A5020021148- Mast cells and histamine
- Cytomegalovirus and herpesvirus research
- Asthma and respiratory diseases
- Viral-associated cancers and disorders
- Herpesvirus Infections and Treatments
- Food Allergy and Anaphylaxis Research
- Sphingolipid Metabolism and Signaling
- Urticaria and Related Conditions
- RNA Research and Splicing
- Inflammasome and immune disorders
- Allergic Rhinitis and Sensitization
- interferon and immune responses
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Genomics and Chromatin Dynamics
- Eosinophilic Disorders and Syndromes
- Circadian rhythm and melatonin
- Immune Cell Function and Interaction
- Platelet Disorders and Treatments
- T-cell and B-cell Immunology
- Lymphatic System and Diseases
- Plant Molecular Biology Research
- Parvovirus B19 Infection Studies
- DNA Repair Mechanisms
National Institutes of Health
2015-2024
National Institute of Allergy and Infectious Diseases
2015-2024
Medical College of Wisconsin
2012-2017
Versiti Blood Center of Wisconsin
2012
Sphingosine 1-phosphate (S1P) and S1P receptors (S1PRs) regulate migration of lymphocytes out thymus to blood lymph nodes (LNs) efferent lymph, whereas their role in other tissue sites is not known. Here, we investigated the question how these molecules leukocyte from tissues through afferent lymphatics draining LNs (dLNs). S1P, but chemokines, selectively enhanced human murine CD4 T cell across lymphatic endothelial cells (LECs). S1PR1 S1PR4, LEC S1PR2, were required for LECs into vessels...
Gammaherpesviruses, such as Epstein-Barr virus (EBV), are ubiquitous cancer-associated pathogens that interact with DNA damage response, a tumor suppressor network. Chronic gammaherpesvirus infection and pathogenesis in response-insufficient host poorly understood. Ataxia-telangiectasia (A-T) is associated insufficiency of ataxia-telangiectasia mutated (ATM), critical response kinase. A-T patients display pattern anti-EBV antibodies suggestive controlled EBV replication; however, parameters...
ABSTRACT Gammaherpesviruses are ubiquitous pathogens that associated with the development of B cell lymphomas. employ multiple mechanisms to transiently stimulate a broad, polyclonal germinal center reaction, an inherently mutagenic stage differentiation is thought be primary target malignant transformation in virus-driven lymphomagenesis. We found this gammaherpesvirus-driven expansion was exaggerated and lost its transient nature absence interferon-regulatory factor 1 (IRF-1),...
ABSTRACT DNA damage response (DDR) is a sophisticated cellular network that detects and repairs breaks. Viruses are known to activate the DDR usurp certain components facilitate replication. Intriguingly, viruses also inhibit several proteins, suggesting this has both proviral antiviral features, with nature of latter still poorly understood. In study we show irradiation primary murine macrophages was associated enhanced expression interferon (IFN)-stimulated genes (ISGs). ISG induction in...
Summary Viruses such as E pstein– B arr virus ( EBV ) have been linked to mechanisms that support autoantibody production in diseases systemic lupus erythematosus. However, the by which viruses contribute remain poorly defined. This stems part, from high level of seropositivity for (> 95%) and exquisite species specificity . In this study we overcame these problems using murine gammaherpesvirus 68 MHV 68), a genetically biologically related We first showed drives promoting loss ‐cell...
Mast cells are key players in the development of inflammatory allergic reactions. Cross-linking high-affinity receptor for IgE (FcεRI) on mast leads to generation and secretion sphingolipid mediator, sphingosine-1-phosphate (S1P) which is able, turn, transactivate its receptors cells. Previous reports have identified expression two five S1P cells, S1P1 S1P2, with functions FcεRI-mediated chemotaxis degranulation, respectively. Here, we show that cultured mouse also express abundant message...
Manipulation of host cellular pathways is a strategy employed by gammaherpesviruses, including mouse gammaherpesvirus 68 (MHV68), in order to negotiate chronic infection. Ataxia-telangiectasia mutated (ATM) plays unique yet incompletely understood role infection, as it has both proviral and antiviral effects. Chronic infection poorly controlled with global ATM insufficiency, whether the or human. In contrast, facilitates replication, reactivation, latency establishment several gammaherpesviruses
Neoplastic accumulation of mast cells in systemic mastocytosis (SM) associates with activating mutations the receptor tyrosine kinase KIT. Constitutive activation oncogenes has been linked to imbalances oxidant/antioxidant mechanisms other myeloproliferative disorders. However, impact KIT on redox status SM and potential therapeutic implications are not well understood. Here, we examined regulation reactive oxygen species (ROS) antioxidant protein DJ-1 (PARK-7), which increases cancer...
The successful development of germinal centers (GC) relies heavily on innate mechanisms to amplify the initial inflammatory cascade. In addition their role in antigen presentation, cells are essential for redirection circulating lymphocytes toward draining lymph node (dLN) maximize surveillance. Sphingosine-1-Phosphate (S1P) and its receptors (S1PR1-5) affect various aspects immunity; however, S1PR4 regulating an immune response is not well understood. Here we use a footpad model localized T...
Abstract Mast cells are key players in the development of inflammatory allergic reactions. Cross-linking high-affinity receptor for IgE (FcɛRI) on mast leads to generation and secretion sphingolipid mediator, sphingosine-1-phosphate (S1P) which can turn activate its G-protein coupled receptors cells. Previous reports have identified expression two five S1P cells, S1P1 S1P2, with functions IgE-receptor-mediated chemotaxis degranulation, respectively. Here, we show that cultured human mouse...