In adult fish, neurogenesis occurs in many areas of the brain, including cerebellum, with ratio newly formed cells relative to total number brain being several orders magnitude greater than mammals. Our study aimed compare expressions aromatase B (AroB), glutamine synthetase (GS), and cystathionine-beta-synthase (CBS) cerebellum intact juvenile chum salmon, Oncorhynchus keta. To identify dynamics that determine involvement AroB, GS, CBS cellular mechanisms regeneration, we performed a...

The ultrastructural organization of the nuclei tegmental region in juvenile chum salmon (Oncorhynchus keta) was examined using transmission electron microscopy (TEM). dorsal (DTN), nucleus fasciculus longitudinalis medialis (NFLM), and oculomotor nerve (NIII) were studied. examination provided detailed characteristics neurons forming showed neuro-glial relationships them. Neurons three size types with a high metabolic rate, characterized by presence numerous mitochondria, polyribosomes,...

Studying the properties of neural stem progenitor cells (NSPCs) in a fish model will provide new information about organization neurogenic niches containing embryonic and adult cells, reflecting their development, origin cell lines proliferative dynamics. Currently, molecular signatures these populations homeostasis repair vertebrate forebrain are being intensively studied. Outside telencephalon, regenerative plasticity NSPCs biological significance have not yet been practically The...

We investigated the distribution of gamma aminobutyric acid, tyrosine hydroxylase and nitric oxide-producing elements in a cherry salmon Oncorhynchus masou brain at various stages postnatal ontogenesis by immunohistochemical staining histochemical staining. The periventricular region cells exhibited morphology neurons glia including radial glia-like contained several neurochemical substances. Heterogeneous populations hydroxylase-, acid-immunoreactive, as well nicotinamide adenine...

Fish are a convenient model for the study of reparative and post-traumatic processes central nervous system (CNS) recovery, because formation new cells in their CNS continues throughout life. After traumatic injury to cerebellum juvenile masu salmon, Oncorhynchus masou, cell composition neurogenic zones containing neural stem (NSCs)/neural progenitor (NPCs) acute period (two days post-injury) changes. The presence neuroepithelial (NE) radial glial (RG) neuronal precursors located dorsal,...

The central nervous system of Pacific salmon retains signs embryonic structure throughout life and a large number neuroepithelial neural stem cells (NSCs) in the proliferative areas brain, particular. However, adult neurogenesis studies on rainbow trout, Oncorhynchus mykiss, are limited. Here, we studied localization glutamine synthetase (GS), vimentin (Vim), nestin (Nes), as well neurons formed postembryonic period, labeled with doublecortin (DC), under conditions homeostatic growth...

The considerable post-traumatic brain recovery in fishes makes them a useful model for studying the mechanisms that provide reparative neurogenesis, which is poorly represented mammals. After mechanical injury to telencephalon adult fish, lost neurons are actively replaced due proliferative activity of neuroepithelial cells and radial glia neurogenic periventricular zone. However, it not enough clear signaling involved activation neural stem (aNSC) after (reactive proliferation) production...

Fishes have remarkable ability to effectively rebuild the structure of nerve cells and fibers after central nervous system injury. However, underlying mechanism is poorly understood. In order address this issue, we investigated proliferation apoptosis in contralateral ipsilateral optic nerves, stab wound injury eye an adult trout Oncorhynchus mykiss. Heterogenous population proliferating was at 1 week TUNEL labeling gave a qualitative quantitative assessment 2 days After injury, apoptotic...

Hydrogen sulfide (H2S) is considered as a protective factor against cardiovascular disorders. However, there are few reports on the effects of H2S in central nervous system during stress or injury. Previous studies goldfish have shown that astrocytic response occurs damaged and contralateral optic nerves. Glial fibrillary acidic protein (GFAP) concentration nerves rainbow trout has not been measured previously. This study further characterized nerve brain (Oncorhynchus mykiss) after...

The proliferation of neural stem cells (NSCs)/neuronal precursor (NPCs) and the occurrence postmitotic neuroblasts in mesencephalic tegmentum intact juvenile chum salmon, Oncorhynchus keta, at 3 days after a tegmental injury, were studied by immunohistochemical labeling. BrdU+ constitutive progenitor located both periventricular matrix zone deeper subventricular parenchymal layers brain are revealed salmon. As result traumatic damage to tegmentum, resident neuroepithelial type increases....

In contrast to astrocytes in mammals, fish promote axon regeneration after brain injury and actively participate the process. Neurolin, a regeneration-associated, Zn8-labeled protein, is involved repair of damaged optic nerve goldfish. At 1 week unilateral eye injury, expression neurolin chiasm, Pax6 that influences nervous system development various regions rainbow trout (Oncorhynchus mykiss) were detected. Immunohistochemical staining revealed number Zn8+ cells head intraorbital segment...

A study of the lateral pallium in zebrafish and visual tectum medaka revealed a population adult neuroepithelial (NE) cells supported from early stage development to various postembryonic stages ontogenesis. These data emphasize importance non-radial glial stem neurogenesis animals, particular fish. However, distribution, cell cycle features, molecular markers NE progenitors fish are still poorly understood at Fetalization predominates ontogenetic salmon fish, which is associated with delay...

In the brain of teleost fish, radial glial cells are major type astroglial cells. To answer question as to how glia structures adapt continuous growth brain, which is characteristic salmonids, it necessary study various types (neuronal precursors, cells, and in a state neuronal differentiation) integrative centers salmon (telencephalon tectum opticum), using rainbow trout, Oncorhynchus mykiss, model. A distribution several molecular markers telencephalon with identification neural...

The distribution of proliferative zones, NO-producing cells and apoptosis areas in the medulla oblongata, cerebellum, optic tectum, thalamus hypothalamus Amur sturgeon Acipenser schrenckii was investigated, using techniques immunoperoxidase staining proliferating cell nuclear antigen (PCNA), neuronal nitric oxide synthase TU-NEL-labeling fragmented DNA. It has been established, that brain NO can act both as a cytotoxic proapoptogenic factor, which stimulates proliferation. presence elements...