To date, aggregation of DNA-functionalized gold nanoparticles by hybridization target DNA in a cross-linking configuration has been intensively studied. Here, we report that non-cross-linking is also possible and even better from the viewpoint genetic analysis because its speed sensitivity. In this system, 15 nm diameter functionalized with (alkanethiol)-15mer are hybridized to 15mer at room temperature. At high NaCl concentration (>/=0.5 M), complementary induces nanoparticle based on...

An extremely simple, power-free pumping method for poly(dimethylsiloxane) (PDMS) microfluidic devices is presented. By exploiting the high gas solubility of PDMS, energy pre-stored in degassed bulk therefore no additional structures other than channels and reservoirs are required. In a Y-shaped microchannel with cross section 100 µm width × 25 height, this has provided flow rate 0.5–2 nL s−1, corresponding to linear velocity 0.2–0.8 mm good reproducibility. As an application pumping, gold...

Miniaturization of chemical or biochemical systems creates extremely efficient devices exploiting the advantages microspaces. Although they are often targeted for implanted tissue engineered organs drug-delivery because their highly integrated systems, microfluidic usually powered by external energy sources and therefore difficult to be used in vivo. A device without need stimuli is needed. Previously, we demonstrated concept a cardiomyocyte pump using only input cells as driver (Yo Tanaka,...

We developed a microfluidic model of microcirculation containing both blood and lymphatic vessels for examining vascular permeability. The designed device harbors upper lower channels that are partly aligned separated by porous membrane, on this endothelial cells (BECs) (LECs) were cocultured back-to-back. At cell-cell junctions BECs LECs, claudin-5 VE-cadherin detected. permeability coefficient measured here was than the value reported isolated mammalian venules. Moreover, our results...

We developed a microsystem for cell experiments consisting of scanning thermal lens microscope detection system and culture microchip. The microchip was good liquid control in microspace, this results secure stimulation coincident vivo observation the responses. could detect nonfluorescent biological substances with extremely high sensitivity without any labeling materials had spatial resolution approximately 1 microm. This applied to monitoring cytochrome c distribution neuroblastoma-glioma...

Aggregation of DNA-modified gold nanoparticles in a non-cross-linking configuration has extraordinary selectivity against terminal mismatch the surface-bound duplex. In this paper, we demonstrate utility for detection single-base substitutions. The samples were prepared through standard protocols: DNA extraction, PCR amplification and primer extension. Oligonucleotide-modified correctly responded to unpurified products from extension: aggregation full match dispersion all mismatches....

The sensitive detection and quantification of DNA targets in the food industry environmental clinical settings are issues utmost importance ensuring contamination-free food, monitoring environment, battling disease. Selective probes coupled with powerful amplification techniques therefore major interest. In this study, we set out to create an integrated microchemical chip that benefits from microfluidic technology terms sensitivity a strong methodology provided jointly by padlock rolling...

This paper presents a simple fluid handling technique for microchip immunoassay. Necessary solutions were sequentially injected into microchannel by air-evacuated poly(dimethylsiloxane), and passively regulated capillary force at the inlet opening. For heterogeneous immunoassay, microchips are potentially useful reduction of sample consumption assay time. However, most previously reported have limitations in their use because needs external power sources handling. In this paper, an on-chip...

We have developed a novel, practical micro-ELISA system for sensitive and rapid allergy diagnosis. The enzymatic reactions occurred under stopped-flow conditions, resulting in both high precision sensitivity. A BSA-biotin-avidin linker was introduced the immobilization of water-soluble allergens on polystyrene microbeads, enabling sufficient density to provide Evaluation system's performance showed good detection limit (2 ng/mL) total IgE measurement. In addition, correlation with...

Microfluidic-based cell-stretching devices are vital for studying the molecular pathways involved in cellular responses to mechanobiological processes. Accurate evaluation of these requires detailed observation cells cultured this device. This study aimed develop a method preparing microscope slides enable high-magnification imaging devices. The key innovation is creating peelable bond between cell culture membrane and upper channel, allowing easy removal layer precise cutting microscopy....

Molecular networks of organelle membranes are involved in many cell processes. However, the nature plasma membrane as a barrier to various analytical tools, including antibodies, makes it challenging examine intact without affecting their structure and functions via permeabilization. Therefore, this study, we aimed develop microfluidic method unroof cells observe intrinsic molecules organelles. In our method, single were precisely arrayed on bottom surface microchannels light-guided manner...

A microfluidic cell stretch device was developed to investigate the effects of stretching stress on pulmonary artery smooth muscle (PASMC) proliferation in arterial hypertension (PAH). The harbors upper culture and lower control channels, separated by a stretchable poly(dimethylsiloxane) membrane that acts as substrate. channel inlet connected vacuum pump via digital switch-controlled solenoid valve. For cyclic at heartbeat frequency (80 bpm), open or close time for each valve set 0.38 s....

A 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer hydrogel exhibits a remarkable ability to preserve cells on microfluidic chips without perfusion culture under cell-based assay conditions, with characteristics of maintenance high cell viability, restraint proliferation, and minimization cellular function loss over period days weeks. This would establish revolutionary flexibility for applications. Detailed facts importance specialist readers are published as "Supporting Information"....

A palmtop‐sized microfluidic cell culture system is presented. The consists of a device and miniaturized infusion pump that possesses reservoir medium, an electrical control circuit, internal battery. footprint the was downsized to 87 × 57 mm, which is, best our knowledge, smallest integrated system. Immortalized human microvascular endothelial cells ( HMEC ‐1) umbilical vein HUVEC ) were cultured in ‐1 proliferated at same speed as microchannel perfused by syringe flask. oriented along...