A5043671209- Biochemical and Molecular Research
- HER2/EGFR in Cancer Research
- Monoclonal and Polyclonal Antibodies Research
- Asymmetric Synthesis and Catalysis
- Synthesis and Catalytic Reactions
- HIV/AIDS drug development and treatment
- Photosynthetic Processes and Mechanisms
- Synthesis and Biological Evaluation
- Analytical Chemistry and Sensors
- Receptor Mechanisms and Signaling
- Ginseng Biological Effects and Applications
- Nanoparticle-Based Drug Delivery
- Plant biochemistry and biosynthesis
- Click Chemistry and Applications
- Adenosine and Purinergic Signaling
- Algal biology and biofuel production
- Microfluidic and Capillary Electrophoresis Applications
- Chemical Synthesis and Analysis
- Organ Transplantation Techniques and Outcomes
- Cancer Treatment and Pharmacology
- Natural product bioactivities and synthesis
- Drug Transport and Resistance Mechanisms
- Multicomponent Synthesis of Heterocycles
- Glycosylation and Glycoproteins Research
- Chromatography in Natural Products
Hunan Agricultural University
2024
Soil and Fertilizer Institute of Hunan Province
2024
Jinshan Hospital of Fudan University
2024
Northwest A&F University
2024
First Affiliated Hospital of Anhui Medical University
2024
Anhui Medical University
2024
Shenzhen University
2016-2023
Guangdong Institute for Drug Control
2011-2023
Yunnan Agricultural University
2021-2022
SUNY Upstate Medical University
2010-2022
The monoclonal antibody 2A4 binds an epitope derived from a cleavage site of serum amyloid protein A (sAA) containing -Glu-Asp- amino acid pairing. In addition to its reactivity with sAA deposits, the was also found bind fibrils composed immunoglobulin light chains. synthetic and human chain (AL) extracts high affinity even in presence soluble proteins. Immunohistochemistry biotinylated demonstrated positive reaction ALκ ALλ deposits various organs. Surface plasmon resonance analyses using...
Acute myeloid leukemia is a disorder characterized by abnormal differentiation of cells and clonal proliferation derived from primitive hematopoietic stem cells. Interventions that overcome have been shown to be promising therapeutic strategy for acute leukemia. In this study, we demonstrate CRISPR/Cas9-mediated knockout dihydroorotate dehydrogenase leads apoptosis normal cells, indicating potential regulator target in By screening library natural products, identified novel inhibitor,...
In this study, the ultra-high pressure extraction (UHPE) conditions for obtaining maximum flavonoid yield from Xinjiang jujube (Ziziphus jujuba Mill.) leaves (XJL) were optimized by response surface methodology (RSM). Box–Behnken design (BBD) was applied to evaluate effects of four variables (extraction temperature, pressure, time and liquid-to-solid ratio) on yield. The results showed that optimal (25.45 ± 0.21 mg/g) derived at 50.0 °C, 342.39 MPa, 11.56 min, a ratio 43.95 mL/g. Eight...
is a potential workhorse for production of various value-added chemicals including terpenoids, oleo-chemicals, and enzymes from low-cost feedstocks. However, the limited genetic toolbox hindering its metabolic engineering. In present study, four type I one novel II peroxisomal targeting signal (PTS1/PTS2) were characterized employed limonene first time in
The acetic acid catalyzed three-component reaction of pyrrolidine, aromatic aldehydes, and 3-arylideneoxindolin-2-ones in refluxing toluene afforded functionalized 7′-arylidenespiro[indoline-3,1′-pyrrolizines] good yields with high diastereoselectivity. similar 2-arylidene-1,3-indanediones also gave 7′-arylidenespiro[indene-2,1′-pyrrolizines] yields. However, the 3-phenacylideneoxindoles resulted a mixture spiro[indoline-3,1′-pyrrolizines] 7′-arylidene-substituted spirooxindoles moderate...
The multicomponent reaction of l-proline, isatins, and excess methyl propiolates in methanol unexpectedly afforded 2-(oxoindolin-3-ylidene)propylidene)pyrrolidin-1-yl)acrylates good yields, the products subsequently converted to unique nine-membered pyrrolo[1′,2′:1,9]azonino[6,5,4-cd]indoles presence p-TsOH. Furthermore, N-benzylisatins, alkyl refluxing chloroform unprecedentedly resulted eight-membered azocino[1,2-a]benzo[c][1,5]diazocines as main product. molecular structures...
Microalgae biofuel has become the most promising renewable energy over past few years. But limitations still exist because of its high cost. Although, efforts have been made in enhancement lipid productivity, major cost problem harvesting and oil extraction is intractable. Thus, idea fatty acids (FAs) secretion which can massively facilitate algae was investigated here.The cDNAs two long-chain acyl-CoA synthetases (LACSs) genes were cloned from Chlamydomonas reinhardtii named as cracs1...
A new type of azomethine ylides, which was <italic>in situ</italic> generated by the reaction ethyl glycinate hydrochloride and dimethyl acetylenedicarboxylate, reacted with 3-phenacylideneoxindoline-2-ones in ethanol to give polysubstituted spiro[indoline-3,3′-pyrrolidines] good yields.
A simple and efficient synthetic approach is reported for the synthesis of highly functionalized 4‐amino‐iminocoumarins 4 4‐aminoquinolines 6 in moderate to excellent yields, through a copper‐catalyzed multicomponent reaction. The reaction carried out one‐pot manner, using readily available terminal alkynes 1 , sulfonyl azides 2 2‐hydroxybenzonitriles 3 or 2‐aminobenzonitrile ( 5 ) as substrates at slightly elevated temperature under N . protocol operationally has good substrate scope....
The three-component reaction of 1,2,3,4-tetrahydroisoquinoline, isatins and 3-phenacylideneoxindoles in refluxing ethanol afforded dispiro[indoline-3,1′-pyrrolo[2,1-<italic>a</italic>]isoquinoline-3′,3′-indolines] (<bold>4a–4x</bold>) good yields <italic>via</italic> 1,3-dipolar cycloaddition.
Abstract Blocked cellular differentiation is a critical pathologic hallmark of acute myeloid leukemia (AML). Here, we showed that genetic activation the orphan GPCR GPR132 significantly induced cell AML both in vitro and vivo, indicating potential trigger differentiation. To explore therapeutic signaling, screened validated natural product 8-gingerol (8GL) as agonist. Notably, by 8GL promoted reduced colony formation human lines with diverse profiles. Mechanistic studies revealed treatment...
Abstract A new microflow injection analysis ( µ FIA) system on a chip coupled with chemiluminescence (CL) for the non‐enzymatic determination of uric acid is described. The FIA produced by using two transparent poly(methylmethacrylate) (PMMA) chips measured 50 × 40 5 mm, microchannels, etched CO 2 laser, were 200 µm wide and 100 deep, volume reaction area (RA) was about 1.2 µL. pump, accurate time control, monitored all reagents, including sample. sensed between luminol ferricyanide. linear...
A library of novel spirooxindole-pyrrolidine derivatives were facilely synthesized <italic>via</italic> 1,3-dipolar cycloaddition azomethine ylide generated from sarcosine and paraformaldehyde with various 3-methyleneoxindolines.