A5027222083- RNA and protein synthesis mechanisms
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Fuel Cells and Related Materials
- Bacterial Genetics and Biotechnology
- Engineering and Material Science Research
- Genomics and Phylogenetic Studies
- Advanced Biosensing Techniques and Applications
- Machine Learning in Bioinformatics
- Modular Robots and Swarm Intelligence
- Thermal and Kinetic Analysis
- Advanced biosensing and bioanalysis techniques
- Photoreceptor and optogenetics research
- Monoclonal and Polyclonal Antibodies Research
- Bioinformatics and Genomic Networks
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
Massachusetts Institute of Technology
2021-2023
Weizmann Institute of Science
2020
ORT Israel
2017
Three-dimensional wireframe DNA origami have programmable structural and sequence features that render them potentially suitable for prophylactic therapeutic applications. However, their innate immunological properties, which stem from parameters including geometric shape cytosine-phosphate-guanine dinucleotide (CpG) content, remain largely unknown. Here, we investigate the immunostimulatory properties of 3D on TLR9 pathway using both reporter cell lines primary immune cells. Our results...
Ribosome biogenesis is an efficient and complex assembly process that has not been reconstructed outside a living cell so far, yet the most critical step for establishing self-replicating artificial cell. We recreated of Escherichia coli's small ribosomal subunit by synthesizing capturing all its proteins RNA on chip. Surface confinement provided favorable conditions autonomous stepwise new subunits, spatially segregated from original intact ribosomes. Our real-time fluorescence measurements...
The complex functions of neuronal synapses depend on their tightly interconnected protein network, and dysregulation is implicated in the pathogenesis autism spectrum disorders schizophrenia. However, it remains unclear how synaptic molecular networks are altered biochemically these disorders. Here, we apply multiplexed imaging to probe effects RNAi knockdown 16 autism- schizophrenia-associated genes simultaneous joint distribution 10 proteins, observing several composition phenotypes...
The design of artificial cell models based on minimal surface-bound transcription-translation reactions aims to mimic the compartmentalization facilitated by organelles and inner interfaces in living cells. Dense DNA brushes as localized sources RNA proteins serve synthetic operons that have recently proven useful for autonomous synthesis assembly cellular machines. Here, we studied ribosome a gene-expression reaction surface contact with macroscopic reservoir. We first observed accumulation...
The function of neuronal circuits, and its perturbation by psychoactive molecules or disease-associated genetic variants, is governed the interplay between synapse activity synaptic protein localization synthesis across a heterogeneous population. Here, we combine in situ measurement multiprotein compositions activation states, calcium traces glutamate spiking, local translation specific genes, same individual synapses. We demonstrate how this high-dimensional data enables identification...
ABSTRACT The complex functions of neuronal synapses in the central nervous system depend on their tightly interacting, compartmentalized molecular network hundreds proteins spanning pre- and post-synaptic sites. This biochemical is implicated pathogenesis autism spectrum disorders schizophrenia, with identified common synaptopathologies numerous risk genes associated synaptic function. However, it remains unclear how altered these disorders, whether effects are to distinct genetic...