A5038498078- Wastewater Treatment and Nitrogen Removal
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Genomics and Phylogenetic Studies
- Chromium effects and bioremediation
- Anaerobic Digestion and Biogas Production
- Phosphorus and nutrient management
- Constructed Wetlands for Wastewater Treatment
- Bacteriophages and microbial interactions
- Water Quality Monitoring Technologies
- Advanced Technologies in Various Fields
- Gut microbiota and health
- Energy Harvesting in Wireless Networks
- Methane Hydrates and Related Phenomena
- Environmental DNA in Biodiversity Studies
- Historical and Archaeological Studies
- Ammonia Synthesis and Nitrogen Reduction
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Marine and environmental studies
- Botany and Plant Ecology Studies
- Bacterial biofilms and quorum sensing
- Oral and gingival health research
- Protist diversity and phylogeny
- Lichen and fungal ecology
- Opportunistic and Delay-Tolerant Networks
Aalborg University
2018-2025
Ecologie Microbienne Lyon
2018
Abstract Microorganisms play crucial roles in water recycling, pollution removal and resource recovery the wastewater industry. The structure of these microbial communities is increasingly understood based on 16S rRNA amplicon sequencing data. However, such data cannot be linked to functional potential absence high-quality metagenome-assembled genomes (MAGs) for nearly all species. Here, we use long-read short-read recover 1083 MAGs, including 57 closed circular genomes, from 23 Danish...
Abstract Enhanced biological phosphorus removal (EBPR) is a globally important biotechnological process and relies on the massive accumulation of phosphate within special microorganisms. Candidatus Accumulibacter conform to classical physiology model for polyphosphate accumulating organisms are widely believed be most player in full-scale EBPR systems. However, it was impossible till now quantify contribution specific microbial clades EBPR. In this study, we have developed new tool directly...
Abstract Members of the genus Dechloromonas are often abundant in enhanced biological phosphorus removal (EBPR) systems and recognized putative polyphosphate accumulating organisms (PAOs), but their role phosphate is still unclear. Here, we used 16S rRNA gene sequencing fluorescence situ hybridization (FISH) to investigate abundance distribution spp. Danish global wastewater treatment plants. The two most species worldwide revealed dynamics important intracellular storage polymers, measured...
The bacterial genus Tetrasphaera encompasses abundant polyphosphate accumulating organisms (PAOs) that are responsible for enhanced biological phosphorus removal (EBPR) in wastewater treatment plants. Recent analyses of genomes from pure cultures revealed 16S rRNA genes cannot resolve the lineage, and spp. several different genera within Dermatophilaceae. Here, we examine 14 recently recovered high-quality metagenome-assembled plants containing full-length identified as Tetrasphaera, 11...
The Saprospiraceae family within the phylum Bacteroidota is commonly present and highly abundant in wastewater treatment plants (WWTPs) worldwide, but little known about its role. In this study, we used MiDAS 4 global survey with samples from 30 countries to analyze abundance distribution of members Saprospiraceae. Phylogenomics were delineate five new genera a set 31 high-quality metagenome-assembled genomes Danish WWTPs. Newly designed probes for fluorescence situ hybridization (FISH)...
Filamentous bacteria belonging to the phylum Chloroflexi have received considerable attention in wastewater treatment systems for their suggested role operational problem of impaired sludge settleability known as bulking. Their consistently high abundance full-scale systems, even absence bulking, indicates that they make a substantial contribution nutrient transformations during treatment. In this study, extensive 16S rRNA amplicon surveys Danish plants (WWTPs) with removal were screened...
Microbial communities in water resource recovery facilities encompass a large diversity of poorly characterized lineages that could have undescribed process-critical functions. Recently, it was shown taxa belonging to "Acidobacteriota" are abundant Danish full-scale activated sludge wastewater treatment plants (WWTP), and here we investigated their diversity, distribution, functional potential. were identified using comprehensive full-length 16S rRNA gene reference dataset amplicon...
"Candidatus Accumulibacter" was the first microorganism identified as a polyphosphate-accumulating organism (PAO) important for phosphorus removal from wastewater. Members of this genus are diverse, and current phylogeny taxonomic framework appear complicated, with most publicly available genomes classified Accumulibacter phosphatis," despite notable phylogenetic divergence. The ppk1 marker gene allows finer-scale differentiation into different "types" "clades"; nevertheless, assignments...
Abstract Cable bacteria of the Desulfobulbaceae family are centimeter-long filamentous bacteria, which capable conducting long-distance electron transfer. Currently, all cable classified into two candidate genera: Candidatus Electronema, typically found in freshwater environments, and Electrothrix, saltwater environments. This taxonomic framework is based on both 16S rRNA gene sequences metagenome-assembled genome (MAG) phylogenies. However, most currently available MAGs highly fragmented,...
Phosphorus (P) is present in activated sludge from wastewater treatment plants the form of metal salt precipitates, extracellular polymeric substances, or bound into biomass, for example, as intracellular polyphosphate (poly-P). Several methods a reliable quantification different P-fractions have recently been developed, and this study combines them to obtain comprehensive P mass-balance four enhanced biological phosphate removal (EBPR) plants. Chemical characterization by ICP-OES sequential...
Anaerobic digestion is a key process for the conversion of waste organics to biogas energy and reliant on synergistic activities complex microbial communities. Members phylum Chloroflexi are often found be abundant in these systems, yet little known their role, with most members cultured or identified. The aim this study was characterize communities present full-scale anaerobic digesters receiving excess sludge from wastewater treatment plants. core genus-level-phylotypes were identified...
Microthrix is one of the most common bulking filamentous microorganisms found in activated sludge wastewater treatment plants (WWTPs) across globe. One species,
Chloroflexota are often abundant members of the biomass in wastewater treatment plants (WWTPs) worldwide, typically with a filamentous morphology, forming backbones activated sludge floc. However, their overgrowth can cause operational issues connected to poor settling or foaming, impairing effluent quality and increasing costs. Despite importance, few genera have been characterized so far. Here, we present comprehensive overview WWTPs worldwide an in-depth characterization phylogeny,...
ABSTRACT Species belonging to the genus Leptothrix are widely distributed in environment and activated sludge (AS) wastewater treatment plants (WWTPs). They commonly found iron-rich environments reported cause filamentous bulking WWTPs. In this study, diversity, distribution, metabolic potential of most prevalent spp. AS worldwide were studied. Our 16S rRNA amplicon survey showed that belongs general core community worldwide, comprising 32 species with four being found. Their taxonomic...
Abstract Microorganisms are critical to water recycling, pollution removal and resource recovery processes in the wastewater industry. While structure of this complex community is increasingly understood based on 16S rRNA gene studies, cannot currently be linked functional potential due absence high-quality metagenome-assembled genomes (MAGs) with full-length genes for nearly all species. Here, we sequence 23 Danish full-scale treatment plant metagenomes, producing >1 Tbp long-read...
Abstract Members of the genus Dechloromonas are often abundant in enhanced biological phosphorus removal (EBPR) systems and recognized putative polyphosphate accumulating organisms (PAOs), but their role phosphate (P) is still unclear. Here, we used 16S rRNA gene sequencing fluorescence situ hybridization (FISH) to investigate abundance distribution spp. Danish wastewater treatment plants. Two species were abundant, novel, uncultured, could be targeted by existing FISH probes. Raman...
Summary The last 20 years have witnessed unprecedented advances in revealing the microbiomes underpinning important processes natural and human associated environments. Recent large-scale metagenome surveys record variety of microbial life oceans 1 , wastewater 2 gut 3,4 earth 5,6 with compilations encompassing thousands public datasets 7–13 . So far, microbiome studies either miss functional information or consistency sample processing, although they may cover locations, these are missing...
Abstract Large amounts of phosphorus (P) are present in activated sludge from municipal wastewater treatment plants, where it exists the form metal salt precipitates or biologically bound into biomass as nucleic acids, cell membrane components, and extracellular polymeric substances or, special polyphosphate-accumulating organisms (PAOs), intracellular polyphosphate. Only recently, methods that reliably allow an absolute quantification different P-fractions, such sequential extraction, Raman...
Abstract Filamentous bacteria belonging to the phylum Chloroflexi have received considerable attention in wastewater treatment systems for their suggested role operational problem of impaired sludge settleability known as bulking. Their consistently high abundance full-scale systems, even absence bulking, indicates that they make a substantial contribution nutrient transformations during treatment. In this study, extensive 16S rRNA amplicon surveys Danish WWTPs were screened identify most...
Abstract Filamentous Chloroflexota are abundant in activated sludge wastewater treatment plants (WWTPs) worldwide and occasionally associated with poor solid-liquid separation or foaming, but most of the lineages remain undescribed. Here, we present a comprehensive overview WWTPs worldwide, using high-quality metagenome-assembled genomes (MAGs) 16S rRNA amplicon data from 740 Danish global WWTPs. Many novel taxa were described, encompassing 4 families, 13 genera 29 species. These widely...
Abstract Cable bacteria of the Desulfobulbaceae family are centimeter-long filamentous bacteria, which capable conducting long-distance electron transfer. Currently, all cable classified into two candidate genera: Candidatus Electronema, typically found in freshwater environments, and Electrothrix, saltwater environments. This taxonomic framework is based on both 16S rRNA gene sequences metagenome-assembled genome (MAG) phylogenies. However, most currently available MAGs highly fragmented,...
Abstract Genomes are fundamental to understanding microbial ecology and evolution. The emergence of high-throughput, long-read DNA sequencing has enabled recovery genomes from environmental samples at scale. However, expanding the genome catalogue soils sediments been challenging due enormous complexity these environments. Here, we performed deep, Nanopore 154 soil sediment collected across Denmark through an optimised bioinformatics pipeline, recovered 15,314 novel species, including 4,757...