A5055771090- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Enzyme Catalysis and Immobilization
- Microbial Community Ecology and Physiology
- Anaerobic Digestion and Biogas Production
- Lignin and Wood Chemistry
- Protist diversity and phylogeny
- Metal Extraction and Bioleaching
- Bioeconomy and Sustainability Development
- Metalloenzymes and iron-sulfur proteins
- Building materials and conservation
- Corrosion Behavior and Inhibition
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Enzyme-mediated dye degradation
- Advanced Cellulose Research Studies
- Enzyme Structure and Function
- Electrocatalysts for Energy Conversion
- Insect symbiosis and bacterial influences
- Bacteriophages and microbial interactions
- Biochemical and Molecular Research
- 3D Printing in Biomedical Research
- Catalysis for Biomass Conversion
North Carolina State University
2019-2025
Cell Biotech (South Korea)
2020
The domestication of forest trees for a more sustainable fiber bioeconomy has long been hindered by the complexity and plasticity lignin, biopolymer in wood that is recalcitrant to chemical enzymatic degradation. Here, we show multiplex CRISPR editing enables precise woody feedstock design combinatorial improvement lignin composition properties. By assessing every possible combination 69,123 multigenic strategies 21 biosynthesis genes, deduced seven different genome targeting concurrent...
CtfAB from the extremely thermophilic bacterium, Thermosipho melanesiensis, has been used for in vivo acetone production up to 70°C. This enzyme tentatively identified as rate-limiting step, due its relatively low binding affinity acetate. However, existing kinetic and mechanistic studies on this are insufficient evaluate hypothesis. Here, analysis of purified recombinant T. melanesiensis showed that it a ping pong bi mechanism typical CoA transferases with Km values acetate acetoacetyl-CoA...
ABSTRACT Society relies heavily on chemicals traditionally produced through the refinement of fossil fuels. The conversion renewable biomass to value-added by microbes, particularly hyperthermophiles (T opt ≥80°C), offers a alternative this traditional approach. Herein, we describe engineering hyperthermophilic archaeon Pyrococcus furiosus , which grows optimally ) at 100°C, for sugar 1-propanol. This was accomplished constructing hybrid metabolic pathway consisting two native and seven...
The order Thermoanaerobacterales currently consists of fermentative anaerobic bacteria, including the genus Caldicellulosiruptor . are represented by thirteen species; all, but one, have closed genome sequences. Interest in these extreme thermophiles has been motivated not only their high optimal growth temperatures (≥70°C), also ability to hydrolyze polysaccharides including, for some species, both xylan and microcrystalline cellulose. species isolated from geographically diverse thermal...
Certain members of the family Sulfolobaceae represent only archaea known to oxidize elemental sulfur, and their evolutionary history provides a framework understand development chemolithotrophic growth by sulfur oxidation. Here, we evaluate oxidation phenotype species leverage comparative genomic transcriptomic analysis identify key genes linked Metabolic engineering obligate heterotroph
Renewable alternatives for nonelectrifiable fossil-derived chemicals are needed and plant matter, the most abundant biomass on Earth, provide an ideal feedstock. However, heterogeneous polymeric composition of lignocellulose makes conversion difficult. Lignin presents a formidable barrier to fermentation nonpretreated biomass. Extensive chemical enzymatic treatments can liberate fermentable carbohydrates from biomass, but microbial routes offer many advantages, including concomitant...
The production of volatile industrial chemicals utilizing metabolically engineered extreme thermophiles offers the potential for processes with simultaneous fermentation and product separation. An excellent target chemical such a process is acetone (Tb = 56°C), ideally produced from lignocellulosic biomass. Caldicellulosiruptor bescii (Topt 78°C), an extremely thermophilic fermentative bacterium naturally capable deconstructing fermenting lignocellulose, was to produce acetone. When pathway...
Biological conversion of lignocellulosic biomass is significantly hindered by feedstock recalcitrance, which typically assessed through an enzymatic digestion assay, often preceded a thermal and/or chemical pretreatment. Here, we assay 17 lines unpretreated transgenic black cottonwood (Populus trichocarpa) utilizing lignocellulose-degrading, metabolically engineered bacterium, Caldicellulosiruptor bescii. The poplar were incubation with C. bescii strain that solubilized and converted the...
Extremely thermophilic bacteria from the genus Caldicellulosiruptor can degrade polysaccharide components of plant cell walls and subsequently utilize constituting mono- oligosaccharides. Through metabolic engineering, ethanol other industrially important end products be produced. Previous experimental studies identified a variety carbohydrate-active enzymes in model species saccharolyticus bescii, while prior transcriptomic experiments their putative carbohydrate uptake transporters. We...
Metabolic modeling was used to examine potential bottlenecks that could be encountered for metabolic engineering of the cellulolytic extreme thermophile Caldicellulosiruptor bescii produce bio-based chemicals from plant biomass. The model utilizes subsystems-based genome annotation, targeted reconstruction carbohydrate utilization pathways, and biochemical physiological experimental validations. Specifically, transport pathways involving 160 genes their corresponding functions were...
Microbial deconstruction of lignocellulose for the production biofuels and chemicals requires hydrolysis heterogeneous hemicelluloses to access microcrystalline cellulose portion. This work extends previous in vivo vitro efforts characterize hemicellulose utilization by integrating genomic reconstruction, transcriptomic data, operon structures, biochemical characteristics key enzymes understand deployment functionality hemicellulases extreme thermophile Caldicellulosiruptor bescii .
The complete genome sequence of the extremely thermophilic bacterium Anaerocellum (f. Caldicellulosiruptor) danielii (DSM:8977) is reported here. A. a fermentative anaerobe and capable lignocellulose degradation with potential applications in biomass production chemicals fuels from renewable feedstocks.
The genome sequences of three extremely thermophilic, lignocellulolytic Caldicellulosiruptor species were closed, improving previously reported multiple-contig assemblies. All 14 classified spp. now have closed genomes. Genome closure will enhance bioinformatic analysis the species, including identification carbohydrate-active enzymes (CAZymes) and comparison against other microorganisms.
Caldicellulosiruptor species hold promise as microorganisms that can solubilize the carbohydrate portion of lignocellulose and subsequently convert fermentable sugars into bio-based chemicals fuels. Members genus have surface layer (S-layer) homology domain-associated glycoside hydrolases (SLH-GHs) mediate attachment to biomass well hydrolysis carbohydrates. bescii , most studied member genus, has only one SLH-GH.
A genome-scale metabolic model, encompassing a total of 623 genes, 727 reactions, and 865 metabolites, was developed for Pyrococcus furiosus, an archaeon that grows optimally at 100°C by carbohydrate peptide fermentation. The model uses subsystem-based genome annotation, along with extensive manual curation 237 gene-reaction associations including those involved in central carbon metabolism, amino acid energy metabolism. redox balance P. furiosus investigated through random sampling flux...
The platform chemical 2,3-butanediol (2,3-BDO) is used to derive products, such as 1,3-butadiene and methyl ethyl ketone, for the fuel production industries. Efficient microbial 2,3-BDO at industrial scales has not been achieved yet various reasons, including product inhibition host organisms, mixed stereospecificity in formation, dependence on expensive substrates (i.e., glucose). In this study, we explore engineering of a pathway
ABSTRACT The complete genome sequence of the thermoacidophilic archaeon Metallosphaera sedula (DSM 5348) is reported here. M. , originally isolated from a volcanic field in Italy, prolific iron-oxidizing with applications bioleaching sulfide minerals.
Reported here are complete genome sequences for two anaerobic, thermophilic bacteria isolated from wheat straw, i.e., the (hemi)cellulolytic Thermoclostridium stercorarium subspecies strain RKWS1 (3,029,933 bp) and hemicellulolytic Thermoanaerobacter species RKWS2 (2,827,640 bp). Discovery of indigenous thermophiles in plant biomass suggests that high-temperature microorganisms more ubiquitous than previously thought.