A5023579966- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Diseases and Treatments
- Education and Learning Interventions
- Supramolecular Self-Assembly in Materials
- Electrospun Nanofibers in Biomedical Applications
- Diverse Approaches in Healthcare and Education Studies
- Genomics, phytochemicals, and oxidative stress
- Retinal Development and Disorders
- Chemical Synthesis and Analysis
- Retinal and Optic Conditions
- Health and Wellbeing Research
- Nutrition, Health and Food Behavior
- Education, Safety, and Science Studies
- Wound Healing and Treatments
- Neurological Complications and Syndromes
- Mesenchymal stem cell research
- Click Chemistry and Applications
- Molecular Sensors and Ion Detection
- Nanopore and Nanochannel Transport Studies
- Graphene and Nanomaterials Applications
- RNA Interference and Gene Delivery
- Healthcare and Venom Research
- 3D Printing in Biomedical Research
- Chemical Synthesis and Reactions
- Angiogenesis and VEGF in Cancer
Johns Hopkins University
2013-2024
Johns Hopkins Medicine
2013-2024
Kunsan National University
2024
Smith-Kettlewell Eye Research Institute
2021
LG (South Korea)
2019
National Eye Institute
2018
University of Cincinnati Medical Center
2016
University of Cincinnati
2010-2014
Iowa State University
2009-2013
Sungkyunkwan University
2011
Controlled translocation of molecules and ions across lipid membranes is the basis numerous biological functions. Because synthetic systems can help researchers understand more complex ones, many chemists have developed mimics transporters. Both need to deal with similar fundamental challenges. In addition providing mechanistic insights into transport mechanisms, transporters are useful in a number applications including separation, sensing, drug delivery, catalysis.In this Account, we...
Retinal ischemia-reperfusion (I/R) is a pathophysiological process contributing to cellular damage in multiple ocular conditions, including glaucoma, diabetic retinopathy, and retinal vascular occlusions. Rodent models of I/R injury are providing significant insights into mechanisms treatment strategies for human injury, especially with regard neurodegenerative the neurovascular unit. Presented here protocol inducing mice through elevation intraocular pressure (IOP). In this protocol,...
Retinal ischemia results in neuronal degeneration and contributes to the pathogenesis of multiple blinding diseases. Recently, fumaric acid ester dimethyl fumarate (DMF) has been FDA-approved for treatment sclerosis, based on its neuroprotective anti-inflammatory effects. Its potential role as a agent retinal diseases received little attention. In addition, DMF's mode action remains elusive, although studies have suggested nuclear factor erythroid 2-related 2 (Nrf2) activation an important...
Retinal ischemia plays a critical role in multiple vision-threatening diseases and leads to death of retinal neurons, particularly ganglion cells. Oxidative stress an important this cell loss. Nrf2 (NF-E2-related factor 2) is major regulator the antioxidant response, its retina increasingly appreciated. We investigated potential neuroprotective function after ischemia-reperfusion (I/R) injury. In experimental model I/R, knockout mice exhibited much greater loss neuronal cells layer than...
High-refractive-index sulfur-rich polymers with significantly improved thermal properties are prepared using divinylbenzene (DVB) as a comonomer in modified, low-temperature inverse vulcanization elemental sulfur. Differential scanning calorimetry and Fourier transform infrared studies reveal that under the modified conditions, homopolymerized DVB segments form, leading to high glass-transition temperatures (Tg > 100 °C) stability previously unattainable from of bifunctional olefin...
Abstract Ischemic retinopathies including diabetic retinopathy are major causes of vision loss. Inner blood-retinal barrier (BRB) breakdown with retinal vascular hyperpermeability results in macular edema. Although dysfunction the neurovascular unit neurons, glia, and cells is now understood to underlie this process, there a need for fuller elucidation underlying events BRB ischemic disease, systematic analysis myeloid exploration cellular cross-talk. We used an approach microglia depletion...
Low-amplitude electric field (EF) is an important component of wound-healing response and can promote vascular tissue repair; however, the mechanisms action on endothelium remain unclear. We hypothesized that physiological amplitude EF regulates angiogenic microvascular endothelial cells via activation mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway. A custom set-up allowed non-thermal application high (7.5 GHz) low (60 Hz) frequency. Cell responses...
Retinal ischemia-reperfusion (I/R) is a pathophysiological process contributing to cellular damage in multiple ocular conditions, including glaucoma, diabetic retinopathy, and retinal vascular occlusions. Rodent models of I/R injury are providing significant insights into mechanisms treatment strategies for human injury, especially with regard neurodegenerative the neurovascular unit. Presented here protocol inducing mice through elevation intraocular pressure (IOP). In this protocol,...
Ischemic retinopathies are major causes of blindness worldwide. Local hypoxia created by loss vascular supply leads to tissue injury and aberrant neovascularization in the retina. There is a great need for therapies that enhance revascularization hypoxic neuroretinal tissue. To test therapeutic feasibility human-induced pluripotent stem cell–derived endothelial cells (hiPSC-ECs) treatment ischemic retinopathies, we compared angiogenic potential hiPSC-ECs with mature human retinal (HRECs)...
Diabetic retinopathy (DR) is the leading cause of blindness in working-age adults. Severe visual loss DR primarily due to proliferative diabetic retinopathy, characterized by pathologic preretinal angiogenesis driven retinal ischemia. Microglia, resident immune cells retina, have emerged as a potentially important regulator angiogenesis. Corticosteroids including triamcinolone acetonide (TA), known for their antiangiogenic effects, are used treating diseases, but use significantly limited...
Recently, intelligent wearable sensors applied in the field of smart sports have attracted much attention. Hence, we designed a pipe arrangement structure triboelectric nanogenerator (PA-TENG), and it can obtain mechanical energy monitor motion. The materials are composed polydimethylsiloxane layer attached to silicone tube nylon layer. conductive copper surface serves as electrode PA-TENG. Meanwhile, soft endow PA-TENG device with ability sense pressure. results indicate that this substrate...
Hydrophobic interactions normally are not considered a major driving force for self-assembling in hydrophobic environment. When macrocyclic oligocholates were placed within lipid membranes, however, the macrocycles pulled water molecules from aqueous phase into their hydrophilic internal cavities. These had strong tendencies to aggregate environment and templated self-assemble transmembrane nanopores. This counterintuitive effect resulted some highly unusual transport behavior. Cholesterol...
Oligocholate foldamers with different numbers and locations of guanidinium−carboxylate salt bridges were synthesized. The introduced by incorporating arginine glutamic acid residues into the foldamer sequence. conformations these studied fluorescence spectroscopy in homogeneous solution, anionic nonionic micelles, lipid bilayers. Environmental effects instead inherent foldability found to dominate folding. As noncovalent forces become involved molecules, best folder one environment could...
ABSTRACT Reduced mobilization of endothelial progenitor cells (EPCs) from the bone marrow (BM) and impaired EPC recruitment into wound represent a fundamental deficiency in chronic ulcers. However, mechanistic understanding role BM‐derived EPCs cutaneous neovascularization healing remains incomplete, which impedes development EPC‐based therapies. The objective this study was to determine both under normal conditions using single (EPC) or double‐deficiency (EPC + diabetes) models healing....
Ischemic retinopathies including diabetic retinopathy are major causes of blindness. Although neurons and Müller glia recognized as important regulators reparative pathologic angiogenesis, the role mononuclear phagocytes (MPs) — particularly microglia, resident retinal immune cells is unclear. Here, we found MP activation in human retinopathy, especially neovessels from neovascular membranes proliferative TNF-α expression. There was similar mouse oxygen-induced (OIR) model ischemia-induced...
Diabetic cardiomyopathy (DCM) is a diabetic complication, which results in myocardial dysfunction independent of other etiological factors. Abnormal intracellular calcium ([Ca2+]i) homeostasis has been implicated DCM and may precede clinical manifestation. Studies cardiomyocytes have shown that diabetes impaired [Ca2+]i due to altered sarcoplasmic reticulum Ca2+ ATPase (SERCA) sodium-calcium exchanger (NCX) activity. Importantly, also be involved diabetes-associated endothelial dysfunction,...
Macrocyclic oligocholates were found in a previous work (Cho, H.; Widanapathirana, L.; Zhao, Y. J. Am. Chem. Soc.2011, 133, 141−147) to stack on top of one another lipid membranes form nanopores. Pore formation was driven by strong tendency the water molecules interior amphiphilic macrocycles aggregate nonpolar environment. In this work, cholate oligomers terminated with guanidinium and carboxylate groups cause efflux hydrophilic such as glucose, maltotriose, carboxyfluorescein (CF) from...
Objective: The effect of chronic hyperglycemic exposure on endothelial cell (EC) phenotype, impaired wound neovascularization, and healing is not completely understood. hypotheses are: 1) to diabetic conditions in vivo impairs the angiogenic potential ECs 2) this deficiency can be improved by an extracellular microenvironment peptide nanofibers. Approach: Angiogenic microvascular isolated from (db/db) wild type (wt) mice was assessed quantifying migration, proliferation, apoptosis, capillary...