A5064075548- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Soil Carbon and Nitrogen Dynamics
- Protist diversity and phylogeny
- Atmospheric and Environmental Gas Dynamics
- Wastewater Treatment and Nitrogen Removal
- Microbial metabolism and enzyme function
- Bacteriophages and microbial interactions
- Climate change and permafrost
- Peatlands and Wetlands Ecology
- Marine and coastal ecosystems
- Anaerobic Digestion and Biogas Production
- Microbial Fuel Cells and Bioremediation
- Geology and Paleoclimatology Research
- Genomics and Phylogenetic Studies
- Isotope Analysis in Ecology
- Nematode management and characterization studies
- Environmental DNA in Biodiversity Studies
- Soil and Water Nutrient Dynamics
- Geochemistry and Elemental Analysis
- Legume Nitrogen Fixing Symbiosis
- Gut microbiota and health
- Legionella and Acanthamoeba research
- Agriculture, Soil, Plant Science
- Soil and Unsaturated Flow
Nagoya University
2016-2025
Kasetsart University
2024-2025
Institute of Fruit Tree and Tea Science
2021
Hokkaido University
2018
Max Planck Institute for Terrestrial Microbiology
2006-2007
University of Shiga Prefecture
2000-2006
Horiba (Japan)
1998
Reduced fertilizer usage is one of the objectives field management in pursuit sustainable agriculture. Here, we report on shifts bacterial communities paddy rice ecosystems with low (LN), standard (SN), and high (HN) levels N application (0, 30, 300 kg ha−1, respectively). The LN had received no for 5 years prior to experiment. HN plants showed a 50% decrease 60% increase biomass compared SN plant biomass, respectively. Analyses 16S rRNA genes suggested between root microbiomes, which were...
Summary Methane oxidation is a key process controlling methane emission from anoxic habitats into the atmosphere. Methanotrophs, responsible for aerobic oxidation, do not only oxidize but also assimilate methane. Once assimilated, carbon may be utilized by other organisms. Here we report on microbial food web in rice field soil driven A thin layer of water‐saturated was incubated under opposing gradients oxygen and 13 C‐labelled Bacterial eukaryotic communities incorporating were analysed...
Rice grain yield prediction with UAV-driven multispectral images are re-emerging interests in precision agriculture, and an optimal sensing time is important factor. The aims of this study were to (1) predict rice by using the estimated aboveground biomass (AGB) leaf area index (LAI) from vegetation indices (VIs) (2) determine estimating AGB LAI VIs for prediction. An experimental trial was conducted 2020 2021, involving two fertility conditions five japonica cultivars (Aichinokaori, Asahi,...
Photosynthesis by terrestrial vegetation is the driving force of carbon cycling between soil and atmosphere. The microbiota, decomposers organic matter, second player carrying out cycling. Numerous efforts have been made to quantify rhizodeposition respiration understand predict However, there few attempts link directly microbial community plant photosynthesis. We carried a pulse-chase labeling experiment in wetland rice system which plants various ages were labeled with (13)CO(2) for 6 h...
ABSTRACT Flooded rice fields have become a model system for the study of soil microbial ecology. In Italian fields, in particular, aspects from biogeochemistry to molecular ecology been studied, but impact protistan grazing on structure and function prokaryotic community has not examined yet. We compared an untreated control with γ-radiation-sterilized that had reinoculated natural bacterial assemblage. order verify observed effects were due did result sterilization, we set up third...
Biological methane oxidation is a key process in the cycle of wetland ecosystems. The methanotrophic biomass may be grazed by protozoa, thus linking to soil microbial food web. In present study, edibility different methanotrophs for protozoa was compared. number methanotroph-feeding rice field estimated determining most-probable (MPN) using as bacteria; naked amoebae and flagellates were dominant protozoa. Among ten strains examined source, seven yielded comparable with yield Escherichia...
Methane oxidation was studied in mesotrophic lake water (Lake Biwa, Japan) under thermally stratified conditions. rates at situ concentrations were very low from the epilimnion and thermocline but high hypolimnetic water. Incubation light conditions ranging 4.1 to 57 µmol photons m−2 s−1 resulted decreased methane This inhibition more severe as intensity increased. Addition of inorganic nitrogen (ammonium nitrate) did not promote inhibited it hypolimnion. activity observed after 1 month...
Abstract. The rapid expansion of rubber cultivation, driven by the demand for natural in tire industry constitutes a significant land-use change Southeast Asia. This has reduced soil methane (CH4) uptake, thereby weakening atmospheric CH4 removal over extensive areas. While fertilization is widespread practice plantations, its role further sink remained poorly understood. Over 1.5 years, we measured fluxes biweekly an experimental plantation with four distinct treatments to evaluate their...
Abstract The effects of the application level rice straw and percolation rate on leaching amounts methane, total water soluble organic carbon (TOC), hexose, Fe2+, Mn2+ with percolating were investigated in a soil column experiment. amount methane leachate was larger as (0.3–1.0 × 10-2 kg kg-1) increased. concentration reached saturation around 18th day after incubation 0.6 1.0 kg-1 treatments, remained at same afterwards. In treatments rates 0.26, 0.52, cm/d, did not change. percolated...
The microbial decomposition of plant residue is a central part the carbon cycle in soil ecosystems. Here, we explored microeukaryotic community responsible for uptake rice field through DNA-based stable-isotope probing (SIP) using dried callus labelled with (13) C as model substrate. Molecular fingerprinting PCR-DGGE showed that total eukaryotic under drained (upland) conditions distinctly changed within 3 days after was applied and stable thereafter. predominant group eukaryotes...
Abstract To estimate the impact of water percolation on nutrient status in paddy fields, seasonal variations concentrations cations, anions, inorganic carbon (IC), and dissolved organic (DOC) percolating that was collected from just below plow layer (PW-13) drainage pipes at 40 em depth (PW-40), as well irrigation were measured an irrigated field. Total amounts Ca, Mg, K, Fe, Mn leached PW-13 during period rice cultivation estimated to range about 390 770, 65 130, 33 66, 340 680, 44 87 kg...
Abstract Oxidation of methane and total water soluble organic carbon (TOC) in the subsoil, which percolated from plow layer, was investigated a column experiment. The amounts both TOC leachate decreased by percolation subsoil. Fe2+ layer nearly completely retained decomposition subsoil considered to result coupling with formation Fe2+. Methane estimated contribute ca. 19–21% amount formed materials leachate.
Aerobic methane-oxidizing bacteria (MOB) play an important role in mitigating methane emissions from paddy fields. In this study, we developed a differential quantification method for the copy number of pmoA genes type Ia, Ib, and IIa MOB field soil using chip-based digital PCR. Three probes specific to worked well PCR when genomic DNA isolates PCR-amplified fragments were examined as templates. When surface layer flooded quantified by PCR, numbers 105 -106 , 107 copies g-1 dry soil,...