A5054549556- DNA Repair Mechanisms
- RNA Research and Splicing
- Nanoplatforms for cancer theranostics
- Genomics and Chromatin Dynamics
- Advanced Biosensing Techniques and Applications
- Cancer therapeutics and mechanisms
- PARP inhibition in cancer therapy
- Luminescence and Fluorescent Materials
- Supramolecular Self-Assembly in Materials
- DNA and Nucleic Acid Chemistry
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Advanced NMR Techniques and Applications
- Photoreceptor and optogenetics research
- GABA and Rice Research
- Supramolecular Chemistry and Complexes
- Alzheimer's disease research and treatments
- Synthesis and Reactivity of Heterocycles
- RNA and protein synthesis mechanisms
- Gene expression and cancer classification
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- Nanoparticle-Based Drug Delivery
- Advanced Nanomaterials in Catalysis
- RNA regulation and disease
Unité en Sciences Biologiques et Biotechnologies de Nantes
2015-2023
Nantes Université
2015-2023
Centre National de la Recherche Scientifique
2015-2023
Bioénergétique et Ingénierie des Protéines
2020-2021
Université Paris Cité
2012-2018
Inserm
2012-2018
Sorbonne Paris Cité
2012-2015
Délégation Paris 5
2012-2015
Dynamique du noyau
2012
Institut des Maladies Génétiques Imagine
2012
Abstract Pericentromeric heterochromatin (PCH) gives rise to highly dense chromatin sub-structures rich in the epigenetic mark corresponding trimethylated form of lysine 9 histone H3 (H3K9me3) and protein 1α (HP1α), which regulate genome expression stability. We demonstrate that Tau, a involved number neurodegenerative diseases including Alzheimer’s disease (AD), binds localizes within or next neuronal PCH primary cultures from wild-type mice. Concomitantly, we show clustered distribution...
The capacity of endogenous Tau to bind DNA has been recently identified in neurons under physiological or oxidative stress conditions. Characterization the protein domains involved Tau-DNA complex formation is an essential first step clarifying contribution interactions neurological biological processes. To identify amino acid residues interaction with oligonucleotides, we have characterized a using nuclear magnetic resonance spectroscopy. Interaction AT-rich GC-rich 22 bp oligonucleotide...
Ataxia telangiectasia-mutated (ATM) and ataxia telangiectasia Rad3-related (ATR) kinases are two key regulators of DNA-damage responses (DDR) that mainly activated in response to DNA double-strand breaks single-stranded damages, respectively. Seckel syndrome, a rare genetic disorder characterized by microcephaly markedly reduced body size, has been associated with defective ATR-dependent damage signaling. However, the only human ATR defect reported so far is hypomorphic splicing mutation...
Tauopathies such as Alzheimer's Disease (AD) are neurodegenerative disorders for which there is presently no cure. They named after the abnormal oligomerization/aggregation of neuronal microtubule-associated Tau protein. Besides its role a protein, DNA-binding capacity and nuclear localization protein has been described in neurons. While questioning potential Tau-DNA binding development tauopathies, we have carried out large-scale analysis interaction with genome under physiological heat...
Strongly solvatochromic fluorophores are devised, containing alkyl chains and enable to self‐assemble as very bright fluorescent organic nanoparticles (FONs) in water ( Φ f = 0.28). The impart each fluorophore with strongly hydrophobic surroundings, causing distinct emission colors between FONs where the associated, their disassembled state. Such color change is harnessed assess long‐term fate of both cancer cells monocytes/macrophages. Disintegration orange‐emitting by monocytes/macrophages...
The expression and activity of DNA-dependent protein kinase (DNA-PK) is related to DNA repair status in the response cells exogenous endogenous factors. Recent studies indicate that Epidermal Growth Factor Receptor (EGFR) involved modulating DNA-PK. It has been shown a compound 4-nitro-7-[(1-oxidopyridin-2-yl)sulfanyl]-2,1,3-benzoxadiazole (NSC), bearing nitro-benzoxadiazole (NBD) scaffold, enhances tyrosine phosphorylation EGFR triggers downstream signaling pathways. Here, we studied...
EGF-bioconjugated fluorescent organic nanoparticles serve as bright labels to diagnose EGFR-overexpressing breast cancer cells.
Nuclear architecture as well gene nuclear positioning can modulate expression. In this work, we have analyzed the position of interferon-β (IFN-β) locus, responsible for establishment innate antiviral response, with respect to pericentromeric heterochromatin (PCH) in correlation virus-induced IFN-β Experiments were carried out two different cell types either non-infected (NI) or during time course three viral infections. NI cells, showed a monoallelic promoter association PCH that strongly...
RAD51 is the central protein in DNA repair by homologous recombination (HR), involved several steps of this process. It shown that overexpression correlated with increased survival cancer cells to treatments. For past decade, overexpression-mediated resistance has justified development targeted inhibitors. One first molecules described inhibit was 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid (DIDS) molecule. This small molecule effective inhibiting different functions RAD51, however...
Abstract Therapeutic efficacy against cancer is often based on a variety of DNA lesions, including double-strand breaks (DSBs) which are repaired by homologous recombination and non-homologous end joining (NHEJ) pathways. In the past decade, functions repair proteins have been described as potential mechanism resistance in tumor cells. Therefore, become targets to improve anticancer therapy. Given central role DNA-PKcs NHEJ, therapeutic targeting frequently strategy prevent treatment-induced...
Fluorescent organic nanoparticles (FONs), made of self-assembled solvatochromic fluorophores, represent a novel class bright imaging agents. On page 2727, E. Ishow and co-workers show how in cellulo studies evidence dramatic dissembling FONs by macrophages compared to cancer cells. Macrophages disintegrate internalized yellow-emitting expulse the individual components excreting vesicles. Strong green fluorescence arises from fluorophores dissolved rich lipid content vesicles (Cover design:...
ABSTRACT To maintain its genome integrity, the cell uses complementary and orchestrated processes, among which is DNA Damage Response. Pre mRNA maturation an essential step of damage response that provides adapted proteome in order to face genotoxic stress. We describe here a new phosphorylation RAD51 recombinase, on Ser97 residue. This Aurora A mediated modulates vitro activity evaluated by D-loop polymerization assays. Using recombinant proteins, we show RNA binding protein affinity ....