A5034331145- Modular Robots and Swarm Intelligence
- Micro and Nano Robotics
- Gene Regulatory Network Analysis
- Molecular Communication and Nanonetworks
- Nonlinear Dynamics and Pattern Formation
- Microfluidic and Bio-sensing Technologies
- Advanced Thermodynamics and Statistical Mechanics
- Advanced Materials and Mechanics
- Sperm and Testicular Function
- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Slime Mold and Myxomycetes Research
- Bacterial Genetics and Biotechnology
- Advanced Fluorescence Microscopy Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Stochastic processes and statistical mechanics
- Monoclonal and Polyclonal Antibodies Research
- Nanopore and Nanochannel Transport Studies
- Block Copolymer Self-Assembly
- Single-cell and spatial transcriptomics
- DNA and Biological Computing
- 3D Printing in Biomedical Research
- RNA and protein synthesis mechanisms
University of California, Santa Barbara
2021-2024
Weizmann Institute of Science
2014-2024
The assembly of artificial cells capable executing synthetic DNA programs has been an important goal for basic research and biotechnology. We assembled two-dimensional compartments fabricated in silicon as metabolism, programmable protein synthesis, communication. Metabolism is maintained by continuous diffusion nutrients products through a thin capillary, connecting synthesis the compartment with environment. programmed expression cycles, autoregulated levels, signaling gradient, equivalent...
Understanding how biochemical networks lead to large-scale nonequilibrium self-organization and pattern formation in life is a major challenge, with important implications for the design of programmable synthetic systems. Here, we assembled cell-free genetic oscillators spatially distributed system on-chip DNA compartments as artificial cells, measured reaction-diffusion dynamics at single-cell level up multicell scale. Using gene network programmed molecular interactions that control...
In microtubule-based active nematics, motor-driven extensile motion of microtubule bundles powers chaotic large-scale dynamics. We quantify the interfilament sliding both in isolated and a dense nematic. The extension speed an pair is comparable to molecular motor stepping speed. contrast, net 2D nematics significantly slower; speeds are widely distributed about average filaments exhibit contractile relative motion. These measurements highlight challenge connecting rate multimotor...
Direct electric-field manipulation of gene expression reactions would simplify the design biochemical networks by replacing complex biomolecular interactions with push-button operations. Here, we applied a localized electric field gradient at megahertz frequency to manipulate cell-free gene-expression reaction in DNA compartment on chip. We broke spatial symmetry homogeneous creating trap for macromolecules region maximal intensity 50 μm from immobilized DNA. Free regulation, demonstrated...
Significance Single-molecule techniques have elucidated how isolated molecular motors generate piconewton forces with unprecedented detail. However, in diverse biological and synthetic settings, force-generating proteins collectively power nonequilibrium dynamics, including continuous large-scale rearrangements persistent fluid flows. Characterizing motor-generated these dense dynamical environments remains a challenge. We assembled reversible DNA-based force-sensing probe that, by an...
The on-chip large-scale-integration of genetically programmed artificial cells capable exhibiting collective expression patterns is important for fundamental research and biotechnology. Here, we report a 3D biochip with 2D layout 1024 DNA compartments as on 5 × mm
Cilia and flagella play a crucial role in the development function of eukaryotes. The activity thousands dyneins is precisely regulated to generate flagellar motility. complex proteome (600+ proteins) architecture structural core flagella, axoneme, have made it challenging dissect functions different complexes, like regulatory machinery. Previous reports suggested that flagellum American eel sperm lacks many canonical axonemal complexes yet still motile. Here, we use cryo-electron tomography...
The design of artificial cell models based on minimal surface-bound transcription-translation reactions aims to mimic the compartmentalization facilitated by organelles and inner interfaces in living cells. Dense DNA brushes as localized sources RNA proteins serve synthetic operons that have recently proven useful for autonomous synthesis assembly cellular machines. Here, we studied ribosome a gene-expression reaction surface contact with macroscopic reservoir. We first observed accumulation...
Demixing of binary liquids is a ubiquitous transition, which explained using well-established thermodynamic formalism that requires equality intensive thermodynamics parameters across the phase boundaries. transitions also occur when fluid mixtures are driven away from equilibrium, for example, by external shear flow. Predicting demixing transition under non-equilibrium non-potential conditions remains, however, challenge. We drive liquid-liquid separation (LLPS) attractive DNA nanostar...
The on-chip large-scale-integration of genetically programmed artificial cells capable exhibiting collective modes is an important goal for fundamental research and technology. Here, we report assembly a 2D layout 1024 monolithic DNA compartments as on 5-millimeter square silicon chip. Homeostatic cell-free protein synthesis reactions driven by genetic circuits occur inside the compartments. We created reaction-diffusion system with 30x30 lattice interconnected thin capillaries diffusion...
Abstract Cilia and flagella play a crucial role in the development function of most eukaryotic organisms. The structural core flagellum, axoneme, is conserved eukaryotes careful regulation dynein motors within axoneme required for proper ciliary beating. sperm flagellum from American eel ( Anguilla rostrata ) has been shown to lack many canonical axonemal proteins, including radial spokes, central pair complex, possibly even outer row arms, presenting “minimal” flagellar system. Here, we...