The effect of intravenous administration human mesenchymal stromal stem cells (hMSC) has been evaluated by means large-conductance calcium-dependent potassium channel (BKCa) activity measurements in thoracic aorta smooth muscle (SMC) obtained from non-fatal whole-body irradiated rats, using the patch clamp technique whole-cell modification, and standard acetylcholine (ACh) test to evaluate functional endothelium integrity SM contractile recordings. Myofilament calcium sensitivity was...

Hydrogen sulfide (H2S) is considered as a protective factor against cardiovascular disorders. However, there are few reports on the effects of H2S in central nervous system during stress or injury. Previous studies goldfish have shown that astrocytic response occurs damaged and contralateral optic nerves. Glial fibrillary acidic protein (GFAP) concentration nerves rainbow trout has not been measured previously. This study further characterized nerve brain (Oncorhynchus mykiss) after...

A study of the lateral pallium in zebrafish and visual tectum medaka revealed a population adult neuroepithelial (NE) cells supported from early stage development to various postembryonic stages ontogenesis. These data emphasize importance non-radial glial stem neurogenesis animals, particular fish. However, distribution, cell cycle features, molecular markers NE progenitors fish are still poorly understood at Fetalization predominates ontogenetic salmon fish, which is associated with delay...

The numerous caspase-like activities present in nervous tissue can be investigated with labelled peptides. However, the cross-reactivities of peptides both proteasomes and caspases complicate analysis protease activity. pharmacological features substrates inhibitors specific for either or proteasome proteases rat brain lysates were similar identical to profiles commercially purified preparations. Caspase bind directly active centres, thus competing selective antagonists proteasomes....

Existing data suggest the involvement of cytoskeletal proteins, particularly actin, in process apoptosis. However, exact role actin remains unclear. The aim this study was to investigate potential functional interaction protein with one key participants apoptosis, cytochrome c. We utilized fluorescent labeling amino acid residues rat brain-derived b/g-actin its monomeric form (G-actin) using dye 3-(4-carboxybenzoyl) quinoline2-carboxaldehyde (CBQCA) monitor induced molecular changes. It is...

Позаклітинні везикули мають дуже багато фізіологічних функцій: від регуляції експресії генів, до прямих цитотоксичних ефектів.Існує селективний механізм, який об'єднує формування нових везикул, їх рецепцію і складну переробку вмісту

Little-known to a wide range of specialists details the functioning one main participants in cellular metabolism – complex neutral proteases with their regulators, which is called “proteasome” are observed this paper. The review analyzes works recent years devoted study participation proteasomes intercellular signaling and catabolism regulatory proteins extracellular space.

The activities of two classes neutral proteases were studied: caspases and proteasomes, which could be contained in vesicles generated by human stem cells. formation apoptosomes not induced mesenchymal hematopoietic cells (hereinafter—MSCs HSCs, respectively) with the participation cytochrome C. Caspase activity was found culture medium exosomes after tumor necrosis factor α (TNFα) supplementation only. This is completely inhibited a non-substrate caspase inhibitor emricane, sensitive to...

Activities of two classes neutral proteases were studied: caspases and proteasomes, which are contained in extracellular vesicles generated by mesenchymal hematopoietic human stem cells (MSCs HSCs, respectively). The formation apoptosomes induced the cytochrome C dATP did not occurred these cells. presence TNFa culture medium HSCs causes appearance caspase-3 intracellular activity. Herewith caspase activity was detected exosomes also. This completely inhibited a non-substrate inhibitor,...

We investigated 2′-deoxyadenosine 5′-triphosphate (dATP) and cytochrome C-induced apoptosome formation as a source of reliable natural process programmed cell death in the brain newborn rats. tried to find out which probable participants apoptosis is responsible for nonlinear growth at moment initiation their assembly. It was found that stimulation actin assembly by various substances, example, Ribonuclease A (RNase A) or cations (Na+ K+), leads induction formation. Actin polymerization...

Necessary and sufficient conditions are given for a positive homogeneous function of three variables to be representable as difference convex functions.

The goal of the paper is development an optimization method with superlinear convergence rate for a nonsmooth convex function. For approximation used that similar to Steklov integral averaging. difference averaging performed over variable-dependent set, called set-valued mapping (SVM) satisfying simple conditions. Novelty approach such we obtain twice continuously differentiable functions, optimizations which are applied methods second order. estimation given.