A5080812486- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Biochemical Analysis and Sensing Techniques
- Neuroendocrine regulation and behavior
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Neuropeptides and Animal Physiology
- Retinal Development and Disorders
- Molecular Sensors and Ion Detection
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Nicotinic Acetylcholine Receptors Study
- Biochemical effects in animals
- Free Radicals and Antioxidants
- Anesthesia and Sedative Agents
- Synthesis and Reactions of Organic Compounds
- Axial and Atropisomeric Chirality Synthesis
- Cancer-related Molecular Pathways
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Connexins and lens biology
- Ion Channels and Receptors
- Molecular spectroscopy and chirality
- Nuclear Receptors and Signaling
- Antioxidant Activity and Oxidative Stress
Institute of Evolutionary Physiology and Biochemistry
2009-2022
Russian Academy of Sciences
2008-2014
Tver State University
2009
A. N. Nesmeyanov Institute of Organoelement Compounds
1985
It has recently been proposed that extracellular signal-regulated kinases 1 and 2 (ERK1/2) are one of the factors mediating seizure development. We hypothesized inhibition ERK1/2 activity could prevent audiogenic seizures by altering GABA glutamate release mechanisms. Krushinsky-Molodkina rats, genetically prone to seizure, were recruited in experiments. Animals i.p. injected with an inhibitor SL 327 at different doses 60 min before audio stimulation. demonstrated for first time injections...
Non‐technical summary Calcium‐permeable (CP) AMPA receptors play an important role in many synaptic functions and neuronal death. In this study, we investigated the blocking activity of a selective CP‐AMPAR channel blocker, IEM‐1925, discovered that blocker preferentially inhibits receptor at increased frequency synapse stimulation continuous presence agonist. The activity‐dependent block described study may serve as useful means for answering questions about specific CP‐AMPARs, it opens new...
Dehydration activates the vasopressinergic system of hypothalamus. We analyzed effects dehydration induced by water deprivation for 3 days on activities ERK1/2 and transcription factors, Elk1 cAMP response element-binding protein (CREB) in neurons, as well distribution level motor protein, kinesin, hypothalamo-neurohypophyseal system. showed that resulted enhanced vasopressin (VP) expression activation CREB, increased activity MEK/ERK/Elk1 pathway magnocellular neurons supraoptic nucleus....
Recently, we demonstrated that inhibition of ERK1/2 activity by SL-327 treatment blocks seizure behavior in Krushinsky-Molodkina (KM) rats, which was mediated altering GABA and glutamate release mechanism the hippocampus. Basal ganglia representing various subcortical cell groups play a significant role regulation motor activity, including epileptiform seizures.Objectives: To verify if nigrostriatal system could be also affected analyzed expression tyrosine hydroxylase, D1 D2 dopamine...
It is known that perirhinal/insular cortices participate in the transmission of sensory stimuli to motor cortex, thus coordinating activity during seizures. In present study we analysed seizure-related proteins, such as GABA, glutamate, ERK1/2 and synaptic proteins insular cortex Krushinsky-Molodkina (KM) rats genetically prone audiogenic seizures (AGS). We compared seizure-naïve seizure-experienced KM with control Wistar order distinguish whether protein changes are associated seizure event...
The effects of divalent cations on Ca2+-impermeable containing (GluR2 subunit) MPA receptors hippocampal pyramidal neurones isolated from rat brain was studied using patch-clamping. Ca2+, Mg2+, Mn2+, Co2+, Ni2+ and Zn2+ inhibited currents induced by kainate glutamate. Inhibition fast, reversible voltage independent. rank order activities > Co2+ Ca2+ Mn2+ Mg2+. Cyclothiazide (0.1 mm) significantly reduced inhibition 6, 7 dinitroquinoxaline-2.3-dione (DNQX). However, high concentrations DNQX...
To reveal abnormalities in the inferior colliculus during postnatal development of seizure susceptibility audiogenic rats, an analysis neurochemical markers, which characterized a functional state glutamate and GABA neurons, was done. Krushinsky-Molodkina rats (KM) were used experiments. These demonstrate age-dependent seizures (AGS), expression is regulated by brain stem structures such as colliculus. In critical period AGS (P14, P30, P60) KM activity ERK1/2 kinase significantly increased....
Neurochemical mechanisms of initiation and expression epileptic seizures are poorly explored, there no published data that could demonstrate the functional state neuromediator systems at initial seizure in animals genetically prone to seizure. In current work, we studied role extracellular signal-regulated kinase (ERK1/2) regulation nigrostriatal glutamate, GABA dopamine neurons Krushinsky – Molodkina rats clonus-tonus ataxia stages audiogenic We demonstrated upregulation ERK1/2 activity...