A5083588888- RNA modifications and cancer
- Social and Educational Sciences
- Cancer, Lipids, and Metabolism
- Bioinformatics and Genomic Networks
- BRCA gene mutations in cancer
- Spectroscopy Techniques in Biomedical and Chemical Research
- Ferroptosis and cancer prognosis
- Cancer-related Molecular Pathways
- Male Breast Health Studies
- Spectroscopy and Chemometric Analyses
- Glutathione Transferases and Polymorphisms
- Cancer Genomics and Diagnostics
- Mechanisms of cancer metastasis
- Mass Spectrometry Techniques and Applications
- Lipoproteins and Cardiovascular Health
- Aquaculture Nutrition and Growth
- Computational Drug Discovery Methods
- DNA Repair Mechanisms
- Heat shock proteins research
- ATP Synthase and ATPases Research
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
- Cancer and Skin Lesions
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
Norwegian University of Science and Technology
2015-2024
Linköping University
2021
Lund University
2008-2015
Karolinska Institutet
1993-2015
Women's & Children's Health Research Institute
2015
Faculty (United Kingdom)
2015
Skåne University Hospital
2014
Swedish Defence Research Agency
2008-2013
University of Liverpool
2010
Biology of Infection
2010
Deficient hydroxylation of debrisoquine is an autosomal recessive trait that affects approximately 7% the Caucasian population. These individuals (poor metabolizers) carry deficient CYP2D6 gene variants and have impaired metabolism severely commonly used drugs. The opposite phenomenon also exists, certain metabolize drugs very rapidly, resulting in subtherapeutic plasma concentrations at normal doses. In present study, we investigated molecular genetic basis for ultrarapid debrisoquine....
The Toll/interleukin-1 receptor (TIR) domain is a highly conserved signaling found in the intracellular regions of Toll-like receptors (TLRs), interleukin-1 receptors, and several cytoplasmic adaptor proteins. TIR domains mediate signal transduction through recruitment proteins play critical roles innate immune response inflammation. This work presents 2.2A crystal structure human TLR10, revealing symmetric dimer asymmetric unit. interaction surface contains residues from BB-loop, DD-loop,...
The 70-kDa heat shock proteins (Hsp70) are chaperones with central roles in processes that involve polypeptide remodeling events. Hsp70 consist of two major functional domains: an N-terminal nucleotide binding domain (NBD) ATPase activity, and a C-terminal substrate (SBD). We present the first crystal structures four human isoforms, those NBDs HSPA1L, HSPA2, HSPA5 HSPA6. As previously family members, all crystallized closed cleft conformation, although slight opening through rotation...
Male breast cancer (MBC) is a rare and inadequately characterized disease. The aim of the present study was to characterize MBC tumors transcriptionally, classify them into comprehensive subgroups, compare with female (FBC). A total 66 clinicopathologically well-annotated fresh frozen were analyzed using Illumina Human HT-12 bead arrays, tissue microarray 220 constructed for validation immunohistochemistry. Two external gene expression datasets used comparison purposes: 37 MBCs 359 FBCs....
Accumulation and aggregation of misfolded proteins is a hallmark several diseases collectively known as proteinopathies. Autophagy has cytoprotective role in associated with protein aggregates. Age-related macular degeneration (AMD) the most common neurodegenerative eye disease that evokes blindness elderly. AMD characterized by retinal pigment epithelial (RPE) cells leads to loss photoreceptor central vision. The initial phase associates accumulation intracellular lipofuscin extracellular...
Ethanol-inducible CYP2E1 is an enzyme of major toxicological interest because it metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. has also been implicated in alcohol liver disease its contribution oxidative stress. Previously, polymorphic alleles with mutations introns the 5'-flanking regulatory region have described, their presence related incidence lung cancer. In present investigation, we investigated whether any functional are linked above-mentioned rare...
We report two crystal structures of the PARP domain human tankyrase-2 (TNKS2). Tankyrases are involved in fundamental cellular processes such as telomere homeostasis and Wnt signaling. The complex TNKS2 with potent inhibitor XAV939 provides insights into molecular basis strong interaction suggests routes for further development tankyrase inhibitors.
Abstract Standoff identification of explosives at distances up to 55 m has been performed by applying spontaneous Raman spectroscopy. This work focused on detection in a realistic environment, using an outdoors test field and performing experiments under varying weather conditions such as rain‐ or snowfall bright sunshine. The instrumentation, based 532 nm pulsed laser source combined with gated detection, proved the performance insensitive variations. Investigated HMEs precursors were TATP,...
Abstract Multiple myeloma (myeloma in short) is an incurable cancer of antibody-producing plasma cells that comprise 13% all hematological malignancies. The proteasome inhibitor bortezomib has improved treatment significantly, but inherent and acquired resistance to the drug remains a problem. We here show bortezomib-induced cytotoxicity was completely dampened when were supplemented with cysteine or its derivative, glutathione (GSH) ANBL-6 INA-6 cell lines. GSH major component antioxidative...
Inflammation is crucial in the defense against infections but must be tightly controlled to limit detrimental hyperactivation. Our diet influences inflammatory processes and omega-3 polyunsaturated fatty acids (n-3 PUFAs) have known anti-inflammatory effects. The balance of pro- coordinated by macrophages macroautophagy/autophagy has recently emerged as a cellular process that dampens inflammation. Here we report n-3 PUFA docosahexaenoic acid (DHA) transiently induces cytosolic speckles...
Background. Male breast cancer (MBC) is an uncommon disease and there limited information on the prognostic impact of routinely used clinicopathological parameters. Material methods. In a retrospective setting, we reviewed 197 MBC patients with accessible paraffin-embedded tumor tissue data. Immunohistochemical (IHC) stainings were performed microarrays histological grading conventional slides. Cox proportional regression models applied for uni- multivariate analyses using death as event....
The rapidly growing collection of diverse genome-scale data from multiple tumor types sheds light on various aspects the underlying biology. With objective to identify genes importance for breast tumorigenesis in men and enable comparisons with important cancer development women, we applied computational framework COpy Number EXpression In Cancer (CONEXIC) detect candidate driver among all altered passenger genes. Unique this approach is that each gene associated several modules are believed...
Abstract Background: Statins purportedly exert antitumoral effects, but the underlying mechanisms are currently not fully elucidated. The aim of this study was to explore potential statin-induced effects on global gene expression profiles in primary breast cancer. Experimental Design: This window-of-opportunity phase II trial enrolled 50 newly diagnosed cancer patients prescribed atorvastatin (80 mg/day) for 2 weeks presurgically. Pre- and posttreatment tumor samples were analyzed using...
The complete molecular basis of the organ-specificity metastasis is elusive. This study aimed to provide an independent characterization transcriptional landscape breast cancer metastases with specific objective identify liver metastasis-selective genes prognostic importance following primary tumor diagnosis.A cohort 304 women advanced was studied. Associations between site recurrence and clinicopathologic features were investigated. Fine-needle aspirates (n = 91) subjected whole-genome...
Abstract Background To our current understanding, solid tumors depend on suppressed local immune reactions, often elicited by the interaction between tumor cells and microenvironment (TME) components. Despite an improved understanding of anti-cancer responses in TME, it is still unclear how immuno-suppressive TME are formed some cancer survive metastasize. Methods identify major adaptations that undergo during development progression, we compared transcriptome proteome from metastatic 66cl4...
Cells degrade proteins either by proteasomes that clinically are targeted for example bortezomib or carfilzomib, formation of autophagosomes and lysosomal degradation can be inhibited hydroxychloroquine (HCQ). Multiple myeloma is unique among cancers because proteasomal inhibition has good clinical effects. However, some multiple patients display intrinsic resistance to the treatment most acquire over time. We hypothesized simultaneous targeting both arms protein could a way improve myeloma....
This paper gives a brief overview on our latest progress in the area of standoff detection. Standoff Raman measurements from 200 m and 470 distance have been performed bulk amounts TATP AN respectively, former through double sided window, latter under heavy rain. Resonance TNT, DNT NM vapors ppm concentration regime are presented, showing resonance enhancement range 2 (NM) to 57 000 (TNT) as compared 532 nm cross sections. Finally, application hyper spectral imaging is described, exemplified...
Paraplegin is an m-AAA protease of the mitochondrial inner membrane that linked to hereditary spastic paraplegias. The gene encodes FtsH-homology domain in tandem with AAA+ homology ATPase domain. protein believed form a hexamer uses ATPase-driven conformational changes its AAA-domain deliver substrate peptides We present crystal structure human paraplegin bound ADP at 2.2 A. This enables assignment roles specific side chains within catalytic cycle, and provides structural basis for...
The early B-cell factor (EBF) transcription factors are central regulators of development in several organs and tissues. This protein family shows low sequence similarity to other families, which is why structural information for the functional domains these proteins crucial understand their biochemical features. We have used a modular approach determine crystal structures structured EBF family. DNA binding domain reveals striking resemblance Rel homology superfamily but contains unique zinc...
Human purine de novo synthesis pathway contains several multi-functional enzymes, one of which, tri-functional GART, three enzymatic activities in a single polypeptide chain. We have solved structures two domains bearing separate catalytic functions: glycinamide ribonucleotide synthetase and aminoimidazole synthetase. Structures are compared with those homologous enzymes from prokaryotes analyzed terms the mechanism. also report small angle X-ray scattering models for full-length protein....