A5088378568- Mesoporous Materials and Catalysis
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Enzyme Catalysis and Immobilization
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Nanopore and Nanochannel Transport Studies
- Mass Spectrometry Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Head and Neck Cancer Studies
- Birth, Development, and Health
- Analytical Chemistry and Chromatography
- Metal-Organic Frameworks: Synthesis and Applications
- Electrolyte and hormonal disorders
- DNA Repair Mechanisms
- DNA and Biological Computing
- Diatoms and Algae Research
- Glycosylation and Glycoproteins Research
- Pregnancy and preeclampsia studies
- Ion-surface interactions and analysis
- Hemoglobin structure and function
- Multiple and Secondary Primary Cancers
- Effects of Radiation Exposure
- Molecular Junctions and Nanostructures
- Polyamine Metabolism and Applications
National Institute of Advanced Industrial Science and Technology
2014-2025
Information Technology Institute
2016
Sendai University
2014
Tokyo University of Science
2007
Toyohashi University of Technology
1998-2004
Okayama University
2003
Osaka Sangyo University
1997
Gunma Children's Medical Center
1989
Inductively coupled plasma mass spectrometry (ICP-MS) is important in the biological and biochemical fields as it can quantify trace elements. Confocal laser Raman micros-copy (CLRM), a powerful tool for compositional analysis of samples, or-ganic materials, inorganic be used to analyze samples aqueous solutions. Despite their analytical strength, quantitative evaluation proteins bound mesoporous silica (SiO2) microspheres, which are promising candidates drug delivery systems vaccine...
Inductively coupled plasma mass spectrometry (ICP-MS) is important in the biological and biochemical fields as it can quantify trace elements. Confocal laser Raman microscopy (CLRM), a powerful tool for compositional analysis of samples, organic materials, inorganic be used to analyze samples aqueous solutions. Despite their analytical strength, quantitative evaluation proteins bound mesoporous silica (SiO2) microspheres, which are promising candidates drug delivery systems vaccine carriers,...
A fluorescence microscopy technique has been developed to visualize the behavior of individual DNA and protein molecules. Real-time direct observation a single molecule can be used investigate dynamics DNA–protein interactions, such as digestion reaction by λ exonuclease. In conventional methods it is impossible analyze an exonuclease on because they only observe average number Observation molecule, other hand, reveal processivity binding rate molecule. To evaluate exonuclease, stained with...
Single-particle inductively coupled plasma mass spectrometry (spICP-MS) has been used for particle size measurement of diverse types individual nanoparticles and micrometer-sized carbon-based particles, such as microplastics. However, its applicability to the non-carbon-based particles silica (SiO2) is unclear. In this study, spICP-MS non-porous/mesoporous SiO2 micro-spheres with a nominal diameter 5.0 µm or smaller was investigated. Particle sizes these microspheres were measured using both...
Message in a bottle: Alkaline hydrothermal treatment of lysozyme–silica hybrid particles leads to cagelike hollow aluminosilicates (CHAs) with vermiculate through-holes the shell. Biomacromolecules can be transported inside CHA through these holes and encapsulated within confined inner space by crosslinking ship-in-bottle manner (see picture). Detailed facts importance specialist readers are published as ”Supporting Information”. Such documents peer-reviewed, but not copy-edited or typeset....
A highly precise and sensitive technology that enables DNA amplification/detection from minimal amounts of nucleic acid is expected to find applicability in genetic testing involving small samples. The use a free enzyme conventional amplification techniques, such as the polymerase chain reaction (PCR), frequently causes side reactions (i.e., nonspecific amplification) when ≤103 substrate molecules are present, thereby preventing selective target DNA. To address this issue, we have developed...
Direct enzyme immobilization by encapsulation in the pores of mesoporous silica particles enhances protein thermal and chemical stability. In this study, we investigated effect pore size on thermostability catalytic activity Escherichia coli glutaminase YbaS encapsulated under high temperature conditions two SBA-type silicas: SBA5.4 SBA10.6 with diameters 5.4 10.6 nm, respectively. The changes conformation were assessed using PSA, a benzophenoxazine-based fluorescent dye that is sensitive to...
Based on real-time observation and micromanipulation, analytical methods for single DNA molecules have been under development some time. Precise manipulation, however, is still difficult because are too small conventional techniques. We developed a chemical reaction system that uses water droplets in oil as containers of materials. The can be manipulated by optical force. manipulation the permits fusion two selected droplets. This process corresponds to mixing different samples. designate...
A flow-type microreactor containing glutaminase-mesoporous silica composites with 10.6 nm pore diameter (TMPS10.6) was developed for the continuous synthesis of theanine, a unique amino acid. High enzymatic activity exhibited by local control reaction temperature.
A flow-type microreactor containing composites of the enzyme (glutaminase) and mesoporous silica microspheres with a 23.6 nm pore diameter (SBA23.6) exhibited precise, efficient, continuous synthesis theanine.
A new organic-inorganic hybrid zeotype compound with amphiphilic one-dimensional nanopore and aluminosilicate composition was developed. The framework structure is composed of double layers 12-ring nanopores; a hydrophilic layer pillared by Q(2) silicon atom species lipophilic phenylene groups are alternately stacked, nanopores perpendicularly penetrate the layers. topology looks similar to that an AFI-type zeolite but possesses quasi-multidimensional pore consisting channel intersecting...
The assembly and proper alignment of two heterofluorescent proteins (sGFP DsRed) in the mesoporous channels ethanol-treated FSM6.2 (a folded-sheet material with a pore diameter 6.2 nm) was confirmed using fluorescence resonance energy transfer (FRET) technique. sGFP-DsRed-FSM6.2 conjugate showed large decrease emission donor (sGFP) fluorescence, indicating that functions as an system through combination heteroproteins, due to successful encapsulation sGFP-DsRed pairs mesopores. Fluorescence...
In the present study, we examined reversible thermal deformation of membrane protein light-harvesting complex LH2 adsorbed on mesoporous silica (MPS) supports. The from Thermochromatium tepidum cells was conjugated to MPS supports with a series pore diameter (2.4 10.6 nm), and absorption spectra resulting LH2/MPS conjugates were observed over temperature range 273 – 313 K in order examine structure support. experimental results confirmed that slight ellipsoidal induced by adsorption On other...