A5030208569- Congenital heart defects research
- Ichthyology and Marine Biology
- Cardiac Fibrosis and Remodeling
- Physiological and biochemical adaptations
- Mechanical Circulatory Support Devices
- Coronary Artery Anomalies
- Cancer-related molecular mechanisms research
- Congenital Heart Disease Studies
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Fish biology, ecology, and behavior
University of Oxford
2018-2024
Although Astyanax mexicanus surface fish regenerate their hearts after injury, Pachón cave-dwelling counterparts cannot and, instead, form a permanent fibrotic scar, similar to the human heart. Myocardial proliferation peaks at levels in both and 1 week injury. However, Pachón, this peak coincides with strong scarring immune response, ultimately, cavefish cardiomyocytes fail replace scar. We identified lrrc10 be upregulated compared Similar cavefish, knockout of zebrafish impairs heart...
Runx1 is a transcription factor that plays key role in determining the proliferative and differential state of multiple cell types, during both development adulthood. Here, we report how specifically upregulated at injury site zebrafish heart regeneration, absence
A balanced immune response after cardiac injury is crucial to successful heart regeneration, but knowledge of what distinguishes a regenerative from scarring still limited. The Mexican cavefish provides unique comparative model study regeneration and within single species. Surface-dwelling fish are capable whereas their cave-dwelling counterparts lack this ability, similar the human heart. Using single-cell transcriptomics perturbations, we find significant differences in between two...
Regulation of heart size and shape is one the least understood processes in developmental biology. We have for first time analysed hearts Astyanax mexicanus identified several differences morphology between surface (epigean morph) cave-dwelling (troglomorph) morphs. Examination adult revealed that troglomorph possesses a smaller with rounder ventricle comparison to epigean morph. The appear arise early development, as 24 h post-fertilisation (hpf), while begin at 2 days post-fertilisation....
Reactivation of embryonic developmental pathways during regeneration aims to restore tissue architecture and functionality. We previously reported that following cryoinjury, a heterogeneous population Runx1-expressing endocardial cells differentially upregulates genes associate with scarring myofibroblast identity. Further analysis our published RNAseq data alongside 5 publicly available datasets now identifies additional heterogeneity in the Runx1-positive injured endocardium. Here, we show...
Runx1 is a transcription factor that plays key role in determining the proliferative and differential state of multiple cell-types, during both development adulthood. Here, we report how runx1 specifically upregulated at injury site zebrafish heart regeneration, but unexpectedly, absence results enhanced regeneration. Using single cell sequencing, found wild-type consists Runx1-positive endocardial cells thrombocytes induce expression smooth muscle collagen genes without differentiating into...