCCHN). In SCCHN, expression is highly complex, with over-expression DeltaNp63 p63beta many tumours. To date, little known about functions elucidating distinctive properties will help to clarify how they influence tumour biology. By gene profiling SCCHN cells over-expressing we identified effects three isoforms, DeltaNp63beta being more...

The pathogenesis of oral lichen planus (OLP), a chronic inflammatory disease, is not fully understood. It known that OLP has autoimmune features, and it suggested to be an disease. ELF-3 involved in differentiation keratinocytes deregulated different tumours diseases. CXCR-3 its ligands CXCL-10 CXCL-11 are increased diseases linked Th-1 immune response.To analyse compare expression ELF-3, CXCR-3, lesions controls whole microdissected epithelium.Tissue biopsies from 20 patients clinically...