A5063344246- PI3K/AKT/mTOR signaling in cancer
- Sirtuins and Resveratrol in Medicine
- Cell Image Analysis Techniques
- HER2/EGFR in Cancer Research
- Image Processing Techniques and Applications
- Nuclear Receptors and Signaling
- Lung Cancer Treatments and Mutations
- Cancer-related Molecular Pathways
- Retinoids in leukemia and cellular processes
- Protein Degradation and Inhibitors
- PARP inhibition in cancer therapy
- RNA and protein synthesis mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Sarcoma Diagnosis and Treatment
- Autophagy in Disease and Therapy
- DNA Repair Mechanisms
- Malaria Research and Control
- Estrogen and related hormone effects
- Advanced Electron Microscopy Techniques and Applications
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Hepatitis B Virus Studies
- Polyamine Metabolism and Applications
Rockefeller University
2023
Tri-Institutional PhD Program in Chemical Biology
2023
Yeshiva University
2016-2018
Loss of TSC1 function, a crucial negative regulator mTOR signaling, is common alteration in bladder cancer. Mutations other members the PI3K pathway, leading to activation, are also found This provides rationale for targeting treatment cancer characterized by mutations and/or activation. In this study, we asked whether combination with rapamycin and resveratrol could be effective concurrently inhibiting signaling inducing cell death cells. rapamycin, was able block rapamycin-induced Akt...
The γ-tubulin ring complex (γ-TuRC) has essential roles in centrosomal and non-centrosomal microtubule organization during vertebrate mitosis. While there have been important advances understanding γ-TuRC-dependent nucleation, γ-TuRC capping of minus-ends remains poorly characterized. Here, we utilized biochemical reconstitutions cellular assays to characterize the human γ-TuRC's activity. Single filament showed that remained associated with a nucleated for tens minutes. In contrast, caps at...
Estrogen-related receptor alpha (ERRα) is an orphan nuclear factor that a master regulator of cellular energy metabolism. ERRα overexpressed in variety tumors, including ovarian, prostate, colorectal, cervical and breast, associated with more aggressive tumor worse outcome. In breast cancer, specifically, high expression increased rate recurrence poor prognosis. Because the common functions mTORC1/S6K1 signaling pathway regulation metabolism cancer pathogenesis, we focused on investigating...
Drug resistance is a challenge in anticancer therapy. In many cases, cancers can be resistant to the drug prior exposure, that is, possess intrinsic resistance. However, we lack target-independent methods anticipate cancer cell lines or characterize without priori knowledge of its cause. We hypothesized morphology could provide an unbiased readout To test this hypothesis, used HCT116 cells, mismatch repair-deficient line, isolate clones were sensitive bortezomib, well-characterized...
Approximately two thirds of all breast cancer cases are estrogen receptor (ER)-positive. The treatment this subtype with endocrine therapies is effective in the adjuvant and recurrent settings. However, their effectiveness compromised by emergence intrinsic or acquired resistance. Thus, identification new molecular targets can significantly contribute to development novel therapeutic strategies. In recent years, many studies have implicated aberrant levels translation initiation factors...
Homologous recombination (HR) is a conserved process that maintains genome stability and cell survival by repairing DNA double-strand breaks (DSBs). The RAD51-related family of proteins involved in repair DSBs; consequently, deregulation RAD51 causes chromosomal rearrangements stimulates tumorigenesis. RAD51C has been identified as potential tumor suppressor breast ovarian cancer susceptibility gene. Recent studies have also implicated estrogen DNA-damaging agent DSBs. We found ERα-positive...
Deregulated PI3K/mTOR signals can promote the growth of breast cancer and contribute to endocrine treatment resistance. This report aims investigate raptor its intracellular localization further understand role in ER-positive cancer. Raptor protein expression was evaluated by immunohistochemistry 756 primary tumors from postmenopausal patients randomized tamoxifen or no tamoxifen. In vitro, MCF7 cell line tamoxifen-resistant cells were studied track signaling changes upon resistance,...
ABSTRACT Drug resistance is a challenge in anticancer therapy, particularly with targeted therapeutics and cytotoxic compounds. In many cases, cancers can be resistant to the drug prior exposure, i.e., possess intrinsic resistance. However, we lack target-independent methods anticipate cancer cell lines or characterize without priori knowledge of its cause. We hypothesized that morphology could provide an unbiased readout sensitivity treatment. therefore isolated clonal were either sensitive...