summary . The goal of this research was to study the safety and efficacy transfusing citrate‐phosphate‐adenine anticoagulant‐preservative (CPDA‐1) RBC stored for up 28 days reduce donor exposures in premature infants. A prospective randomized two‐group conducted with very low‐birth‐weight infants that received at least one transfusion during hospital stay. Neonates randomly assigned Group 1 (26 infants) were transfused CPDA‐1 days; those 2 3 days. Demographic transfusion‐related data...

The RHD gene is highly polymorphic and the existence of a large number alleles results in RhD variant phenotypes. genotyping has been used to distinguish normal D antigen from variants due limitations serologic methods. purpose this study was determine phenotypic frequency RhCE antigens investigate present samples with weak or D- phenotypes Brazilian blood donors. A total 2007 donors were phenotyped for D, C, c, E e antigens. Samples as genotyped by polymerase chain reaction-sequence...

The human neutrophil antigen-2 (HNA-2) is expressed on a subpopulation of neutrophils as most subjects present negative plus positive HNA-2 population neutrophils. number expressing variable and may increase in pregnancy, infections, myeloproliferative disorders after G-CSF. This study investigated the presence polymorphisms gene encoding (CD177) individuals presenting different patterns antigen expression determined association single nucleotide (SNPs) with heterogeneous expression.Flow...

Introdução: Reações transfusionais podem acarretar sérias consequências aos receptores de hemocomponentes. Objetivo: Analisar a proporção reações adversas quanto ao grau severidade observado nas transfusões sangue e identificar os fatores associados à gravidade dos incidentes imediatos. Método: Estudo longitudinal retrospectivo avaliação epidemiológica das imediatas ocorridas entre 2007-2019 em hospital ensino. Resultados: Das 332.222 sanguíneas administradas, foram reportadas 1.448...

Background and Objectives Benefits of adopting restrictive guidelines for erythrocyte transfusions are still controversial. The objective this study was to verify if a very strict guideline could reduce in preterm infants without adverse outcomes. Materials Methods Two prospective cohorts neonates with gestational age < 37 weeks birth weight 1500 g were studied. Neonates born Period 1 submitted transfusions. In 2, new stricter protocol introduced. Infants both periods compared regarding...

ABSTRACT Objective: To describe the hematological profile in cord blood of late preterm and term newborns compare indices according to sex, weight for gestational age type delivery. Methods: Cross-sectional study with a second-level maternity. Multiple gestation, chorioamnionitis, maternal or fetal hemorrhage, suspected congenital infection, 5-minute Apgar <6, malformations, Rh hemolytic disease were excluded. Percentiles 3, 5,10, 25, 50, 75, 90, 95 97 calculated both groups. Results:...

BACKGROUND: The FcγRIIA gene is expressed in two polymorphic forms, R131 and H131, which differ by the replacement of histidine arginine at position 131. FCGR3B ( FcγRIIIB ) exists allelic isoforms, known as FCGR3B1 FcγRIIIB‐NA1 FCGR3B2 FcγRIIIB‐NA2 ), nucleotides 141, 147, 227, 277, 349. An additional polymorphism SH antigen that associated with FCGR3B3 FcγRIIIB‐SH allele. STUDY DESIGN AND METHODS: By use a PCR allele‐specific primers, polymorphisms were determined among 263 unrelated...

BACKGROUND: The HNA‐4a (Mart) and HNA‐5a (Ond) antigens are polymorphic variants of α M (CD11b) L (CD11a) subunits the β 2 ‐integrin, associated with single nucleotide polymorphisms (SNP) leading to amino acid dimorphisms. has been linked alloimmune neonatal neutropenia, but clinical significance is unclear. STUDY DESIGN AND METHODS: Using a polymerase chain reaction (PCR) sequence‐specific primers, frequency among 121 Brazilian blood donors 114 Amazon Indians was determined. A...

Background HNA ‐3 antigens are the result of a rs2288904 single‐nucleotide polymorphism ( SNP ) in CTL 2, and ‐3a ‐3b variants encoded by guanine adenine at Nucleotide Position 461. Anti‐ involved severe transfusion‐related acute lung injury reactions neonatal alloimmune neutropenia. Since distribution system was unknown S outh A mericans, this study we determined frequency alleles B razilians. Study Design Methods DNA 500 blood donors, 120 X ikrin merindians, 74 J apanese individuals, 124...

Hemolysis may occur in 9% to 40% of patients after solid organ transplantation and be caused by the passenger lymphocyte syndrome (PLS).We have prospectively examined 217 kidney transplant recipients before (Day -1) (up Days +10, +20, +30) surgery. ABO-identical was performed 180 (82.9%) patients, while 37 (17.1%) individuals received ABO-compatible nonidentical grafts. Direct antiglobulin tests (DATs) were tube technique (polyspecific anti-human globulin [IgG + C3d]), positive DAT samples...

Neonatal alloimmune neutropenia results from maternal alloimmunization to human neutrophil antigens. The alloantibodies involved in neonatal are against antigens HNA-1a, HNA-1b, HNA-1c, HNA-1d, HNA-2, HNA-3a, HNA-4a, HNA-4b, and HNA-5a; however, date, antibodies specific HNA-3b have not been reported.Blood samples 10,000 unselected neonates were analyzed, resulting the selection of 88 neutropenic newborns (neutrophil count <1.5 × 109 /L) 83 mothers (three pairs twins one triplet). HNA-3...

BACKGROUND : Despite the fact that anemia is one of most striking clinical features visceral leishmaniasis (kala‐azar), factors involved in its pathogenesis are not fully understood. Although cause seen these patients often multifactorial, sequestration and destruction RBCs enlarged spleen, immune mechanisms, alterations RBC membrane permeability have been implicated. STUDY DESIGN AND METHODS To investigate whether with kala‐azar were coated IgG, blood samples 67 tested, prospectively,...

The HPA-15 (Gov) alloantigen is a biallelic co-dominant system on human platelets, and its allele HPA-15a HPA-15b differ by an A-->C single nucleotide polymorphism at 2108 of the coding sequence resulting in Tyr682Ser substitution mature CD109 glycoprotein. Employing polymerase chain reaction-restriction fragment length technique, we determined gene frequencies among 276 subjects distinct Brazilian ethnic groups including, 15 Caucasians, African Brazilians, Orientals, 106 Amazon Xikrin...

HLA antibodies passively transferred to transfused recipients may cause transfusion reactions such as transfusion-related acute lung injury (TRALI), but in many of the reported TRALI incidents, no white blood cell have been identified. We investigated whether a higher number anti-HLA would be detected donor's plasma by using method with potential sensitivity rate.Sera from 300 previously pregnant female donors were screened for solid-phase mixed-antigen assay (enzyme-linked immunosorbent...

Human neutrophil reactive antibodies may cause clinical disorders such as transfusion-related acute lung injury, febrile transfusion reactions, alloimmune neonatal neutropenia, immune neutropenia after stem cell transplantation, refractoriness to granulocyte transfusion, drug-induced and autoimmune neutropenia. Using the immunofluorescence test by flow cytometry, phenotypic frequencies of human alloantigens (HNA)-1a, -1b, -2, -3a -4a were determined in 100 healthy Brazilian persons....

The high homology and the inverted orientation of RHD RHCE may give rise to non-functional aberrant RH alleles. genotyping is used screen matched donors African descent patients. This study aimed define a strategy for testing variants in blood provide compatible units transfusion patients with haematological diseases.Samples from 132 [101 Sickle cell disease (SCD), 14 myelodysplastic syndrome (MDS), 17 acute myelogenous leukaemia (AML)] 198 Brazilian were studied. Major group alleles, RHD,...

Neonatal alloimmune neutropenia (NAIN) is caused by maternal alloimmunisation to fetal human neutrophil antigens (HNAs). This study investigated HNA/HLA alloantibodies involved with NAIN and identified the frequency of in Brazilian neonates. (neutrophil count < 1.5 × 109 /L) was samples from 10,000 unselected neonates, resulting 88 neutropenic newborns (NBs) their 83 mothers. Genotyping performed PCR-SSP (HNA-1/-4) PCR-RFLP (HNA-3/-5). Serologic studies were GAT (granulocyte agglutination...

Adult T-cell leukemia/lymphoma (ATLL) is a malignant proliferation of mature helper T lymphocytes,1 and caused by human T-lymphotropic virus type I (HTLV-I);2 an HTLV-I infection endemic in the Caribbean, south-western Japan, South America Africa.3,4 Seroepidemiological studies suggest that it also Brazil.5 Although carriers show polyclonal integration lymphocytes, only patients with ATLL various subtypes monoclonal tumor cells.6,7 Cutaneous lymphomas (CTCL) are group primary cutaneous...

BACKGROUND: The FCGR3B gene encoding the FcyRIIIb receptor for immunoglobulin G has three polymorphic forms known as HNA‐1a, HNA‐1b, and HNA‐1c, encoded by alleles * 01 , 02 03 respectively. It is not clear whether inheritance of allele, which encodes linked or to other two alleles. objective this study was identify pattern allele in Brazilians. STUDY DESIGN AND METHODS: Blood samples from nine families with at least one (+) member, totalizing 47 individuals, were studied. presence detected...

KELnull (K0) persons can produce clinically significant anti-KEL5 antibody after transfusion and/or pregnancy, requiring K0 blood when indicated. 37 alleles have been reported in studies over different populations, but none Amerindian-Caucasian descendants from South America. The aim of this study was to identify the molecular basis phenotype Brazilians.We investigated three samples Brazilian banks (Recife, Manaus, and Vila Velha) women with anti-KEL5. KEL antigen typing performed by...

The RHD gene is responsible for the expression of D antigen while RHCE encodes proteins carrying antigens C or c, and E e. present absent depending on RH haplotype, displays four common alleles (ce, Ce, cE, CE) two antithetical (allelic) series antigens. deletion leading cause Dphenotype worldwide.1 Functional (antigen) variations in Rh blood group system determined by insertion/deletion, single nucleotide polymorphisms (SNPs), conversion events genes. A weak type a variant RhD protein that...

Summary Autoimmune haemolytic anaemia (AIHA) is a rare clinical condition with immunoglobulin fixation on the surface of erythrocytes, or without complement activation. The pathophysiology AIHA complex and multifactorial, presenting functional abnormalities T B lymphocytes that generate an imbalance between lymphocyte activation, immunotolerance cytokine production culminates in autoimmune haemolysis. In AIHA, further laboratory data are needed to predict relapse refractoriness therapy,...