A5087385604- Antimicrobial Resistance in Staphylococcus
- RNA and protein synthesis mechanisms
- Pneumocystis jirovecii pneumonia detection and treatment
- Neonatal Health and Biochemistry
- Clinical Nutrition and Gastroenterology
- Neonatal and Maternal Infections
- Reproductive System and Pregnancy
- Reproductive tract infections research
- Fungal Infections and Studies
- Acute Kidney Injury Research
- Streptococcal Infections and Treatments
- Pneumonia and Respiratory Infections
- Pediatric health and respiratory diseases
- Tissue Engineering and Regenerative Medicine
- Bacterial biofilms and quorum sensing
- Pediatric Urology and Nephrology Studies
- Clostridium difficile and Clostridium perfringens research
- Antifungal resistance and susceptibility
Columbia University
2012-2015
University of Minho
2010-2015
α–Intercalated cells (A-ICs) within the collecting duct of kidney are critical for acid-base homeostasis. Here, we have shown that A-ICs also serve as both sentinels and effectors in defense against urinary infections. In a murine tract infection model, bound uropathogenic E. coli responded by acidifying urine secreting bacteriostatic protein lipocalin 2 (LCN2; known NGAL). A-IC–dependent LCN2 secretion required TLR4, mice expressing an LPS-insensitive form TLR4 expressed reduced levels...
The strong epidemiological association between cigarette smoke (CS) exposure and respiratory tract infections is conventionally attributed to immunosuppressive irritant effects of CS on human cells. Since pathogenic bacteria such as Staphylococcus aureus are members the normal microbiota reside in close proximity nasopharyngeal cells, we hypothesized that bioactive components might affect these organisms potentiate their virulence. Using a model organism, observed presence increased both...
Group B Streptococcus (GBS; agalactiae) is a major human pathogen that disproportionately affects neonates and women in the peripartum period an emerging cause of infection older adults. The primary toxin GBS, β-hemolysin/cytolysin (βH/C), has well-defined role pathogenesis invasive disease, but its urinary tract (UTI) unknown. Using both vitro vivo models, we analyzed importance βH/C GBS uropathogenesis. There were no significant differences bacterial density from bladders or kidneys mice...
Gardnerella vaginalis, the bacterial species most frequently isolated from women with vaginosis (BV), produces a cholesterol-dependent cytolysin (CDC), vaginolysin (VLY). At sublytic concentrations, CDCs may initiate complex signaling cascades crucial to target cell survival. Using live-cell imaging, we observed rapid formation of large membrane blebs in human vaginal and cervical epithelial cells (VK2 HeLa cells) exposed recombinant VLY toxin cell-free supernatants growing liquid cultures...
The incidence of Candida albicans infections and the relapse episodes after antifungal treatment have increased in recent decades. Recurrences are mainly due to persistence original infecting strain that may present genetic genomic rearrangements during interaction with host, reflecting adaptation. In this study, four isolates recovered from a patient recurrent candidemia were genotyped by microsatellite length polymorphism (MLP) multilocus sequence typing (MLST) found be variants same...
The contribution of specific factors to bacterial virulence is generally investigated through creation genetic “knockouts” that are then compared wild-type strains or complemented mutants. This paradigm useful understand the effect presence vs. absence a gene product but cannot account for concentration-dependent effects, such as may occur with some toxins. In order assess threshold and dose-response effects factors, robust systems tunable expression required. Recent evidence suggests...
The JCI, with the agreement of corresponding authors and coauthors, is retracting article "Denitrosylation HDAC2 by targeting Nrf2 restores glucocorticosteroid sensitivity in macrophages from COPD patients."The IP: streptavidin/IB: anti-HDAC2 immunoblot panel Figure 3B was recently identified as an inverted image right four lanes anti-H4 acetyl CHIP gel presented 2A.Additionally, anti-streptavidin/IB: anti-DDK Supplemental 3A left 2A.Further, 9B, incorrect immuno blot presented.