A5007419835- Glioma Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Brain Metastases and Treatment
- Cancer Research and Treatments
- Health Literacy and Information Accessibility
- Cancer-related Molecular Pathways
- Groundwater flow and contamination studies
- Social Media in Health Education
- Phosphorus and nutrient management
- Chronic Lymphocytic Leukemia Research
- Brain Tumor Detection and Classification
- Misinformation and Its Impacts
- Soil and Unsaturated Flow
- HIV-related health complications and treatments
- HIV Research and Treatment
- Computational Drug Discovery Methods
- Genetic Associations and Epidemiology
- Immunodeficiency and Autoimmune Disorders
- Bioinformatics and Genomic Networks
- Landfill Environmental Impact Studies
- Coagulation and Flocculation Studies
- Heavy metals in environment
- Melanoma and MAPK Pathways
- HIV/AIDS Research and Interventions
- Genetics, Bioinformatics, and Biomedical Research
Dana-Farber Cancer Institute
2024-2025
Harvard University
2024
Saint Joseph University
2020-2023
Rwanda Meteorological Service
2022
American University of Beirut
1997-2014
American University of Beirut New York Office
2000-2001
<p>Supplementary Figure 7. Cellular Neighborhood Analyses. Comparison of annotated cellular neighborhoods in pre- and post-treatment tumor samples via CODEX (color code as 4).</p>
<p>Supplementary Figure 3. Digital quantification from immunohistochemistry. immunohistochemistry for T cell subsets and marker analysis paired tumor samples five patients enrolled to cohort A.</p>
<p>Supplementary Figure 6. Cell-cell interaction analyses. comparison in pre- and post-treatment tumor samples using CODEX for patient #5. (A) All significant interacting cell types shown the entire analyzed region as annotated. (B) Example images of – interactions showing CD163+ macrophages with GZMB+ CD8 T cells, CD4 CD68+ cells. The plus sign indicates that were close proximity at a one-cell distance (20 pixels).</p>
<p>Supplementary Figure 2. Patient enrollment, treatment and disposition per cohort.</p>
<p>Supplementary Figure 4. CODEX analysis of tumor PD-L1 expression patterns. (A) was detected in NCAM-1+ cells surrounding by hypoxia region as CA9 staining. (B) also observed GFAP+ surrounded the vicinity apoptotic (cleaved caspase-3 detection).</p>
<div>AbstractPurpose:<p>Radiotherapy may enhance antitumor immune responses by several mechanisms, including induction of immunogenic cell death. We performed a phase 2 study pembrolizumab with re-irradiation in patients recurrent glioblastoma.</p>Patients and Methods:<p>Sixty glioblastoma received alone (cohort A, bevacizumab-naïve; <i>n</i> = 30) or bevacizumab continuation B, 30). Dual primary endpoints, the overall response rate survival (OS) at either...
<p>Supplementary Figure 1. CODEX panel and experimental procedure. used to identify tumor cell subpopulations, immune populations key cellular markers. (B) Experimental workflow for 5 pre- post-treatment FFPE samples. ROIs were selected multiplexed imaging downstream analysis. (C) U Map clusters show major populations. Immune showed similar clustering in post- treatment samples.</p>
<p>Supplementary Figure 5. Cell Density Analyses. Comparison of defined cell populations in pre- and post-treatment tumor specimens via CODEX. (A) Each indicated type is shown as a percentage total cells each post-tumor specimen for all five patients. (B) density T subpopulations preand patient. CD4 are different shades blue; CD8 red. was determined by count per microm2. increased substantially patient #5. (C) changes paired posttreatment samples. Patient #5 highlighted with blue...
Foundation models are reshaping computational pathology by enabling transfer learning, where pre-trained on vast datasets can be adapted for downstream diagnostic, prognostic, and therapeutic response tasks. Despite these advances, foundation still limited in their ability to encode the entire gigapixel whole-slide images without additional training often lack complementary multimodal data. Here, we introduce Threads, a slide-level model capable of generating universal representations any...
Inhibitors of murine double minute homolog 2 (MDM2) represent a promising therapeutic approach for the treatment TP53 wild-type glioblastomas (GBMs), reactivating p53 signaling to induce cancer cell death. We conducted surgical window-of-opportunity trial (NCT03107780) MDM2 inhibitor navtemadlin (KRT-232) in 21 patients with recurrent GBM determine achievable drug concentrations within tumor tissues and biological mechanisms response resistance. Participants received at 120 mg ( n = 10) or...
The effect of fast mixing on floc formation and pollutant removal, using magnesium hydroxide as a coagulant, was investigated through characterization relative strength size the formed flocs while operating at different speeds times dynamic optical monitoring apparatus, photometric dispersion analyzer (PDA2000). parameters included speed (80, 100, 120 rpm) time (20, 40, 60 s). Highly turbid kaolin clay suspensions (1213 ± 36 NTU) were alkalized sodium (NaOH) to pH values 10.51 0.02...
Diseases were initially thought to be the consequence of a single gene mutation. Advances in DNA sequencing tools and our understanding behavior have revealed that complex diseases, such as cancer, are product genes cooperating with each other their environment orchestrated communication networks. Seeing function individual is still used analyze shift using functionally interacting groups new unit study holds promise for demystifying cancer.The literature search focused on three types namely...
Aim: Chronic lymphocytic leukemia (CLL) is a highly heterogenous hemopathy. Genetic stratification of CLL patients has important prognostic and therapeutic values - mainly immunoglobulin heavy chain variable region gene (IGHV) mutational status the presence cytogenetic abnormalities. The genetics in Lebanon scarcely described literature. Patients & methods: In this work, we studied genetic biomarkers 312 Lebanese patients. Results: Prominent IGHV genes were IGHV4-34, IGHV1-69 IGHV3-30; #1 #5...
Solar extraction of common salt from seawater has been practiced for centuries in a rather crude manner because the process was not performed stages to obtain pure salt. The practice developing countries is carry through with evaporation stage dryness facilitate harvesting mix. If harvested prior deposition impurities during then resulting bittern would provide chemicals which could have many commercial applications particularly treatment industries. This paper describes an effective...
Abstract We investigated the effectiveness of navtemadlin (KRT-232) in treating recurrent glioblastoma. A surgical window-of-opportunity trial ( NCT03107780 ) was conducted on 21 patients to determine achievable drug concentrations within tumor tissue and examine mechanisms response resistance. Both 120 mg 240 daily dosing achieved a pharmacodynamic impact. Sequencing three tumors revealed an absence TP53 -inactivating mutations, indicating alternative In patient-derived GBM models, lower...
Abstract Alterations in Fibroblast growth factor receptor (FGFR)-family proteins frequently occur as oncogenes many cancers, including a subset of pediatric gliomas. Here, we performed genomic analysis 11,635 gliomas across ages and found that 4.5% all harbor FGFR alterations structural variants (SV) single nucleotide (SNV), with an incidence almost 10% family members are differentially enriched by age, tumor grade, histological subtype, FGFR1-alterations associated glioneuronal histologies...