A5016182563- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Epigenetics and DNA Methylation
- Microfluidic and Capillary Electrophoresis Applications
- Artificial Intelligence in Healthcare and Education
- Plant Genetic and Mutation Studies
- Bacteriophages and microbial interactions
- DNA and Biological Computing
- Nanopore and Nanochannel Transport Studies
- Radiomics and Machine Learning in Medical Imaging
- Neurogenesis and neuroplasticity mechanisms
- Carcinogens and Genotoxicity Assessment
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Force Microscopy Techniques and Applications
- Autopsy Techniques and Outcomes
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Advanced Biosensing Techniques and Applications
- Renal and Vascular Pathologies
- nanoparticles nucleation surface interactions
St. Marianna University School of Medicine
2023-2024
Scientific Electronic Library Online
2022
Sorbonne Université
2021
Genomic Vision (France)
2007-2016
Institut Curie
2010
Inserm
2010
ParisTech
2010
Centre National de la Recherche Scientifique
2010
Institut Pasteur
2007
Toyohashi University of Technology
2001-2006
Ataxia telangiectasia-mutated (ATM) and ataxia telangiectasia Rad3-related (ATR) kinases are two key regulators of DNA-damage responses (DDR) that mainly activated in response to DNA double-strand breaks single-stranded damages, respectively. Seckel syndrome, a rare genetic disorder characterized by microcephaly markedly reduced body size, has been associated with defective ATR-dependent damage signaling. However, the only human ATR defect reported so far is hypomorphic splicing mutation...
Neurogenesis in the developing human cerebral cortex occurs at a particularly slow rate owing part to cortical neural progenitors preserving their progenitor state for relatively long time, while generating neurons. How this balance between and neurogenic is regulated, whether it contributes species-specific brain temporal patterning, poorly understood. Here, we show that characteristic potential of cells (NPCs) remain as they generate neurons prolonged amount time requires amyloid precursor...
The multistage process of cancer formation is driven by the progressive acquisition somatic mutations. Replication stress creates genomic instability in mammals. Using a well-defined multistep leukemia model Spi-1/PU.1 overexpression mouse and Spi-1/PU.1-overexpressing human leukemic cells, we investigated relationship between DNA replication progression. Here, using molecular combing flow cytometry methods, show that Spi-1 increases speed acting specifically on elongation rather than...
The synthesis of rRNA is a key determinant normal and malignant cell growth subject to epigenetic regulation. Yet, the epigenomic features rDNA arrays clustered in nucleolar organizer regions are largely unknown. We set out explore for first time how DNA methylation distributed on individual arrays.Here we combined immunofluorescence detection modifications with fluorescence hybridization single fibers, metaphase immuno-FISH methylation-sensitive restriction enzyme digestions followed by...
Progress in sample preparation for scRNA-seq is reported based on RevGel-seq, a reversible-hydrogel technology optimized samples of fresh cells. Complexes one cell paired with barcoded bead are stabilized by chemical linker and dispersed hydrogel the liquid state. Upon gelation ice complexes immobilized physically separated without requiring nanowells or droplets. Cell lysis triggered detergent diffusion, RNA molecules captured adjacent beads further processing reverse transcription cDNA...
ABSTRACT Background Large language models (LLMs) pretrained on vast amounts of data have significantly influenced recent advances in artificial intelligence. While GPT-4 has demonstrated high performance general medical examinations, its specialised areas such as nephrology is unclear. This study aimed to compare ChatGPT and Bard their potential clinical applications nephrology. Methods Ninety-nine questions from the Self-Assessment Questions for Nephrology Board Renewal 2018 2022 were...
A microvortex is generated around an infrared laser focus where intense ac electric field applied. The authors used this optoelectrostatic for stretching individual long DNAs. When λ-or T4-phage DNA molecules were introduced into the microvortex, they stretched focus. In addition, especially longer T4 molecules, it was possible to keep in form more than 30s. Using method, length of can be measured without fixing a substrate. This method applied about 10μm.
Abstract We demonstrate an effective method for DNA immobilization on a hydrophobic glass surface. The new immobilizing technique is extremely simple compared with conventional techniques that require heterobifunctional crosslinking reagent between and substrate surface are both modified chemically. In the first process, coverslip was treated dichlorodimethylsilane resulting in γ molecules were ligated 3′-terminus disulfide-modified 14 mer oligonucleotides at one cohesive end. After...
Abstract This report shows a new DNA stretching method using migration of an ice-water interface. molecules were stretched accompanying the solid-liquid interface and immobilized in frozen area. simple needs no chemical modification to keep form. For full molecules, one terminus anchored on silanized substrate. The by freezing solution. observed after sublimation solution keeping its form surface which had attractive interaction with except for SH-modi- fied An infrared (IR) laser beam was...
In the current studies, we designed a new approach for sizing and isolating chromosomal DNA using coil-globule transition, which avoids fragmentation of giant due to mechanical stress. Although transition is reversible globular tolerant stress, cannot be manipulated by an electric field because loss its negative charges. our system, however, extracted from agarose gel in coiled state into solution PEG, induced cations generated at anode. This method achieves buffer exchange without stirring,...
Abstract The approximately 16 billion neurons of the human neocortex are derived from a relatively limited number developmental neural stem cells (NSCs). During embryogenesis, cortical NSCs initially generate at particularly slow rate while preserving their progenitor state for long time. How this balance between and neurogenic is regulated, whether it contributes to species-specific brain patterning, poorly understood. Here we show that characteristic potential remain in as they prolonged...
Labeling of DNA has been studied using base-specific Rhodium (II) acetate [Rh/sub 2/(O/sub 2/CCH/sub 3/)/sub 4/] (Rh1) compound. First, and Rh1 were mixed the binding reaction was checked by electrophoresis complex. The result showed that some molecules labeled Rh1. AFM observation also overlapped molecules. It suggests could be cross-linked on points.
An 81-year-old female with stage G3 chronic kidney disease and post left nephrectomy for upper tract urothelial carcinoma 20 years ago, was admitted to our hospital heart failure. She acutely developed right thigh pain, contrast-enhanced computed tomography (CT) revealed an idiopathic iliopsoas hematoma which transcatheter arterial embolization (TAE) performed. Subsequently, her hypertension worsened despite multiple antihypertensive medications (Nifedipine, Carvedilol, Doxazosin,...
<div>Abstract<p>The multistage process of cancer formation is driven by the progressive acquisition somatic mutations. Replication stress creates genomic instability in mammals. Using a well-defined multistep leukemia model Spi-1/PU.1 overexpression mouse and Spi-1/PU.1–overexpressing human leukemic cells, we investigated relationship between DNA replication progression. Here, using molecular combing flow cytometry methods, show that Spi-1 increases speed acting specifically on...
Supplementary Figure 3 from Spi-1/PU.1 Oncogene Accelerates DNA Replication Fork Elongation and Promotes Genetic Instability in the Absence of Breakage
Supplementary Figure 4 from Spi-1/PU.1 Oncogene Accelerates DNA Replication Fork Elongation and Promotes Genetic Instability in the Absence of Breakage
Supplementary Figure 2 from Spi-1/PU.1 Oncogene Accelerates DNA Replication Fork Elongation and Promotes Genetic Instability in the Absence of Breakage