A5073394279- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- ATP Synthase and ATPases Research
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Microfluidic and Bio-sensing Technologies
- RNA modifications and cancer
- Autophagy in Disease and Therapy
- Phagocytosis and Immune Regulation
- Escherichia coli research studies
- Ubiquitin and proteasome pathways
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Immune Cell Function and Interaction
- Cell Image Analysis Techniques
- Microtubule and mitosis dynamics
- Apelin-related biomedical research
- Hepatitis B Virus Studies
- Streptococcal Infections and Treatments
- Molecular Biology Techniques and Applications
- Trace Elements in Health
Max Planck Unit for the Science of Pathogens
2018-2025
Inserm
2022-2025
Université de Bordeaux
2011-2025
Régulations Naturelle et Artificielle
2023-2025
ARNA - Acides nucléiques: Régulations naturelles et artificielles
2025
Centre National de la Recherche Scientifique
2011-2024
Humboldt-Universität zu Berlin
2018-2024
Charité - Universitätsmedizin Berlin
2016-2024
Bordeaux Population Health
2024
Institut Européen de Chimie et Biologie
2020-2023
The bacterial flagellum is a self-assembling nanomachine. external flagellar filament, several times longer than cell body, made of few tens thousands subunits single protein: flagellin. A fundamental problem concerns the molecular mechanism how grows outside cell, where no discernible energy source available. Here, we monitored dynamic assembly individual flagella using in situ labelling and real-time immunostaining elongating filaments. We report that rate growth, initially ∼1,700 amino...
Many bacteria move using a complex, self-assembling nanomachine, the bacterial flagellum. Biosynthesis of flagellum depends on flagellar-specific type III secretion system (T3SS), protein export machine homologous to machinery virulence-associated injectisome. Six cytoplasmic (FliH/I/J/G/M/N) and seven integral-membrane proteins (FlhA/B FliF/O/P/Q/R) form flagellar basal body are involved in transport building blocks across inner membrane proton motive force-dependent manner. However, how...
The flagellum is the most complex macromolecular structure known in bacteria and composed of around two dozen distinct proteins. main building block long, external flagellar filament, flagellin, secreted through type-III secretion system at a remarkable rate several tens thousands amino acids per second, significantly surpassing rates achieved by other pore-based protein systems. evolutionary implications potential benefits this high for assembly function, however, have remained elusive. In...
Bax-dependent mitochondrial permeabilization during apoptosis is controlled by multiple factors, including the phosphorylation protein kinase AKT. We used heterologous co-expression of human Bax and AKT1 in yeast to investigate how modulates different steps underlying activation. found that AKT activated increased its cellular content. Both effects were dependent on Ser184, but a this residue did not fully explain Additional experiments with mutants substituted Ser184 suggested regulation...
RNA degradation is an essential process that allows bacteria to control gene expression and adapt various environmental conditions. It usually initiated by endoribonucleases (endoRNases), which produce intermediate fragments are subsequently degraded exoribonucleases (exoRNases). However, global studies of the coordinated action these enzymes lacking. Here, we compare targetome endoRNase Y with targetomes 3'-to-5' exoRNases from Streptococcus pyogenes, namely, PNPase, YhaM, RNase R. We...
During assembly of the bacterial flagellum, protein subunits that form exterior structures are exported through a specialized secretion apparatus energized by proton gradient. This category transport, together with similar process occurs in injectisomes gram-negative pathogens, is termed type-III secretion. The membrane-embedded part flagellar export contains five essential proteins: FlhA, FlhB, FliP, FliQ and FliR. Here, we have undertaken variety experiments support proposal...
The heterologous expression of Bax, and other Bcl-2 family members, in the yeast Saccharomyces cerevisiae, has proved to be a valuable reporter system investigate molecular mechanisms underlying their interaction with mitochondria. By combining co-expression Bax Bcl-xL mutants analyzes localization mitochondria post-mitochondrial supernatants, we showed that ability interact is dependent both on phosphorylation - mimicked by substitution S184D localization. This, previous data, provide basis...
Many bacteria swim through liquids or crawl on surfaces by rotating long appendages called flagella. Flagellar filaments are assembled from thousands of subunits that exported a narrow secretion channel and polymerize beneath capping scaffold at the tip growing filament. The assembly flagellum uses significant proportion biosynthetic capacities cell with each filament constituting ~1% total protein. Here, we addressed question whether flagellar can form new cap resume growth after breakage....
Significance To cope with harsh environments and cause infection, bacteria need to constantly adjust gene expression. Ribonucleases (RNases) control the abundance of regulatory protein-coding RNA through degradation maturation. The current characterization 3′-to-5′ exoribonucleases (exoRNases), processing RNAs from their 3′ end, is solely based on description a limited number targets processed by these RNases. Here, we characterized bacterial exoRNase targetomes. We show that YhaM,...
The BCL-2 (B cell CLL/Lymphoma) family is comprised of approximately twenty proteins that collaborate to either maintain survival or initiate apoptosis1. Following cellular stress (e.g., DNA damage), the pro-apoptotic effectors BAK (BCL-2 antagonistic killer 1) and/or BAX associated X protein) become activated and compromise integrity outer mitochondrial membrane (OMM), though process referred as permeabilization (MOMP)1. After MOMP occurs, cytochrome c) gain access cytoplasm, promote...
Type-III secretion systems (T3SSs) of the bacterial flagellum and evolutionarily related injectisome are capable translocating proteins with a remarkable speed several thousand amino acids per second. Here, we investigate how T3SSs able to transport at such high rate while preventing leakage small molecules. Our mutational evolutionary analyses demonstrate that an ensemble conserved methionine residues cytoplasmic side T3SS channel create deformable gasket (M-gasket) around fast-moving...
BAX physically interacts with TOM40 by anti bait coimmunoprecipitation (View interaction)