A5048468302- Corneal Surgery and Treatments
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Iron oxide chemistry and applications
- Periodontal Regeneration and Treatments
- Intraocular Surgery and Lenses
- Corneal surgery and disorders
- Nanofabrication and Lithography Techniques
- Surgical Sutures and Adhesives
- Advancements in Photolithography Techniques
- Magnesium Alloys: Properties and Applications
- Mesenchymal stem cell research
- Intermetallics and Advanced Alloy Properties
- Medical Research and Treatments
- Energetic Materials and Combustion
- Wound Healing and Treatments
- Plant Virus Research Studies
- RNA Interference and Gene Delivery
- Ocular Surface and Contact Lens
- Metal and Thin Film Mechanics
- Mycobacterium research and diagnosis
- Adhesion, Friction, and Surface Interactions
Shandong Agricultural University
2023-2024
St. Jude Children's Research Hospital
2020-2021
University of Tennessee Health Science Center
2021
Huazhong University of Science and Technology
2019-2020
Tongji Hospital
2019-2020
Hainan University
2014
Purpose: To compare the difference in gene expression between human limbal niche cells (LNC) and bone marrow derived mesenchymal stem (BMMSC).Methods: LNC were isolated by collagenase expanded modified embryonic cell medium (MESCM) on a Matrigel coated plastic plate.Cell diameters measured with Image J software.Relative levels BMMSC compared using Affymetrix Human Primer View Gene Expression Array.A subset of differentially expressed genes was verified RT-qPCR.The protein level LAMA1 COL4A1...
The aim was to reveal the characteristic profiles of marketed levofloxacin eye drops (5 mg/ml) and gel (3 mg/g) from pharmacokinetics pharmacodynamics views rabbits' eyes. A mild a heavy bacterial keratitis models in rabbits were established. Different regimens gel, including phosphate buffer solution (the PBS group), 4-Sol + 1-Gel group (rabbits treated with 4 doses 1 dose per day), 3-Sol gel), (4 drops), 4-Gel 3-Gel applied evaluate their efficacies. ocular also investigated. results...
T reg cells bearing a diverse antigen receptor repertoire suppress pathogenic and maintain immune homeostasis during their long lifespan. How robust function is determined genetically remains elusive. Here, we investigate the regulatory space of cis-regulatory elements lineage–specifying factor Foxp3. Foxp3 enhancers are known as distinct readers environmental cues controlling cell induction or lineage stability. However, single deficiencies cause mild, if any, dysregulation, leaving key...
Abstract Restricted by the difficulty in fabricating scaffolds suitable for cell proliferation, use of ex vivo expanded limbal stem (LSC) LSC transplantation, an effective treatment method patients with limb deficiency (LSCD), is hard to be widely used clinical practice. To tackle these challenges, a novel electrospun polycaprolactone (PCL)/gelatin nanocomposite proposed make 3D niche cells (LNC) proliferation vitro, which milestone diseases such as LSCD. PCL and gelatin different weight...
Abstract BACKGROUND Porcine infection with circovirus type 2 (PCV2) causes immunosuppression, which is easy to cause concurrent or secondary infection, making the disease complicated and difficult treat, causing huge economic losses pig industry. Total polysaccharide from rhizoma of Atractylodes macrocephala Koidz. (PAMK) outstanding in enhancing non‐specific immunity cellular immunity, effectively improving body's resistance, indicating its potential role antiviral immunotherapy. RESULTS...
ABSTRACT Purpose To investigate the possibility and key factors of stably committed mature corneal epithelial cells dedifferentiate into stem in vitro . Methods Mature cornea epithelia cell (MCEC) sheets or limbal progenitor (LEPC) were isolated from central corneas segments by Dispase II further digested with 0.25% trypsin/1 mM EDTA (T/E) to yield single cells. Limbal niche (LNC) stroma collagenase A expanded on 5% Matrigel coated plastic. Single MCECs seeded 50% without LNC culturing for...
Nuclear factor Foxp3 sustains immune-suppressive function of regulatory T (Treg) cells during their long lifespan, raising a fundamental question about how stochastic induction is transformed into stable expression Treg lineage commitment. To address this issue, we examined the processes development and observed an abrupt switch transcriptional mechanisms regulating expression. By integrating genetic pharmacological approaches with unbiased CRISPR screening, discovered several known novel...