A5002932434- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- HIV/AIDS drug development and treatment
- Respiratory viral infections research
- Pharmacological Effects of Natural Compounds
- Bacteriophages and microbial interactions
- Immunotherapy and Immune Responses
- Biochemical and Molecular Research
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- SARS-CoV-2 detection and testing
- Synthesis and biological activity
- Cytomegalovirus and herpesvirus research
- Viral gastroenteritis research and epidemiology
- Drug-Induced Hepatotoxicity and Protection
- RNA Research and Splicing
- Immune Response and Inflammation
- Viral Infectious Diseases and Gene Expression in Insects
- interferon and immune responses
- Biosensors and Analytical Detection
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA modifications and cancer
State Research Center of Virology and Biotechnology VECTOR
2019-2025
Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2021-2025
One of the key stages in development mRNA vaccines is their delivery. Along with liposome, other materials are being developed for delivery that can ensure both safety and effectiveness vaccine, also facilitate its storage transportation. In this study, we investigated polyglucin:spermidine conjugate as a carrier an mRNA-RBD vaccine encoding receptor binding domain (RBD) SARS-CoV-2 spike protein. The conditions self-assembling mRNA-PGS complexes were optimized, including selection mRNA:PGS...
In this study, we characterized recombinant hemagglutinin (HA) of influenza A (H5N8) virus produced in Chinese hamster ovary cells (CHO-K1s). Immunochemical analysis showed that the was recognized by serum ferrets infected with virus, indicating its antigenic properties were retained. Two groups Balb/c mice immunized intramuscular injection or propiolactone inactivated A/Astrakhan/3212/2020 virus. The results demonstrated both immunogens induced a specific antibody response as determined...
Highly pathogenic avian influenza (HPAI) H5 clade 2.3.4.4b viruses are widespread in wild and domestic birds, causing severe economic damage to the global poultry industry. Moreover, of this known cause infections mammals, posing a potential pandemic threat. Due ongoing evolution change dominant strains 2.3.4.4b, it is important investigate cross-reactivity vaccines use under development against viruses. In study, immunogenicity previously developed DNA vaccine encoding modified...
Avian influenza A virus subtype H5 is widespread in the bird population, and there a risk of its transmission to humans, therefore, it necessary develop an effective vaccine prevent spread. The aim work was prototypes DNA vaccines encoding modified variants hemagglutinin trimer highly pathogenic avian (H5N8) fragment (stem) study their immunogenic properties. Material methods. constructs were designed, produced purified, expression analyzed eukaryotic cells using reverse transcription PCR...
Background: Although mRNA vaccines encapsulated in lipid nanoparticles (LNPs) have demonstrated a safety profile with minimal serious adverse events clinical trials, there is opportunity to further reduce reactogenicity. The development of naked could improve vaccine tolerability. Naked nucleic acid delivery using the jet injection method may be solution. Methods: In first part study, optimal conditions providing low traumatization and high expression model mRNA-GFP molecule tissues...
Nucleic acid-based influenza vaccines are a promising platform that have recently and rapidly developed. We previously demonstrated the immunogenicity of DNA encoding artificial immunogens AgH1, AgH3, AgM2, which contained conserved fragments hemagglutinin stem two subtypes A—H1N1 H3N2—and protein M2. Thus, aim this study was to design characterize modified mRNA obtained using above plasmid as template. To select most protocol for creating highly immunogenic vaccines, we performed...
When developing drugs against SARS-CoV-2, it is important to consider the characteristics of patients with different co-morbidities. People infected HIV-1 are a particularly vulnerable group, as they may be at higher risk than general population contracting COVID-19 clinical complications. For such patients, broad spectrum antiviral activity paramount importance. Glycyrrhizinic acid (Glyc) and its derivatives promising biologically active compounds for development broad-spectrum agents. In...
The development of a safe and effective vaccine against avian influenza A virus (AIV) H5N8 is relevant due to the widespread distribution this in bird population existing potential risk human infection, which can lead significant public health concerns. Here, we developed an experimental pVAX-H5 DNA encoding modified trimer AIV hemagglutinin. Immunization BALB/c mice with using jet injection elicited high titer antibody response (the average ELISA was 1 × 105), generated level neutralizing...
In this work, we evaluated the antiviral activity of Arbidol (Umifenovir) against SARS-CoV-2 using a pseudoviral system with glycoprotein S virus on its surface. order to search for binding sites protein virus, described alternative in RBD and ACE-2-RBD complex. As result our molecular dynamics simulations combined docking data, note following fact: wherever molecules bind, interaction latter affects structural flexibility protein. This may both change shape domain-enzyme interface simply...
The construction of artificial proteins using conservative B-cell and T-cell epitopes is believed to be a promising approach for vaccine design against diverse viral infections. This article describes the development an HIV-1 immunogen polyepitope strategy. We developed recombinant protein, referred as nTBI, that contains recognized by broadly neutralizing antibodies (bNAbs) combined with Th-epitopes. modified version previously designed TBI (T- containing Immunogen), carrying four T- five...
The analysis of a pol gene fragment encoding protease and part reverse transcriptase was carried out for 55 sera collected in 2016 2018 from HIV-1-infected patients diagnosed 2014-2018 living the south Western Siberia, Russia: Altai Territory (n = 11), Republic 15), Kemerovo region 18), Novosibirsk 11). CRF63_02A dominant genetic form (>70%) regions, with subsubtype A6 comprising <30% samples. In Republic, predominant (53%), 33% viruses belonging to CRF63_02A. Four CRF63_02A/A6 unique...
Viruses can infect all types of life forms, from animals and plants to microorganisms, including bacteria archaea. When studying samples containing viruses, one confronts an unavoidable question the quantitative determination viral particles in sample. One simplest efficient approaches preparation includes use electron microscopy; however, a high detection threshold is significant limitation this method (107 per ml). Usually, such sensitivity insufficient result error diagnosis. This study...
BACKGROUND: Pseudovirus technology is a versatile and valuable tool for both fundamental applied virological research. Pseudotyped viruses provide the same cell entry mechanism as SARS-CoV-2 are widely used to investigate virus mechanism, tropism, neutralization assays. AIM: The aim of work obtain pseudotyped evaluate their transducing activity. MATERIALS AND METHODS: Using genetic engineering methods, construct carrying glycoprotein S gene was obtained, well pLenti-Luc-GFP reporter plasmid...
Quick label-free virus screening and highly sensitive analytical tools/techniques are becoming extremely important in a pandemic. In this study, we developed biosensing device based on the silicon nanoribbon multichannel dielectrophoretic controlled sensors functionalized with SARS-CoV-2 spike antibodies for use as platform detection studding of properties viruses their protein components. Replicatively defective viral particles vesicular stomatitis HIV-1 were used carrier molecules to...
Introduction. Obtaining stabilized recombinant HIV-1 Env trimers that have a close to the native conformation is one of directions in field development vaccines against HIV-1. The aim study was obtain and characterize trimer SOSIP.664 based on circulating genetic variant form CRF63_02A6. Materials methods. Bioinformatics resources were used design gene designed synthesized cloned as part an integration plasmid vector. A stable producer obtained by transfection CHO-K1 cell line using...
Highly pathogenic avian influenza (HPAI) H5 clade 2.3.4.4b viruses are widespread in wild and domestic birds, causing severe economic damage to the global poultry industry. Moreover, of this can adapt mammals, posing a potential pandemic threat. With constant evolution change dominant strains 2.3.4.4b, it is important investigate cross-reactivity available developing vaccine preparations. In study, immuno-genicity previously developed DNA encoding modified hemagglutinin influ-enza...
This work provides the results of a comparative study two T-cell polyepitope immunogens AG1 and AG4 encoding cytotoxic T-helper epitopes from NS1, NS3, NS5 E proteins tick-borne encephalitis virus. The integration alanine spacers into construct designed with PolyCTLDesigner was shown to increase its immunogenicity
HIV infection still remains a major challenge for healthcare systems of the world. There are several aspects on counteracting HIV/AIDS epidemic. The f irst aspect covers preventive measures including educational campaigns and promotion healthy lifestyle, protected sex, pre-exposure prophylaxis vulnerable groups. second is timely testing use antiretroviral therapy when test results come back positive. third scientif ic research associated with discovering new pharmaceutical agents developing...
The human immunodeficiency virus (HIV-1) poses a serious risk to global public health. development of safe and effective vaccine could stop the HIV/AIDS pandemic. Much research focused on HIV-1 prevention through vaccination is aimed at developing immunogens immunization strategies induce formation antibodies with neutralizing activity against broad range isolates (bNAbs). objective this study was develop capable targeting an immune response MPER, one regions bNAb binding in Env. Two...