A5031658497- Advanced biosensing and bioanalysis techniques
- Silicon Nanostructures and Photoluminescence
- Biosensors and Analytical Detection
- Nanowire Synthesis and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Advanced Biosensing Techniques and Applications
- Analytical Chemistry and Sensors
- Bacterial Identification and Susceptibility Testing
- Microfluidic and Capillary Electrophoresis Applications
- Cancer Research and Treatments
- Gas Sensing Nanomaterials and Sensors
- Anodic Oxide Films and Nanostructures
- Molecular Junctions and Nanostructures
- Bacterial biofilms and quorum sensing
- Microfluidic and Bio-sensing Technologies
- Building materials and conservation
Technion – Israel Institute of Technology
2016-2023
Leibniz University Hannover
2017-2021
University of Haifa
2016
Aptamers and antibodies are compared as capture probes in a porous silicon-based optical biosensor for detection of target protein.
This work describes the design of optical aptamer-based porous silicon (PSi) biosensors for direct capture Lactobacillus acidophilus. Aptamers are oligonucleotides (single-stranded DNA or RNA) that can bind their targets with high affinity and specificity, making them excellent recognition elements biosensing applications. Herein, aptamer Hemag1P, which specifically important probiotic L. acidophilus, was utilized bacteria onto oxidized PSi Fabry-Pérot thin films. Monitoring changes in...
Abstract Microfluidic integration of biosensors enables improved biosensing performance and sophisticated lab-on-a-chip platform design for numerous applications. While soft lithography polydimethylsiloxane (PDMS)-based microfluidics are still considered the gold standard, 3D-printing has emerged as a promising fabrication alternative microfluidic systems. Herein, 3D-printed polyacrylate-based is integrated first time with label-free porous silicon (PSi)–based optical aptasensor via facile...
Porous silicon (PSi) nanomaterials have been widely studied as label-free optical biosensors for protein detection. However, these biosensors' performance, specifically in terms of their sensitivity (which is typically the micromolar range), insufficient many applications. Herein, we present a proof-of-concept application electrokinetic isotachophoresis (ITP) technique real-time preconcentration target on PSi biosensor. With ITP, highly concentrated zone delivered to sensing area, where...
The extensive and improper use of antibiotics has led to a dramatic increase in the frequency antibiotic resistance among human pathogens, complicating infectious disease treatments. In this work, method for rapid antimicrobial susceptibility testing (AST) is presented using microstructured silicon diffraction gratings integrated into prototype devices, which enhance bacteria-surface interactions promote bacterial colonization. microstructures act also as optical sensors monitoring growth...
The ultimate detection limit of optical biosensors is often limited by various noise sources, including those introduced the measurement setup. While sophisticated modifications to instrumentation may reduce noise, a simpler approach that can benefit all sensor platforms application signal processing minimize deleterious effects noise. In this work, we show applying complex Morlet wavelet convolution Fabry-P\'erot interference fringes characteristic thin film reflectometric effectively...
The anterior gradient homologue-2 (AGR2) protein is an attractive biomarker for various types of cancer. In pancreatic cancer, it secreted to the juice by premalignant lesions, which would be ideal stage diagnosis. Thus, designing assays sensitive detection AGR2 highly valuable potential early diagnosis and other Herein, we present a biosensor label-free investigate approaches enhancing aptasensor sensitivity accelerating target mass transfer rate reducing system noise. based on...
With new advances in infectious disease, antifouling surfaces, and environmental microbiology research comes the need to understand control accumulation attachment of bacterial cells on a surface. Thus, we employ intrinsic phase-shift reflectometric interference spectroscopic measurements silicon diffraction gratings non-destructively observe interactions between abiotic, microstructured surfaces label-free real-time manner. We conclude that combination specific material characteristics...
Abstract Among numerous approaches for treating cancer, clinically approved photodynamic therapy (PDT) is considered a promising non‐invasive therapeutic strategy solid tumors. While PDT has distinct advantages over conventional cancer treatments, systemic exposure to the photosensitizer and its stability are some of limitations clinical PDT. Herein, highly localized focal introduced based on direct biolistic delivery photosensitizer‐loaded carriers cancerous Degradable porous silicon...
Antimicrobial Susceptibility Testing In article number 2303285, Xin Jiang, Heidi Leonard, Ester Segal, and co-workers introduce the intensity-based phase-shift reflectometric interference spectroscopic measurements (iPRISM) device, capable of rapidly detecting urinary tract infections their antibiotic resistance by monitoring bacterial behavior on silicon diffraction gratings upon exposure to antibiotics. Coupling machine learning algorithm allows accurate prediction < 1 hour.
Photodynamic Therapy Highly localized photodynamic therapy (PDT) is achieved by biolistic delivery of photosensitizer-loaded porous silicon microparticles directly into solid tumors, as demonstrated Orit Shefi, Ester Segal, and co-workers in article 2300877. PDT irradiation following the uptake released photosensitizer payload induce substantial inhibition tumor growth vivo, opening new possibilities for an improved clinical treatment.
Integration of porous silicon optical biosensors with electrokinetic focusing allows for highly sensitive label-free detection target nucleic acids and proteins, showing a 1,000-fold improvement in limit compared to standard assays.