Using an SDS gel electrophoresis method, connectin, very high molecular weight ( ˜106 dalton) protein, was detected in extract of whole tissues various types muscles vertebrates and invertebrates. Connectin bands were clearly recognized all the striated (skeletal cardiac) examined: rabbit, chicken, turtle, snake, newt, frog, fish. This also case with skeletal muscle prochordate, Amphioxus. In invertebrates, situation much complicated. Connectin-like protein C. elegans (nematode), but not...

Herpes simplex virus type 1 (HSV-1) is a highly prevalent in humans and causes severe forms of inflammation, such as herpes encephalitis (HSE). Pyroptosis new inflammatory cell death triggered by inflammasome cysteine-requiring aspartate protease-1 (caspase-1) activation. Nonetheless, HSV-1 induces encephalitis, mechanisms are not understood. In this study, we confirmed for the first time that DNA triggers Gasdermin D-dependent pyroptosis activating NLR family pyrin domain containing 3...

Purpose To determine the measurement reliability of CorVis ST, a dynamic Scheimpflug analyser, in virgin and post-photorefractive keratectomy (PRK) eyes compare results between these two groups. Methods Forty 42 post-PRK underwent ST measurements performed by technicians. Repeatability was evaluated comparing three consecutive technician A. Reproducibility determined first A with one B. Intraobserver interobserver intraclass correlation coefficients (ICCs) were calculated. Univariate...

Background: Glaucoma is a leading cause of irreversible blindness. Remodeling the scleral extracellular matrix (ECM) plays an important role in development glaucoma. The aim this study was to identify key genes and pathways for ECM remodeling sclera glaucoma by bioinformatics analysis explore potential therapeutic agents management. Methods: Genes associated with glaucoma, were detected using text mining tool pubmed2ensembl, assigned Gene Ontology (GO) biological process terms Kyoto...

Scleral extracellular matrix (ECM) remodeling is thought to play a critical role in the pathogenesis of glaucoma. Mechanical strain induced by elevated intraocular pressure can promote myofibroblast differentiation fibroblasts and result scleral ECM remodeling; however, underlying mechanism remains poorly understood. Yes-associated protein (YAP) mechanosensory key downstream transcriptional effector Hippo signaling pathway. Here, we investigated YAP mechanical strain-induced transformation...

Bladder cancer (BC) is the most common malignancy of urinary system. Pyroptosis a host programmed cell death. However, effects pyroptosis-related lncRNAs (PRLs) on BC have not yet been completely elucidated. In this study, prognostic PRLs model and two ceRNA networks were established using sufficient bioinformatics analysis preliminary RT-qPCR validation in vitro . 6 identified to construct model. Then, risk score was verified be an effective independent factor (Training cohort: Univariate...

Alzheimer's disease (AD) is a neurodegenerative condition causing cognitive decline. Oxidative stress (OS) believed to contribute neuronal death and dysfunction in AD. We conducted study identify differentially expressed OS-related genes (DEOSGs) through bioinformatics analysis experimental validation, aiming develop diagnostic model for analyzed the GSE33000 dataset OS regulator expression profiles create molecular clusters (C1 C2) associated with immune cell infiltration using 310 AD...

To investigate potential diagnostic and prognostic biomarkers associated with prostate cancer (PCa), we obtained gene expression data from six datasets in the Gene Expression Omnibus (GEO) database. The included 127 PCa cases 52 normal controls. We filtered for differentially expressed genes (DEGs) identified candidate using a least absolute shrinkage selector operation (LASSO) regression model support vector machine recursive feature elimination (SVM-RFE) analyses. A difference analysis was...

The binding of actin filaments to connectin, a muscle elastic protein, was investigated by means turbidity and sedimentation measurements electron microscopy. In the presence less than 0.12 M KCI at pH 7.0, bound connection. Long formed bundles. Short also aggregated into irregular bundles or meshwork, were frequently attached perpendicularly long F-actin connectin saturated an equal weight ratio (molar ratio, 50 : 1) as determined cosedimentation assay. Larger amounts sonicated short...

In October 16th issue of Nature (1986), Louis Kunkel and his associates Harvard Medical School announced the isolation gene defect which results in Duchenne muscular dystrophy showed that its mRNA is as large 16 kb.l)This suggests product has a huge molecular weight more than 500,000.The possible candidate proteins, hitherto known, are nebulin (MW, 700 kDa) connectin (also called titin; MW, 3000 skeletal muscle, first suggested by Orkin.2>Nebulin was isolated denatured form localization at...

Currently, Alzheimer’s disease (AD) and type 2 diabetes mellitus (T2DM) are widely prevalent in the elderly population, accumulating evidence implies a strong link between them. For example, patients with T2DM have higher risk of developing neurocognitive disorders, including AD, but exact mechanisms still unclear. This time, by combining bioinformatics analysis vivo experimental validation, we attempted to find common biological AD T2DM. We firstly downloaded gene expression profiling (AD:...

By SDS-gel electrophoresis using 1.8% polyacrylamide gel, and 1.5% plus 0.3% agarose myosin heavy chain oligomers as molecular mass standard, the of nebulin was determined to be approximately 800 kDa.This value in agreement with that estimated from size mRNA.

To evaluate the anterior segment biometry of Tetraflex accommodating intraocular lens (AIOL) and contribution forward movement to amplitude accommodation (AMP).Patients who underwent phacoemulsification with implantation AIOLs control nonaccommodating lenses were imaged by custom-built, long scan depth spectral-domain optical coherence tomography at relaxed maximal accommodative states. Anterior was performed correlated clinical manifestation including AMP.Patients in group showed better...

This study aimed to investigate the common molecular mechanism between obesity and papillary thyroid cancer (PTC), most pathological type of cancer. In this study, we obtained gene expression datasets for (GSE151839) PTC (GSE33630) from Gene Expression Omnibus (GEO). We used Perl program R software identify differentially expressed genes (DEGs) genes, perform GO function KEGG pathway enrichment analysis, construct a protein-protein interaction (PPI) network, hub transcription factors (TFs)...

Abstract Background Colon cancer (CC) is a prevalent malignant tumor that affects the colon in gastrointestinal tract. Its aggressive nature, strong invasiveness, and rapid progression make it significant health concern. In addition, oxidative stress can lead to production of reactive oxygen species (ROS) surpass body's antioxidant defense capacity, causing damage proteins, lipids, DNA, potentially promoting development. However, relationship between CC requires further investigation....

Purpose. To identify the potential key genes and molecular pathways associated with keratoconus allergic disease. Methods. The pubmed2ensembl database was used to text mining (TMGs) collectively involved in GeneCodis program perform Gene Ontology (GO) biological process Kyoto Encyclopedia of Genes Genomes (KEGG) pathway enrichment analysis TMGs. protein-protein interaction (PPI) network TMGs established by STRING; significant gene modules hub PPI were further performed using Cytoscape...

Phenylalanine ammonia-lyase (PAL) is the key entry enzyme of plant phenylpropanoid pathway. It plays an important role in biosynthesis podophyllotoxin, anti-tumor lignan that currently produced from its main natural source Sinopodophyllum hexandrum (Royle) Ying. In this study, we cloned gene ShPAL encoding phenylalanine by RT-PCR root S. ecotype inhabited Aba' district, Sichuan, based on public SRA transcriptome data-package. Bioinformatics analyses showed ShPAL-encoded protein composed 711...