A5008769267- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Multiple Myeloma Research and Treatments
- Acute Lymphoblastic Leukemia research
- Viral-associated cancers and disorders
- Protein Degradation and Inhibitors
- Hematological disorders and diagnostics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Bone and Joint Diseases
- T-cell and B-cell Immunology
- Chronic Myeloid Leukemia Treatments
- Clinical Laboratory Practices and Quality Control
- Immune Cell Function and Interaction
- Lymphadenopathy Diagnosis and Analysis
- Epigenetics and DNA Methylation
- T-cell and Retrovirus Studies
- Glycosylation and Glycoproteins Research
- Cancer Treatment and Pharmacology
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Cancer Genomics and Diagnostics
- Melanoma and MAPK Pathways
- Cutaneous lymphoproliferative disorders research
- Histiocytic Disorders and Treatments
Vanderbilt University Medical Center
2011-2024
VA Tennessee Valley Healthcare System
2010-2024
Vanderbilt-Ingram Cancer Center
2020-2024
Breast Cancer Research Foundation
2020-2024
United States Department of Veterans Affairs
2011-2021
Veterans Health Administration
2021
Wayne State University
2019
Children's Hospital of Michigan
2019
University of Southern California
2019
Pharmacyclics (United States)
2019
Chronic lymphocytic leukemia (CLL) and small lymphoma (SLL) are essentially different manifestations of the same disease that similarly managed. A number molecular cytogenetic variables with prognostic implications have been identified. Undetectable minimal residual at end treatment chemoimmunotherapy or venetoclax-based combination regimens is an independent predictor improved survival among patients previously untreated relapsed/refractory CLL/SLL. The selection based on stage, presence...
We have purified to homogeneity from the myofibrillar fraction of pig bladder a mammalian heterotrimeric form PP-1, SMPP-1M. Purified SMPP-1M is similar in composition and substrate specificity avian gizzard PP-1M reported by Alessi et al. (Alessi, D., Macdougall, L. K., Sola, M. M., Ikebe, Cohen, P. (1992) Eur. J. Biochem. 210, 1023-1035) consists catalytic subunit PP-1 (37 kDa) two other equimolar subunits 130 20 kDa. The properties role its regulatory dephosphorylation myosin initiation...
Chronic lymphocytic leukemia (CLL) and small lymphoma (SLL) are characterized by a progressive accumulation of leukemic cells in the peripheral blood, bone marrow, lymphoid tissues. Treatment CLL/SLL has evolved significantly recent years because improved understanding disease biology development novel targeted therapies. In patients with indications for initiating treatment, selection treatment should be based on stage, patient's age overall fitness (performance status comorbid conditions),...
Chronic lymphocytic leukemia (CLL) is generally characterized by an indolent disease course. Histologic transformation (also known as Richter's transformation) to more aggressive lymphomas, such diffuse large B-cell lymphoma or Hodgkin lymphoma, occurs in approximately 2% 10% of patients and associated with a poor prognosis. These NCCN Guidelines Insights discuss the recommendations for diagnosis management histologic transformation.
The treatment landscape of chronic lymphocytic leukemia/small lymphoma (CLL/SLL) has significantly evolved in recent years. Targeted therapy with Bruton’s tyrosine kinase (BTK) inhibitors and BCL-2 emerged as an effective chemotherapy-free option for patients previously untreated or relapsed/refractory CLL/SLL. Undetectable minimal residual disease after the end is emerging important predictor progression-free overall survival treated fixed-duration inhibitor-based treatment. These NCCN...
Formation of major histocompatibility complex class I–associated peptides from membrane proteins has not been thoroughly investigated. We examined the processing an HLA-A*0201–associated epitope, YMDGTMSQV, that is derived protein tyrosinase by posttranslational conversion sequence YMNGTMSQV. Only YMDGTMSQV and YMNGTMSQV was presented HLA-A*0201 on cells expressing full-length tyrosinase, although both have similar affinities for are transported TAP. In contrast, translation in cytosol, as a...
In the Gleason grading system for prostatic cancer only two most prevalent patterns are reported, although a third (tertiary) pattern grade may be present. We compared pathologic stage of 227 radical prostatectomies with tertiary 5 to 604 lacking component. score 3 + 4 tumors were more likely present higher disease than those without component (P = 0.012) and at similar tumors. less organ-confined 0.02) have lymph node metastases 0.027). However, indistinguishable from The relative effects...
Autoimmune diseases often result from inappropriate or unregulated activation of autoreactive T cells. Traditional approaches to treatment autoimmune through immunosuppression have focused on direct inhibition In the present study, we examined targeted antigen-presenting cells as a means downregulate immune responses and treat disease. Dendritic (DCs) are central for initiation cell responses, including ones. A large portion DCs derived hematopoietic progenitors that express FLT3 receptor...
Hairy cell leukemia (HCL) is a rare type of indolent B-cell leukemia, characterized by symptoms fatigue and weakness, organomegaly, pancytopenia, recurrent opportunistic infections. Classic HCL should be considered distinct clinical entity separate from HCLvariant (HCLv), which associated with more aggressive disease course may not respond to standard therapies. Somatic hypermutation in the IGHV gene present most patients HCL. The BRAF V600E mutation has been reported classic but those other...
Treatment of adipocytes with insulin or phorbol 12-myristate 13-acetate (PMA) results in transient activation mitogen-activated protein kinase (MEK) (Tmax = 90 s) and (MAPK) 300 s). We have identified a novel insulin-stimulated MEK (I-MEKK) the 100,000 x g infranatant that shows rapid phasic kinetics temporally precede MEK. Maximal I-MEKK occurs within 20 +/- 5 s (S.D., n 3) followed by complete inactivation 30 10 3). was characterized anion-exchange gel filtration chromatography separated...
Abstract Although multiple components of the class I MHC processing pathway have been elucidated, participation nonproteasomal cytosolic enzymes has largely unexplored. In this study, we provide evidence for mechanisms in generation an HLA-A*0201-associated epitope from tyrosinase. This is presented two isoforms containing either Asn or Asp, depending on structure tyrosinase precursor. We show that deamidation to Asp dependent glycosylation endoplasmic reticulum (ER), and subsequent...
To address the overuse of testing that complicates patient care, diminishes quality, and increases costs by implementing diagnostic management team, a multidisciplinary system for development deployment guidelines hematologic malignancies.The team created evidence-based standard ordering protocols (SOPs) cytogenetic molecular were applied pathologists to bone marrow biopsy specimens on adult patients. Testing 780 performed during six months before SOP implementation was compared with 1,806...
Optimizing a clinical flow cytometry panel can be subjective process dependent on experience. We develop quantitative method to make this more rigorous and apply it B lymphoblastic leukemia/lymphoma (B-ALL) minimal residual disease (MRD) testing.We retrospectively analyzed our existing three-tube, seven-color B-ALL MRD used novel an optimized one-tube, eight-color panel, which was tested prospectively.The resulted in greater efficiency of time resources with no loss diagnostic...
ABSTRACT Idiopathic multicentric Castleman disease is a rare lymphoproliferative disorder that can be potentially fatal without timely diagnosis and treatment. We describe the first-ever reported occurrence of idiopathic with thrombocytopenia, anasarca, fever, reticulin fibrosis/renal insufficiency, organomegaly syndrome in liver transplant recipient. The was challenging as clinical presentation closely mimicked decompensated cirrhosis, profound thrombocytopenia limited tissue diagnosis....
Pathologists grade follicular lymphoma (FL) cases by selecting 10, random high power fields (HPFs), counting the number of centroblasts (CBs) in these HPFs under microscope and then calculating average CB count for whole slide. Previous studies have demonstrated that there is inter-reader variability among pathologists using this methodology grading.The objective study was to explore if newly available digital reading technologies can reduce variability.IN THIS STUDY, WE CONSIDERED THREE...
Context.—Infection is a common cause of lymphadenopathy in children and has numerous microbial etiologies. Lymph node biopsy considered keystone arriving at definite diagnosis. An accurate differential diagnosis from lymph can expedite minimize ancillary testing. Objective.—To assess compare the histopathologic epidemiologic features uncommon pediatric bacterial lymphadenitis. Design.—We searched our database for surgical specimens that had positive identification bacteria during an 8-year...
Fluorescence in situ hybridization for abnormalities common to myelodysplastic syndrome (MDS FISH) is often used with traditional karyotype the diagnosis and monitoring of myeloid neoplasms. However, its value these roles has been questioned. To evaluate utility, we compared MDS FISH results 544 bone marrow specimens obtained (180 cases) or follow-up (364 neoplasia. We found excellent concordance between karyotype, such that rarely abnormal (1.7% at 3.0% follow-up) cases normal karyotype....