3,3',5,5'-Tetramethylbenzidine (TMB) remains one of the most widely utilized chromogenic substrates for horseradish peroxidase (HRP) in colorimetric immunoassays, including enzyme-linked immunosorbent assays (ELISA). Despite its introduction into ELISA workflows over four decades ago, limited research has been conducted to systematically optimize TMB substrate formulations. Recent advancements field have proposed innovative approaches enhance HRP catalysis, such as use deep eutectic solvents...

Lanthanide complexes with organic ligands exhibit unique photophysical properties, including long-lived emission lifetimes, large Stokes shifts, and sharp bands, making them highly attractive for bioanalytical biomedical applications. Incorporating these into nanoparticles can further enhance signal intensity assay sensitivity in both vitro vivo settings. Here, we report the first successful use of desolvation method to synthesize bovine serum albumin (BSA) loaded europium complexes. This...

Nanozymes, while promising alternatives to natural peroxidases in colorimetric assays, are often hindered by lower catalytic efficiencies. While various strategies exist enhance signal intensity nanozyme-based substrate optimization remains largely underexplored. The vast majority of studies rely on standard sodium acetate buffers or commercially-sourced substrates optimized for horseradish peroxidase, neglecting the unique properties different nanozymes. This work presents a systematic...

Nanozymes, while promising alternatives to natural peroxidases in colorimetric assays, are often hindered by lower catalytic efficiencies. Although numerous approaches have been developed improve signal intensity nanozyme-based optimization of the reaction medium which nanozyme interacts with substrate remains a significantly underexplored area. The vast majority studies rely on standard sodium acetate buffers or commercially sourced reagents optimized for horseradish peroxidase, neglecting...

Gelatin nanoparticles found numerous applications in drug delivery, bioimaging, immunotherapy, and vaccine development as well biotechnology food science. Synthesis of gelatin is usually made by a two-step desolvation method, which, despite providing stable homogeneous nanoparticles, has many limitations, namely complex procedure, low yields, poor reproducibility the first step. Herein, we present modified one-step which enables quick, simple, reproducible synthesis nanoparticles. Using...

1H-Pyrrole-2,3-diones, fused at [e]-side with a heterocycle, are suitable platforms for the synthesis of various angular polycyclic alkaloid-like spiroheterocycles. Recently discovered sulfur-containing [e]-fused 1H-pyrrole-2,3-diones (aroylpyrrolobenzothiazinetriones) tend to exhibit unusual reactivity. Based on these peculiar representatives 1H-pyrrole-2,3-diones, we have developed an approach unprecedented 6/5/5/5-tetracyclic spiroheterocyclic system...

The surface functionalization of magnetic nanoparticles improves their physicochemical properties and applicability in biomedicine. Natural polymers, including proteins, are prospective coatings capable increasing the stability, biocompatibility, transverse relaxivity (r2) nanoparticles. In this work, we functionalized nanoclusters carbon-coated iron with four proteins: bovine serum albumin, casein, gelatins A B, conducted a comprehensive comparative study essential to applications...

Multiple graphene-based therapeutics have recently been developed, however potential risks related to the interaction between nanomaterials and immune cells are still poorly understood. Therefore, studying impact of graphene oxide on various populations is importance. In this work, we aimed investigate effects PEGylated monocytes isolated from human peripheral blood. Graphene nanoparticles with lateral sizes 100-200 nm 1-5 μm were modified linear branched PEG (GO-PEG). Size, elemental...

Prussian blue nanozymes possessing peroxidase-like activity gather significant attention as alternatives to natural enzymes in therapy, biosensing, and environmental remediation. Recently, nanoparticles with enhanced catalytic prepared by reduction of FeCl3/K3[Fe(CN)6] mixture have been reported. These were denoted 'artificial peroxidase' nanozymes. Our study provides insights into the process their synthesis. We studied how size synthesis yield can be controlled via adjustment conditions....

One of the emerging trends in modern analytical and bioanalytical chemistry involves substitution enzyme labels (such as horseradish peroxidase) with nanozymes (nanoparticles possessing enzyme-like catalytic activity). Since enzymes typically operate through different mechanisms, it is expected that optimal reaction conditions will also differ. The optimization substrates for usually focuses on determining ideal pH temperature. However, some cases, even this step overlooked, commercial...

Prussian blue nanozymes exhibit peroxidase-like catalytic activity and are therefore considered a stable inexpensive alternative to natural peroxidases in the enzyme-linked immunosorbent assay (ELISA). In this work, we propose robust method of nanozyme functionalization, which relies on entrapment into albumin nanoparticles. The principle is addition ethanol solution that contains nanozymes. At high concentration solubility decreases, resulting formation nanoparticles loaded with...

Point-of-care tests play an important role in serological diagnostics of infectious diseases and post-vaccination immunity monitoring, including COVID-19. Currently, lateral flow dominate this area show good analytical performance. However, studies to improve the effectiveness such remain important. In comparison with tests, vertical immunoassays allow for a reduction assay duration influence hook effect. Additionally, use carbon black nanoparticles (CNPs) as color label can provide lower...

Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Twitter Facebook Reddit LinkedIn Tools Reprints and Permissions Cite Search Site Citation S. Uzhviyuk, M. Bochkova, V. Timganova, P. Khramtsov, K. Shardina, Vlasova, E. Saidakova, Zamorina; PEGylated graphene oxide monocyte metabolism. AIP Conf. Proc. 20 February 2024; 2924 (1): 050005. https://doi.org/10.1063/5.0182629 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley...

Background: LaNiO3 perovskite nanoparticles, especially nanospheres (LNNS), show great promise in biomedical assays due to their peroxidase-like catalytic properties. Methods for synthesizing nanoparticles of various shapes have been developed, with spherical and rod-shaped showing better stability. However, LNNS-based diagnostic reagents not tested nanozyme enzyme-linked immunosorbent assay (NLISA) or other assays, there is limited data on synthesis. To fill this gap, it necessary develop a...

Abstract Background Pregnancy-specific β1-glycoproteins are capable of regulating innate and adaptive immunity, exerting predominantly suppressive effects. In this regard, they interest in terms their pharmacological potential for the treatment autoimmune diseases post-transplant complications. The effect these proteins on main pro-inflammatory subpopulation T lymphocytes, IL-17-producing helper cells (Th17), has not been comprehensively studied. Therefore, effects native pregnancy-specific...

Point-of-care tests play an important role in serological diagnostics of infectious diseases and post-vaccination immunity monitoring, including COVID-19. Currently, lateral flow dominate this area show good analytical performance. However, studies to improve the effectiveness such remain important. In comparison with tests, vertical immunoassays allow for a reduction assay duration influence hook effect. Additionally, use carbon black nanoparticles (CNP) as color label can provide lower...

Pregnancy-specific ?-1-glycoproteins (PSGs) are capable of regulating innate and adaptive immunity. As fetal antigens circulate in the blood pregnant women, it is particular interest to reveal effects PSGs on differentiation memory T cells context maternal-fetal tolerance formation. We studied if, native PSG preparation affects helper T-cell proliferation, frequencies CD4+CD45R0+, CD4+CD45RA+, CD4+CD45RA+CD45R0+cells, naive CD45RA+CD45R0-CD62L+ (NAIVE), central CD45RA-CD45R0+CD62L+ (TCM),...