A5102884514- Biofuel production and bioconversion
- Plant Gene Expression Analysis
- Catalysis for Biomass Conversion
- Lignin and Wood Chemistry
- Bioenergy crop production and management
North Carolina State University
2023-2025
The domestication of forest trees for a more sustainable fiber bioeconomy has long been hindered by the complexity and plasticity lignin, biopolymer in wood that is recalcitrant to chemical enzymatic degradation. Here, we show multiplex CRISPR editing enables precise woody feedstock design combinatorial improvement lignin composition properties. By assessing every possible combination 69,123 multigenic strategies 21 biosynthesis genes, deduced seven different genome targeting concurrent...
Climate change profoundly impacts the health, productivity, and resilience of forest ecosystems threatens sustainability products wood-based industries. Innovations to enhance tree growth, development, adaptation offer unprecedented opportunities strengthen ecosystem mitigate effects climate change. Here, we established a method for protoplast isolation, purification, CRISPR-Cas ribonucleoprotein (RNP) delivery in Pinus taeda Abies fraseri as step towards accelerating genetic improvement...